Spectroscopy — Bulk protonic conductivity in a cephalopod structural protein

Measurement evidence

Spectroscopy

Bulk protonic conductivity in a cephalopod structural protein · Ordinario D.D., Phan L., Walkup IV W.G. et al. · Nature Chemistry · 2014 · 596-602

1 measurement group · 4 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

SDS-PAGE, reverse phase HPLC, in-gel tryptic digestion and mass spectrometry

Wild-type reflectin A1 thin film on glass with gold electrodes · Thin Film

Wild-type and mutant proteins assayed for purity and sequence identity.

Context: purified wild-type and mutant reflectin proteins before film fabrication
Measurement source: 3 · Characterization of Wild Type and Mutant Reflectin A1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DE->A mutant mass-spectrometry sequence coverage	94%	—	—	Text Exact Reported	3 · Characterization · Supplementary Figure 9
Purified wild-type reflectin yield	>800 mg pure protein per litre of cell culture	—	—	Text Approximate	597 · Results
Random mutant mass-spectrometry sequence coverage	34%	—	—	Text Exact Reported	3 · Characterization · Supplementary Figure 11
Wild-type reflectin mass-spectrometry sequence coverage	94%	—	—	Text Exact Reported	3 · Characterization · Supplementary Figure 3