Spectroscopy — Shielding against Unfolding by Embedding Enzymes in Metal-Organic Frameworks via a de Novo Approach

3 measurement groups · 5 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

CAT@ZIF-8 · Powder

CAT@ZIF-8 fluorescence after exposure to 6 M urea, 8 M urea, 0.1 M 3-AT, 80 deg C and 80% DMF.

Context: ZIF-8 enzyme-in-MOF composite used to avoid ZIF-90 fluorescence interference
Measurement source: 3 · Fluorescence spectroscopy · Figure 3b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
lambda_max, native CAT@ZIF-8	337 nm	—	—	Text Exact Reported	3 · Fluorescence spectroscopy · Figure 3
lambda_max shift under urea	significantly lower degree of shift than free CAT after 6 and 8 M urea	—	—	Text Qualitative	3 · Fluorescence spectroscopy · Figure 3b

Free CAT · Unknown

Free CAT fluorescence after exposure to 6 M urea, 8 M urea and 0.1 M 3-AT; lambda_max used as unfolding indicator.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
lambda_max after 6 M urea	343 nm	—	—	Text Exact Reported	3 · Fluorescence spectroscopy · Figure 3a
lambda_max after 8 M urea	348 nm	—	—	Text Exact Reported	3 · Fluorescence spectroscopy · Figure 3a
lambda_max, native free CAT	337 nm	—	—	Text Exact Reported	3 · Fluorescence spectroscopy · Figure 3

CAT@ZIF-90 · Powder

CAT@ZIF-90 dissolved in 0.2 M HCl, mixed with loading buffer, heated at 95 deg C for 5 min, run on 4% stacking/12% resolving gel at 20 mA.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source