Construction of a portable and sensitive electrochemical immunosensor for the rapid detection of erythromycin based on semiconductive bimetallic MOF

4materials

7samples

4synthesis routes

16measurements

79results

5claims and caveats

Evidence map

Open a family to keep every result attached to its sample, method and conditions.

Sensing Application6 measurements49 results →Electrochemistry Application4 measurements13 results →Spectroscopy2 measurements8 results →Diffraction Structure1 measurement3 results →Microscopy Morphology1 measurement3 results →Porosity1 measurement3 results →Other1 measurement0 results →

Author interpretations and caveats

Paraphrased for this database from the authors’ stated interpretations — never quoted verbatim — and kept separate from reported measurements.

Application RelevanceSupport assessment: Medium

The SPE immunosensor was practically applicable to spiked drinking-water, pork and chicken samples, with recoveries broadly near the added ERY concentrations and similar HPLC comparison results.

Caveat: Real-sample evidence is from spiked samples; value_numeric in the detailed SI rows records found concentration while recovery/RSD or HPLC comparator values are retained in value_raw/uncertainty_raw.

S12-S15 · S4. Real samples analysis · Tables S1-S4 · Linked to 7 structured results

Application RelevanceSupport assessment: High

The CuxFe3-x(HHTP)2-based portable SPE immunosensor detects ERY over 1.0 fg mL-1 to 1.0 ng mL-1 with an ultralow LOD of 0.69 fg mL-1.

Caveat: Application result is for ERY sensing rather than intrinsic transport; raw calibration data are not available.

p.5-p.6 · 3.4. Quantitative detection · Fig. 3a-c; Table 1 · Linked to 3 structured results

Structure Property LinkSupport assessment: High

The bimetallic CuxFe3-x(HHTP)2 electrode has enhanced electrochemical activity relative to monometallic Cu3(HHTP)2 and Fe3(HHTP)2 controls.

Caveat: Monometallic control Rct values are stated in the main text; preparation recipes for the monometallic controls are not reported.

p.4 · 3.2. Electrochemical measurements · Fig. S4a · Linked to 3 structured results

Structure Property LinkSupport assessment: Medium

High porosity and surface area of CuxFe3-x(HHTP)2 provide abundant active sites for antibody immobilisation, supporting sensitive ERY detection.

Caveat: Surface-area and sensing data support the association, but antibody loading amount is not directly reported in the main text.

p.4-p.6 · 3.1 and 3.4 · Fig. 1g-h; Fig. 3 · Linked to 4 structured results

Transport MechanismSupport assessment: Medium

Mixed Cu+/Cu2+ and Fe2+/Fe3+ redox states are proposed to promote electron transfer, improving electrochemical conductivity and biosensing performance.

Caveat: Mechanistic inference from XPS valence assignments and electrochemical response; no direct bulk electrical conductivity value is reported.

p.4 · 3.1. Basic characterizations · Fig. 1i-j · Linked to 5 structured results

Material identities

Names and aliases are kept exactly within the paper’s own identity model.

Material	Composition	Structure context	Source
Cu3(HHTP)2 monometallic MOF controlBrowse family: Cu₃(HHTP)₂ / Cu–HHTP	Cu3(HHTP)2Cu centres in an HHTP conductive MOF. · 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP).	2D · PristinePreviously reported Cu3(HHTP)2 pattern used as a structural/electrochemical comparator.	p.4 · Results and discussion - Basic characterizations · Fig. 1e; Fig. S4a
Bimetallic CuxFe3-x(HHTP)2 semiconductive MOF	CuxFe3-x(HHTP)2Cu2+/Fe2+ coordination centres; XPS shows mixed Cu+/Cu2+ and Fe2+/Fe3+ states. · 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP).	2D · PristineSemiconductive graphene-like bimetallic HHTP framework; PXRD peaks match reported Cu3(HHTP)2 and Fe3(HHTP)2 patterns and HR-TEM shows low crystallinity.	p.2 · Introduction · Scheme 1
Fe3(HHTP)2 monometallic MOF controlBrowse family: Fe–HHTP family	Fe3(HHTP)2Fe centres in an HHTP conductive MOF. · 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP).	2D · PristinePreviously reported Fe3(HHTP)2 pattern used as a structural/electrochemical comparator.	p.4 · Results and discussion - Electrochemical measurements · Fig. S4a
Graphene/MWCNT/Ag-AgCl screen-printed electrode substrate	Not specified	unknown · CompositeScreen-printed three-electrode substrate; non-MOF application substrate for CuxFe3-x(HHTP)2 modification.	S5 · S1.4 Preparation of the screen-printed electrode (SPE)

Sample register

Sample form, processing state and composition status define the context for measurements.

Show 7 sample records

Sample	Form and role	Processing and geometry	Source
Bare screen-printed electrode before MOF modificationresearch_0211__mat__mat_spe_graphene_mwcnt_agagcl	Electrode · Paper Level Unspecified · Composite	Graphene/MWCNT nanocomposite ink printed as working and counter electrodes; Ag/AgCl paste printed as reference electrode; annealed at 40 C for 1 h and stored under vacuum.Polyimide substrate	S5 · S1.4 Preparation of the screen-printed electrode (SPE)
BSA/Ab/CuxFe3-x(HHTP)2/SPE immunosensorresearch_0211__mat__mat_cuxfe3x_hhtp2	Electrode · Target Sample · Composite	CuxFe3-x(HHTP)2/SPE incubated with 100 ng mL-1 antibody for 40 min, blocked with 1.0 wt% BSA for 30 min, rinsed with PBS and stored at 4 C.Screen-printed electrode	p.3 · 2.2. Fabrication of the CuxFe3-x(HHTP)2-based immunosensor
Cu3(HHTP)2/AE monometallic MOF electrode controlresearch_0211__mat__mat_cu3_hhtp2	Electrode · Pristine Control · Composite	Preparation not reported; referenced through SI Fig. S4a as an electrochemical control.Bare gold electrode (AE)	p.4 · 3.2. Electrochemical measurements · Fig. S4a
CuxFe3-x(HHTP)2/AE modified gold electroderesearch_0211__mat__mat_cuxfe3x_hhtp2	Electrode · Target Sample · Composite	Constructed in the same way as the SPE immunosensor by replacing SPE with AE.Bare gold electrode (AE)	p.3 · 2.2. Fabrication of the CuxFe3-x(HHTP)2-based immunosensor
As-synthesised CuxFe3-x(HHTP)2 powderresearch_0211__mat__mat_cuxfe3x_hhtp2	Powder · Target Sample · Mixed Metal	Solvothermal product collected by centrifugation, washed with ethanol three times and vacuum dried at 60 C overnight.	p.3 · 2.1. Synthesis of CuxFe3-x(HHTP)2
CuxFe3-x(HHTP)2/SPE modified screen-printed electroderesearch_0211__mat__mat_cuxfe3x_hhtp2	Electrode · Composite Sample · Composite	5 uL of CuxFe3-x(HHTP)2 suspension drop-cast on SPE, washed with PBS and air dried.Screen-printed electrode with circular carbon working electrode, Ag/AgCl reference electrode and arc-shaped carbon counter electrode	p.3 · 2.2. Fabrication of the CuxFe3-x(HHTP)2-based immunosensor
Fe3(HHTP)2/AE monometallic MOF electrode controlresearch_0211__mat__mat_fe3_hhtp2	Electrode · Pristine Control · Composite	Preparation not reported; referenced through SI Fig. S4a as an electrochemical control.Bare gold electrode (AE)	p.4 · 3.2. Electrochemical measurements · Fig. S4a