Synthesis · Ultrasensitive levofloxacin electrochemical biosensor based on semiconducting covalent organic framework/poly-L-cysteine/triangular Ag nanoplates modified glassy carbon electrode

5 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Both

Route 1: Drop Cast

p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Metal precursorsTri-AgNP suspension

Linker precursorsTABQ-CHHO-COF/Poly-L-Cys/GCE

SolventsTri-AgNP suspension medium from aqueous synthesis

Temperatureroom temperature

Substrate orientationTABQ-CHHO-COF/Poly-L-Cys/GCE

Work-up4 uL Tri-AgNP suspension drop-cast, incubated, and dried at room temperature

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Tri-AgNP suspension	4 uL	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE
Other	TABQ-CHHO-COF/Poly-L-Cys/GCE	Not specified	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Complete recipeSource: Both

Route 2: Drop Cast

p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Linker precursorspre-synthesised TABQ-CHHO-COF

SolventsDMF dispersion; ethanol and double distilled water washes

Atmospherenitrogen gas flow for drying pretreated GCE

Time30 min sonication

Substrate orientation3 mm glassy carbon electrode

Work-upGCE polished with alumina powders, washed with ethanol and water, dried with nitrogen; 5 uL COF suspension drop-cast and dried under an infrared lamp

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	TABQ-CHHO-COF	2 mg · 2 mg/mL in DMF	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE
Solvent	DMF	1 mL	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE
Other	TABQ-CHHO-COF suspension	5 uL drop-cast	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Complete recipeSource: Both

Route 3: Electrochemical

p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Linker precursorspre-synthesised TABQ-CHHO-COF/GCE

Solventsaqueous cysteine solution

Additivescysteine (0.02 M, pH = 1.0)

Substrate orientationTABQ-CHHO-COF/GCE

Oxidant / reductantelectropolymerisation potential window -0.5 to 1.0 V

Work-upElectropolymerised at 100 mV/s for 25 cycles

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	cysteine	0.02 M, pH = 1.0	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE
Other	TABQ-CHHO-COF/GCE	Not specified	p004 · Fabrication of TABQ-CHHO-COF/Poly-L-Cys/Tri-AgNP/GCE

Complete recipeSource: Main

Route 4: Other

p003-p004 · Synthesis of TABQ-CHHO-COF

Linker precursorsCHHO (0.5 g, 1.6 mM); TABQ (0.4 g, 2.4 mM)

Solvents15 mL deoxygenated acetic acid/ethanol (v/v 1:1); workup with water, methanol, acetone, and methanol/water

Additivesacetic acid

Atmospherenitrogen atmosphere during charging; annealed under nitrogen

Temperatureroom temperature stirring, then 100 C, then 140 C, then 200 C anneal

Time0.5 h stirring, 48 h at 100 C, 6 h at 140 C, 24 h Soxhlet wash, 5 h anneal

Work-upRinsed with water, methanol, and acetone; Soxhlet washed with methanol/water for 24 h

ActivationAnnealed at 200 C for 5 h under nitrogen

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	cyclohexanehexone (CHHO)	0.5 g · 1.6 mM	p003-p004 · Synthesis of TABQ-CHHO-COF
Linker	tetraminophenone (TABQ)	0.4 g · 2.4 mM	p003-p004 · Synthesis of TABQ-CHHO-COF
Solvent	deoxygenated acetic acid/ethanol (v/v 1:1)	15 mL	p003-p004 · Synthesis of TABQ-CHHO-COF
Acid	acetic acid	in 15 mL acetic acid/ethanol mixture	p003-p004 · Synthesis of TABQ-CHHO-COF
Solvent	methanol and water	Soxhlet wash for 24 h	p003-p004 · Synthesis of TABQ-CHHO-COF

Complete recipeSource: Main

Route 5: Other

p004 · Synthesis of Tri-AgNP

Metal precursorsAgNO3 (100 uL, 0.05 M)

Solvents50 mL deionized water

AdditivesTSC (3 mL, 30 mM); PVP (3 mL, 0.7 mM)

Temperatureroom temperature

Time10 min before TSC addition; subsequent additions every 10 min; centrifugation 5 min repeated 6 times

Oxidant / reductantH2O2 (120 uL, 30 wt%); NaBH4 (300 uL, 0.1 M)

Work-upCentrifuged 5 min for 6 times at 10000 r/min to obtain 0.5 mL suspension

Show 6 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	AgNO3	100 uL · 0.05 M	p004 · Synthesis of Tri-AgNP
Solvent	deionized water	50 mL	p004 · Synthesis of Tri-AgNP
Additive	trisodium citrate (TSC)	3 mL · 30 mM	p004 · Synthesis of Tri-AgNP
Additive	PVP	3 mL · 0.7 mM	p004 · Synthesis of Tri-AgNP
Oxidant	H2O2	120 uL · 30 wt%	p004 · Synthesis of Tri-AgNP
Reductant	NaBH4	300 uL · 0.1 M	p004 · Synthesis of Tri-AgNP