Synthesis · Conductive NiCo bimetal-organic framework nanorods with conductivity-enhanced electrochemiluminescence for constructing biosensing platform

4 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: SI

Route 1: Hydrothermal

5 · S-1.3 Synthesis of Ni-HHTP

Metal precursorsNi(OAc)2.4H2O (10 mg)

Linker precursorsHHTP (7 mg)

Solvents4 mL deionized water

Atmospherenot specified

Temperature85

Time24

Work-upCollected by centrifugation, washing and drying.

Activationnone reported

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(OAc)2.4H2O	10 mg	5 · S-1.3 Synthesis of Ni-HHTP
Linker	HHTP	7 mg	5 · S-1.3 Synthesis of Ni-HHTP
Solvent	deionized water	4 mL	5 · S-1.3 Synthesis of Ni-HHTP

Complete recipeSource: Both

Route 2: Drop Cast

3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B

Metal precursorsPtNPs from H2PtCl6.6H2O precursor route in SI; NiCo-HHTP from main route

Linker precursorsH1 hairpin DNA; H3-Fc during detection

SolventsDeionized water washes; PBS during detection

AdditivesMCH; TPrA during ECL detection

Temperatureroom temperature for MCH blocking; 37 C for output S1/H3-Fc incubation during detection

TimeH1 overnight; MCH 50 min; output S1/H3-Fc 2 h

Substrate orientationPolished glassy carbon electrode

Oxidant / reductantTPrA coreactant for ECL detection

Work-upWashed with deionized water after each fabrication step.

Show 7 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	NiCo-HHTP	10 uL · 1 ug/mL	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Other	PtNPs	10 uL	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Other	H1	10 uL · 1 uM	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Other	MCH	10 uL · 1 mM	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Other	H3-Fc	10 uL · 2 uM	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Electrolyte	PBS	2 mL · 0.1 M, pH 7.0	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B
Other	TPrA	26 mM	3 · 2.2 Fabrication of the ECL biosensor · Scheme 1B

Complete recipeSource: Main

Route 3: Solvothermal

2 · 2.1 Synthesis of NiCo-HHTP

Metal precursorsNi(OAc)2.4H2O (7.5 mg); Co(OAc)2.4H2O (2.5 mg)

Linker precursorsHHTP (7 mg)

Solvents1 mL DMF/water (V:V = 1:1)

Atmospherenot specified

Temperature85

Time24

Work-upCollected via centrifugation, washing and drying.

Activationnone reported

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(OAc)2.4H2O	7.5 mg	2 · 2.1 Synthesis of NiCo-HHTP
Metal Source	Co(OAc)2.4H2O	2.5 mg	2 · 2.1 Synthesis of NiCo-HHTP
Linker	HHTP	7 mg	2 · 2.1 Synthesis of NiCo-HHTP
Solvent	DMF/water	1 mL; V:V = 1:1	2 · 2.1 Synthesis of NiCo-HHTP

Complete recipeSource: SI

Route 4: Other

5 · S-1.4 Synthesis of platinum nanoparticles (Pt NPs)

Metal precursorsH2PtCl6.6H2O (5 mg)

Solvents20 mL deionized water; final dispersion in 20 mL deionized water

Additivestrisodium citrate dehydrate (10 mg)

Atmospherenot specified

Temperatureroom temperature implied during sonication

Time0.5 h sonication before NaBH4 addition; 0.5 h mild sonication after addition

Oxidant / reductantNaBH4 aqueous solution (10 mg) as reductant

Work-upDark brown precipitates collected by centrifugation, washing and dispersed in 20 mL deionized water.

Activationnone reported

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	H2PtCl6.6H2O	5 mg	5 · S-1.4 Synthesis of platinum nanoparticles (Pt NPs)
Additive	trisodium citrate dehydrate	10 mg	5 · S-1.4 Synthesis of platinum nanoparticles (Pt NPs)
Solvent	deionized water	20 mL	5 · S-1.4 Synthesis of platinum nanoparticles (Pt NPs)
Reductant	NaBH4 aqueous solution	10 mg	5 · S-1.4 Synthesis of platinum nanoparticles (Pt NPs)