Synthesis · 3D Co-doped Ni-based conductive MOFs modified electrochemical sensor for highly sensitive detection of L-tryptophan

5 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Main

Route 1: Drop Cast

3 · 2.3.2

Metal precursorsCo-Ni-MOFs-1% powder

SolventsAnhydrous ethanol

Additives0.5% chitosan binder; 0.05 um alumina slurry used for electrode polishing

Atmosphereambient drying, not otherwise specified

Substrate orientationglassy carbon electrode surface

Work-up2 mg Co-Ni-MOFs-1% dispersed in 1 mL ethanol by ultrasonication for 30 min; polish GCE; wash in ethanol and water for 1 min each; dry naturally; pipette 5 uL suspension; dry at room temperature; cover with 2 uL 0.5% chitosan; store at 4 C.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Co-Ni-MOFs-1% material	2 mg · 2 mg mL^-1 suspension	3 · 2.3.2
Solvent	anhydrous ethanol	1 mL	3 · 2.3.2
Additive	chitosan	2 uL · 0.5%	3 · 2.3.2
Other	alumina slurry	0.05 um	3 · 2.3.2

Complete recipeSource: Main

Route 2: Solvothermal

3 · 2.3.1

Metal precursorsNi(NO3)2.6H2O, 2.3263 g; Co(NO3)2.6H2O added at Co/Ni molar ratios 0.1%, 1%, 2% and 5%

Linker precursorsTerephthalic acid (H2BDC), 0.6645 g

SolventsDMF, 30 mL for mixed metal solution plus 30 mL for linker solution

Additives500 uL hydrochloric acid

Atmospherenot specified

Temperature180

Time24

Work-upCool to room temperature; transfer to beaker; centrifuge at 8000 r min^-1; wash with DMF and anhydrous ethanol 5 times.

ActivationDried in vacuum drying oven for 12 h at 60 C; separate BET activation under vacuum at 150 C for 4 h before sorption measurement.

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O solid	2.3263 g	3 · 2.3.1
Metal Source	Co(NO3)2.6H2O solid	different amount · Co/Ni molar ratio 0.1%, 1%, 2%, 5%	3 · 2.3.1
Linker	Terephthalic acid (H2BDC)	0.6645 g	3 · 2.3.1
Solvent	N,N-dimethylformamide (DMF)	30 mL + 30 mL	3 · 2.3.1
Acid	hydrochloric acid	500 uL	3 · 2.3.1

Partial recipeSource: Both

Route 3: Drop Cast

2 · Supplementary figure captions · Fig. S3

Metal precursorsCo-Ni-MOFs-0.1%, Co-Ni-MOFs-1%, Co-Ni-MOFs-2% and Co-Ni-MOFs-5% powders

SolventsAnhydrous ethanol, inferred same as Co-Ni-MOFs-1%/GCE route

Additives0.5% chitosan binder, inferred same as Co-Ni-MOFs-1%/GCE route

Atmosphereambient drying, not otherwise specified

Substrate orientationglassy carbon electrode surface

Work-upElectrode labels in Fig. S3 indicate each Co/Ni-ratio powder was prepared as a GCE-modified electrode; exact fabrication not repeated beyond the Co-Ni-MOFs-1%/GCE procedure.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Co-Ni-MOFs ratio-series material	0.1%, 1%, 2%, 5% Co/Ni	2 · Supplementary figure captions · Fig. S3
Solvent	anhydrous ethanol	inferred 2 mg mL^-1 suspension from 1% route	2 · Supplementary figure captions · Fig. S3
Additive	chitosan	0.5%, inferred from 1% route	2 · Supplementary figure captions · Fig. S3

Complete recipeSource: Main

Route 4: Drop Cast

3 · 2.3.2

Metal precursorsNi-MOFs powder

SolventsAnhydrous ethanol

Additives0.5% chitosan binder

Atmosphereambient drying, not otherwise specified

Substrate orientationglassy carbon electrode surface

Work-upFabricated in the same way as Co-Ni-MOFs-1%/GCE.

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Ni-MOFs material	2 mg mL^-1 inferred same-way suspension	3 · 2.3.2
Additive	chitosan	2 uL inferred same-way addition · 0.5%	3 · 2.3.2

Complete recipeSource: Main

Route 5: Solvothermal

3 · 2.3.1

Metal precursorsNi(NO3)2.6H2O, 2.3263 g

Linker precursorsTerephthalic acid (H2BDC), 0.6645 g

SolventsDMF, 30 mL for Ni solution plus 30 mL for linker solution

Additives500 uL hydrochloric acid

Atmospherenot specified

Temperature180

Time24

Work-upCool to room temperature; transfer to beaker; centrifuge at 8000 r min^-1; wash with DMF and anhydrous ethanol 5 times.

ActivationDried in vacuum drying oven for 12 h at 60 C; separate BET activation under vacuum at 150 C for 4 h before sorption measurement.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O solid	2.3263 g	3 · 2.3.1
Linker	Terephthalic acid (H2BDC)	0.6645 g	3 · 2.3.1
Solvent	N,N-dimethylformamide (DMF)	30 mL + 30 mL	3 · 2.3.1
Acid	hydrochloric acid	500 uL	3 · 2.3.1