Chronoamperometry with ATCh injections.
GC/Cu3(THQ)2/AChE biosensor · Electrode
Successive injection of 0.1 mM ATCh on each modified layer at 0.3 V.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|
Sensing Application — Dense Conductive Metal-Organic Frameworks as Robust Electrocatalysts for Biosensing
Measurement evidence
Sensing Application
Dense Conductive Metal-Organic Frameworks as Robust Electrocatalysts for Biosensing · Niu K., Sun P., Chen J. et al. · Analytical Chemistry · 2022 · 17177-17185
7 measurement groups · 29 results
Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.
Cyclic voltammetry of ATCh oxidation at AChE biosensors.
GC/Cu3(THQ)2/AChE biosensor · Electrode
1 mM ATCh and PBS buffer at GC/AChE, GC/Ni3(HHTP)2/AChE, GC/Cu3(HHTP)2/AChE, and GC/Cu3(THQ)2/AChE in 50 mM PBS, pH 7.4.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|---|---|---|---|---|
| GC/AChE ATCh oxidation operating potential | 0.85 V | — | — | Text Rounded Reported | main p.5, article p.17181 · Fabrication of Enzymatic Biosensing System · Figure 4a |
| GC/Cu3(HHTP)2/AChE ATCh oxidation potentialMarked as a best value within this paper | about 0.39 V | — | — | Text Approximate | main p.5, article p.17181 · Fabrication of Enzymatic Biosensing System · Figure 4a |
| GC/Cu3(THQ)2/AChE ATCh oxidation potentialMarked as a best value within this paper | about 0.39 V | — | — | Text Approximate | main p.5, article p.17181 · Fabrication of Enzymatic Biosensing System · Figure 4a |
| GC/Cu3(THQ)2/AChE sensitivity improvement versus GC/AChEMarked as a best value within this paper | 5 times higher | — | — | Text Rounded Reported | main p.5, article p.17181 · Fabrication of Enzymatic Biosensing System · Figure 4a |
| GC/Ni3(HHTP)2/AChE ATCh oxidation potential | 0.80 V | — | — | Figure Axis Approximate | main p.5, article p.17181 · Fabrication of Enzymatic Biosensing System · Figure 4a |
Selectivity/interference amperometric test.
GC/Cu3(THQ)2/AChE biosensor · Electrode
Common electroactive interferents measured at 0.3 V for GC/Cu3(THQ)2/AChE; GC/AChE control at 0.75 V in SI.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|
Amperometric current-concentration response and Michaelis-Menten analysis.
GC/Cu3(THQ)2/AChE biosensor · Electrode
Successive additions of ATCh; maximum response near 1 mM ATCh.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|---|---|---|---|---|
| Effective working area reproducibility RSD | 2.9% | — | — | Text Exact Reported | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S5b |
| Effective working area of Cu3(THQ)2-based biosensor | 0.037 cm2 | — | — | Text Exact Reported | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S5a |
| CV curve stability at high scan rate | 400 mV s-1 | — | — | Text Exact Reported | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S7 |
| Apparent Michaelis-Menten constant for GC/Cu3(THQ)2/AChEMarked as a best value within this paper | Km = 0.262 mM | — | — | Text Exact Reported | main p.6, article p.17182 · Sensing Mechanism · Figure S11 |
| Apparent Michaelis-Menten constant for GC/AChE control | Km = 0.296 mM | — | — | Figure Axis Rounded Reported | SI p.13, S-13 · Supporting Information · Figure S12 |
Chronoamperometric repeatability, device-to-device reproducibility, and storage stability.
GC/Cu3(THQ)2/AChE biosensor · Electrode
0.1 mM ATCh at optimum potential 0.3 V; 15 consecutive measurements; seven biosensors by one person and nine by three persons; storage at 4 C for 2 weeks.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|---|---|---|---|---|
| Optimum potential used for stability measurements | 0.3 V | — | — | Text Exact Reported | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S8 |
| Repeatability RSD for 15 consecutive chronoamperometric measurements | 1.1% | — | — | Text Exact Reported | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S9 |
| Seven biosensors prepared by one person RSD | 3.1% | — | — | Figure Axis Rounded Reported | SI p.11, S-11 · Supporting Information · Figure S10a |
| Nine biosensors prepared by three persons RSD | 3.9% | — | — | Figure Axis Rounded Reported | SI p.11, S-11 · Supporting Information · Figure S10b |
| Current retention after storage | over 95% after 2 weeks at 4 C | — | > | Text Approximate | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System |
Amperometric response comparison of AChE biosensors modified by different electrocatalysts.
GC/Cu3(THQ)2/AChE biosensor · Electrode
ATCh (1 mM) responses for cobalt phthalocyanine, Prussian Blue, MoS2, and Cu3(THQ)2-modified AChE biosensors.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|---|---|---|---|---|
| Cobalt phthalocyanine-modified AChE biosensor ATCh current response | 2.9 uA | — | — | Figure Axis Rounded Reported | SI p.7, S-7 · Supporting Information · Figure S6 |
| Cu3(THQ)2-modified AChE biosensor ATCh current responseMarked as a best value within this paper | 35.1 uA | — | — | Figure Axis Rounded Reported | SI p.7, S-7 · Supporting Information · Figure S6 |
| MoS2-modified AChE biosensor ATCh current response | 5.9 uA | — | — | Figure Axis Rounded Reported | SI p.7, S-7 · Supporting Information · Figure S6 |
| Prussian Blue-modified AChE biosensor ATCh current response | 1.9 uA | — | — | Figure Axis Rounded Reported | SI p.7, S-7 · Supporting Information · Figure S6 |
| Sensitivity increase versus prepared typical electrocatalystsMarked as a best value within this paper | 6-18 times | — | range | Text Range | main p.6, article p.17182 · Fabrication of Enzymatic Biosensing System · Figure S6 |
AChE inhibition-based paraoxon detection by amperometry.
GC/Cu3(THQ)2/AChE biosensor · Electrode
Paraoxon concentrations from 1 to 1000 ng mL-1; inhibition measured before/after exposure; AChE concentration selected as 20 mU uL-1; significant inhibition within 15 min.
| Property | Reported value | Normalised value | Uncertainty | Origin and quality | Source |
|---|---|---|---|---|---|
| Applied AChE concentration selected for paraoxon assay | 20 mU uL-1 | — | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure S14 |
| LOD below acceptable limitMarked as a best value within this paper | 27 times below its acceptable limit | — | — | Text Rounded Reported | main p.7, article p.17183 · Conclusions |
| Paraoxon calibration intercept | 17.32% | — | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5d |
| Paraoxon calibration R2 | R2 = 0.9617 | — | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5d |
| Paraoxon calibration slope | y = 23.24 lg x + 17.32 | — | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5d |
| EU maximum residue limit comparison value | 10 ng mL-1 | — | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon |
| Paraoxon linear detection rangeMarked as a best value within this paper | 1-1000 ng mL-1 | — | range | Text Range | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5d; Table S1 |
| Paraoxon limit of detectionMarked as a best value within this paper | 0.37 ng mL-1 | 0.37 ppb | — | Text Exact Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5d; Table S1 |
| Paraoxon inhibition response time | within 15 min; remains constant after fifteen min | — | — | Text Rounded Reported | main p.7, article p.17183 · Analytical Response of Paraoxon · Figure 5c; Table S1 |