Electrochemistry Application — Construction of a portable and sensitive electrochemical immunosensor for the rapid detection of erythromycin based on semiconductive bimetallic MOF

4 measurement groups · 13 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Cyclic voltammetry (CV)

Voltammetry

CuxFe3-x(HHTP)2/AE modified gold electrode · Electrode

CV curves of bare AE, CuxFe3-x(HHTP)2/AE, Ab/CuxFe3-x(HHTP)2/AE, BSA/Ab/CuxFe3-x(HHTP)2/AE and ERY/BSA/Ab/CuxFe3-x(HHTP)2/AE in PBS with 5 mM [Fe(CN)6]3-/4-.

Geometry: Gold electrode (AE) construction states.
Context: Composite MOF/electrode states compared with bare AE.
Measurement source: p.4-p.5 · 3.2. Electrochemical measurements · Fig. 2b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CV construction trend	Antibody adsorption weakens current density; BSA causes slight decrease; ERY recognition further declines peak current density.	—	—	Text Qualitative	p.4 · 3.2. Electrochemical measurements · Fig. 2b

Electrochemical impedance spectroscopy (EIS)

Impedance spectroscopy

CuxFe3-x(HHTP)2/AE modified gold electrode · Electrode

Nyquist plots in PBS (0.01 M, pH 7.4) containing 5 mM [Fe(CN)6]3-/4- for bare AE, MOF-modified AE and Ab/BSA/ERY construction states.

Geometry: Gold electrode (AE) modified with MOF and biomolecular layers.
Context: Composite MOF/electrode states compared with bare AE and monometallic MOF controls.
Measurement source: p.4 · 3.2. Electrochemical measurements · Fig. 2a; Fig. S4a

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Ab/CuxFe3-x(HHTP)2/AE Rct	1.04 kOhm	1040 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. 2a
Bare AE Rct	0.14 kOhm	140 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. 2a
BSA/Ab/CuxFe3-x(HHTP)2/AE Rct	1.08 kOhm	1080 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. 2a
Cu3(HHTP)2/AE Rct	0.64 kOhm	640 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. S4a
CuxFe3-x(HHTP)2/AE RctMarked as a best value within this paper	0.54 kOhm	540 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. 2a
ERY/BSA/Ab/CuxFe3-x(HHTP)2/AE Rct	1.39 kOhm	1390 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. 2a
Fe3(HHTP)2/AE Rct	0.8 kOhm	800 Ohm	—	Text Exact Reported	p.4 · 3.2. Electrochemical measurements · Fig. S4a

Chronoamperometry / instant current (I-t)

Chronoamperometry

BSA/Ab/CuxFe3-x(HHTP)2/SPE immunosensor · Electrode

Portable electronic device equipped with SPE electrode and smartphone; I-t at 0.6 V in PBS (0.01 M, pH 7.4), construction-state current over 60 s.

Geometry: SPE with carbon working/counter electrodes and Ag/AgCl reference electrode.
Context: Portable composite SPE immunosensor construction states.
Measurement source: p.3-p.5 · 2.3. Electrochemical measurements; 3.2. Electrochemical measurements · Fig. 2c; Fig. S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Ab/CuxFe3-x(HHTP)2/SPE current	0.89 uA	—	—	Text Exact Reported	p.5 · 3.2. Electrochemical measurements · Fig. 2c
Bare SPE current	0.11 uA	—	—	Text Exact Reported	p.5 · 3.2. Electrochemical measurements · Fig. 2c
BSA/Ab/CuxFe3-x(HHTP)2/SPE current	0.87 uA	—	—	Text Exact Reported	p.5 · 3.2. Electrochemical measurements · Fig. 2c
CuxFe3-x(HHTP)2/SPE currentMarked as a best value within this paper	1.03 uA within 60 s	—	—	Text Exact Reported	p.5 · 3.2. Electrochemical measurements · Fig. 2c
ERY detection current	0.62 uA	—	—	Text Exact Reported	p.5 · 3.2. Electrochemical measurements · Fig. 2c

CV and EIS electrochemical workstation conditions

Impedance spectroscopy Voltammetry

CuxFe3-x(HHTP)2/AE modified gold electrode · Electrode

CHI760E electrochemical workstation; conventional three-electrode system in PBS (0.01 M, pH 7.4) with 5.0 mM [Fe(CN)6]3-/4-; CV -0.2 to 0.8 V at 50 mV s-1; EIS 0.01 Hz to 100 kHz with 5 mV AC amplitude; tests repeated at least thrice.

Geometry: Modified AE working electrode, platinum slide counter electrode and Ag/AgCl (saturated KCl) reference electrode.
Context: Composite MOF/AE electrochemical tests.
Measurement source: S6 · S1.6 Electrochemical measurements

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source