Sensing Application — Self-supporting electrochemical sensors for monitoring of cell-released H2O2 based on metal nanoparticle/MOF nanozymes

4 measurement groups · 17 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

In-situ amperometric detection of H2O2 released from living cells

Ag/2D Zn-MOF/GCE · Electrode

AA stimulation at 400 uM in 10 mL PBS; applied potential -0.55 V; H9C2 and HeLa cells.

Temperature: about 300
Geometry: Ag/Zn-MOF modified GCE in cell culture dish / electrochemical cell
Context: Ag/2D Zn-MOF composite sensor
Measurement source: main pp.8-9 · 3.7. In-situ and real-time detection · Fig. 6E-F; Fig. S10

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
H9C2 cell H2O2 amperometric response	approximately 0.26 uA response from Fig. 6F	—	visual estimate from bar plot	Visual Estimate Approximate	main p.8 · 3.7. In-situ and real-time detection · Fig. 6F
HeLa cell H2O2 amperometric responseMarked as a best value within this paper	approximately 0.51 uA response from Fig. 6F	—	visual estimate from bar plot	Visual Estimate Approximate	main p.8 · 3.7. In-situ and real-time detection · Fig. 6F

Chronoamperometric H2O2 sensing

Chronoamperometry

Ag/2D Zn-MOF/GCE · Electrode

Successive H2O2 additions in continuously stirred 0.1 M PBS at -0.55 V.

Temperature: about 300
Geometry: three-electrode electrochemical cell; Ag/AgCl reference; Pt wire auxiliary
Context: Ag/2D Zn-MOF composite sensor
Measurement source: main pp.7-8 · 3.5. Amperometric measurements of H2O2 · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
H2O2 linear range high segmentMarked as a best value within this paper	5 mM to 70 mM	—	—	Text Range	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5D
H2O2 linear range low segment	5 uM to 5 mM	—	—	Text Range	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5C
H2O2 limit of detectionMarked as a best value within this paper	1.67 uM (S/N = 3)	—	S/N = 3	Text Exact Reported	main p.8 · 3.5. Amperometric measurements of H2O2 · Fig. 5; Table S1
Optimised operating potentialMarked as a best value within this paper	-0.55 V vs Ag/AgCl	—	—	Text Exact Reported	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. S6
Calibration R2 high rangeMarked as a best value within this paper	R2 = 0.998	—	—	Text Exact Reported	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5D
Calibration R2 low range	R2 = 0.997	—	—	Text Exact Reported	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5C
H2O2 sensitivity high segment	358.7 uA mM-1 cm-2	—	—	Text Exact Reported	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5D
H2O2 sensitivity low segmentMarked as a best value within this paper	385.3 uA mM-1 cm-2	—	—	Text Exact Reported	main p.7 · 3.5. Amperometric measurements of H2O2 · Fig. 5C
Table S1 H2O2 linear rangeMarked as a best value within this paper	5-5000, 5000-70,000 uM	—	—	SI Table Range	SI p.S13 · S3. Additional Figures and Tables · Table S1
Table S1 H2O2 LODMarked as a best value within this paper	1.67 uM	—	—	SI Table Exact Reported	SI p.S13 · S3. Additional Figures and Tables · Table S1
Table S1 working potentialMarked as a best value within this paper	-0.55 V (vs. Ag/AgCl)	—	—	SI Table Exact Reported	SI p.S13 · S3. Additional Figures and Tables · Table S1

Repeatability, reproducibility and storage stability tests

Ag/2D Zn-MOF/GCE · Electrode

10 mM H2O2 in 0.1 M PBS at -0.55 V; five repeated measurements/electrodes and 6-day storage.

Temperature: about 300
Atmosphere: room temperature storage
Geometry: three-electrode electrochemical cell
Context: Ag/2D Zn-MOF composite sensor
Measurement source: main p.8 · 3.6. Repeatability, reproducibility, and stability · Figs. S7-S9

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Single-electrode repeatability RSD	0.79% (n = 5)	—	n = 5	Text Exact Reported	main p.8 · 3.6. Repeatability, reproducibility, and stability · Fig. S7
Five-electrode reproducibility RSD	0.65%	—	five separately prepared electrodes	Text Exact Reported	main p.8 · 3.6. Repeatability, reproducibility, and stability · Fig. S8
Current retention after storage	96.69% after 6 days	—	6 days room temperature	Text Exact Reported	main p.8 · 3.6. Repeatability, reproducibility, and stability · Fig. S9

Amperometric anti-interference/selectivity test

Chronoamperometry

Ag/2D Zn-MOF/GCE · Electrode

0.1 mM H2O2 and 2 mM Glu, AA, CA, NaCl, KCl in stirred 0.1 M PBS at -0.55 V.

Temperature: about 300
Geometry: three-electrode electrochemical cell
Context: Ag/2D Zn-MOF composite sensor
Measurement source: main p.8 · 3.5. Amperometric measurements of H2O2 · Fig. 5E-F

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Interferent tolerance list	2 mM Glu, AA, CA, NaCl and KCl show no obvious current compared with 0.1 mM H2O2	—	—	Text Qualitative	main p.8 · 3.5. Amperometric measurements of H2O2 · Fig. 5E-F; Table S1