Sensing Application — Conductive NiCo bimetal-organic framework nanorods with conductivity-enhanced electrochemiluminescence for constructing biosensing platform

5 measurement groups · 32 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

ECL miRNA-141 calibration

NiCo-HHTP/PtNP/H1/MCH biosensor electrode · Electrode

Biosensor placed in 2 mL 0.1 M PBS pH 7.0 containing 26 mM TPrA; ECL detection from -1.4 to 1 V at 0.3 V/s after output S1 and H3-Fc incubation.

Geometry: Modified glassy carbon electrode
Context: Composite biosensor built from NiCo-HHTP
Measurement source: 5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
miRNA-141 linear response range lower boundMarked as a best value within this paper	1 fM	1e-15 M	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4
miRNA-141 linear response range upper boundMarked as a best value within this paper	10 nM	1e-8 M	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4
miRNA-141 detection limitMarked as a best value within this paper	0.69 fM	6.9e-16 M	—	Text Exact Reported	19 · S-2.16 Limit of detection (LOD) calculation
Calibration equation intercept	22365.58	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4B
Calibration R2Marked as a best value within this paper	R2 = 0.9994	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4B
Calibration RSD at 100 fM miRNA-141	3.32% (100 fM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 100 pM miRNA-141	1.60% (100 pM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 10 fM miRNA-141	1.80% (10 fM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 10 nM miRNA-141	1.22% (10 nM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 10 pM miRNA-141	2.83% (10 pM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 1 fM miRNA-141	3.35% (1 fM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 1 nM miRNA-141	1.23% (1 nM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration RSD at 1 pM miRNA-141	0.69% (1 pM)	—	—	Caption Exact Reported	5 · Figure caption · Fig. 4
Calibration equation slope	1251.58 per log concentration	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 4B
Blank-sample standard deviation for LOD	sB = 1132.49	—	—	Text Exact Reported	19 · S-2.16 Limit of detection (LOD) calculation
Smallest detectable signal for LOD	Delta IL = 3397.47	—	—	Calculated From Reported Exact Reported	19 · S-2.16 Limit of detection (LOD) calculation
Table S4 this-work detection limitMarked as a best value within this paper	0.69 fM	6.9e-16 M	—	SI Table Exact Reported	S18 · S-2.14 Comparison of different methods for miRNA-141 detection · Table S4

ECL selectivity test

NiCo-HHTP/PtNP/H1/MCH biosensor electrode · Electrode

Interferents miRNA-21, miRNA-155, miRNA-199a and TBA at 1 nM; target miRNA-141 at 10 pM.

Geometry: Modified glassy carbon electrode
Context: Composite biosensor specificity
Measurement source: 5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
miRNA-141 specificity	good specificity; interferents gave high ECL and target/mixed samples gave low ECL	—	—	Text Qualitative	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5A

Consecutive scan stability, storage stability and reproducibility tests

NiCo-HHTP/PtNP/H1/MCH biosensor electrode · Electrode

Stability at 100 fM and 1 nM miRNA-141 over 10 consecutive scans; 1 pM treated biosensor stored at 4 C for 7 days; reproducibility tested at 1 pM miRNA-141.

Geometry: Modified glassy carbon electrode
Context: Composite biosensor analytical stability
Measurement source: 5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5B-C; Fig. S16

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Inter-assay reproducibility RSDMarked as a best value within this paper	1.09%	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5C
Intra-assay reproducibility RSDMarked as a best value within this paper	1.61%	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5C
Consecutive scan stability RSD at 100 fM miRNA-141Marked as a best value within this paper	1.21%	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. 5B
Consecutive scan stability RSD at 1 nM miRNA-141Marked as a best value within this paper	1.19%	—	—	Text Exact Reported	5 · 3.5 ECL performance of the fabricated biosensor · Fig. S16A
Seven-day storage ECL signal retentionMarked as a best value within this paper	about 93.99% of the initial value	—	—	Text Approximate	5 · 3.5 ECL performance of the fabricated biosensor · Fig. S16B

ECL response to cancer-cell lysates

NiCo-HHTP/PtNP/H1/MCH biosensor electrode · Electrode

Human prostate carcinoma 22Rv1 and breast cancer MCF-7 cell lysates; cell numbers 10^1 to 10^5.

Geometry: Modified glassy carbon electrode
Context: Composite biosensor practical sample test
Measurement source: 5 · 3.6 Preliminary application in cancer cells for miRNA-141 determination · Fig. 5D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Cancer-cell lysate ECL trend	22Rv1 ECL decreased from 10^1 to 10^5 cells; MCF-7 retained relatively high ECL over 10^1 to 10^5 cells	—	—	Text Qualitative	5 · 3.6 Preliminary application in cancer cells for miRNA-141 determination · Fig. 5D

Optimisation of NiCo-HHTP concentration, TPrA concentration, pH, voltage and incubation times

NiCo-HHTP/PtNP/H1/MCH biosensor electrode · Electrode

Optimised NiCo-HHTP at 1 ug/mL, TPrA at 26 mM, PBS pH 7.0, scan potential -1.4 to 1 V, Exo III reaction time 1 h and output S1/H3-Fc incubation time 2 h.

Geometry: Modified glassy carbon electrode
Context: Composite biosensor operating conditions
Measurement source: 15-18 · S-2.13 Optimization of experimental conditions · Fig. S13-S15

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimal Exo III target-recycling reaction timeMarked as a best value within this paper	1 h	—	—	Text Exact Reported	17 · S-2.13 Optimization of experimental conditions · Fig. S15A
Optimal NiCo-HHTP concentration on GCEMarked as a best value within this paper	1 ug/mL	—	—	Text Exact Reported	15 · S-2.13 Optimization of experimental conditions · Fig. S13A
Optimal PBS pHMarked as a best value within this paper	pH 7.0	—	—	Text Exact Reported	15 · S-2.13 Optimization of experimental conditions · Fig. S13C
Optimal output S1 and H3-Fc incubation timeMarked as a best value within this paper	2 h	—	—	Text Exact Reported	17 · S-2.13 Optimization of experimental conditions · Fig. S15B
ECL emission under 0 to 1 V scan	668 nm	—	—	Figure Axis Rounded Reported	S17 · S-2.13 Optimization of experimental conditions · Fig. S14D
Optimised scan-potential upper limitMarked as a best value within this paper	1 V	—	—	Text Exact Reported	16 · S-2.13 Optimization of experimental conditions · Fig. S14B
Optimised scan-potential lower limitMarked as a best value within this paper	-1.4 V	—	—	Text Exact Reported	16 · S-2.13 Optimization of experimental conditions · Fig. S14B
Optimal TPrA concentrationMarked as a best value within this paper	26 mM	0.026 M	—	Text Exact Reported	15 · S-2.13 Optimization of experimental conditions · Fig. S13B