Sensing Application — A multifunctional n-doped cu–mofs (N–cu–mof) nanomaterial-driven electrochemical aptasensor for sensitive detection of deoxynivalenol

6 measurement groups · 57 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

DPV calibration for DON

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

PBS 1 mmol L-1, pH 5.0; successive DON additions; DeltaI = Ip - Ip0.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor
Measurement source: p006 · 2.4 Analytical Performance · Figure 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Calibration equation intercept	DeltaI (uA) = 4.4996 + 2.2376 lgC_DON	—	—	Text Exact Reported	p006 · 2.4 Analytical Performance · Figure 4B
Calibration correlation coefficient R2	R2 = 0.9941	—	—	Text Exact Reported	p006 · 2.4 Analytical Performance · Figure 4B
Calibration equation slope	2.2376 uA per log10(ng mL-1)	—	—	Text Exact Reported	p006 · 2.4 Analytical Performance · Figure 4B
DON linear range upper boundMarked as a best value within this paper	0.02-20 ng mL-1	—	—	Text Range	p006 · 2.4 Analytical Performance · Figure 4B
DON linear range lower boundMarked as a best value within this paper	0.02-20 ng mL-1	—	—	Text Range	p006 · 2.4 Analytical Performance · Figure 4B
Limit of detection for DONMarked as a best value within this paper	0.008 ng mL-1 (S/N = 3)	—	—	Text Exact Reported	p006 · 2.4 Analytical Performance · Figure 4B
LOD signal-to-noise criterion	S/N = 3	—	—	Text Exact Reported	p006 · 2.4 Analytical Performance · Figure 4B

Differential pulse voltammetry

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

AP1/N-Cu-MOF/GCE in PBS containing 10 ng mL-1 DON; curve d in Figure 3.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor after DON binding
Measurement source: p005 · 2.2 Electrochemical Behaviors · Figure 3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DON concentration in Figure 3 test	10 ng mL-1 DON	—	—	Caption Exact Reported	p005 · Figure 3 caption · Figure 3
DON-restored peak current	approximately -10 uA from Figure 3 curve d	—	—	Figure Axis Approximate	p005 · 2.2 Electrochemical Behaviors · Figure 3
DON-restored oxidation peak potential	about 0.15 V	—	—	Text Approximate	p005 · 2.2 Electrochemical Behaviors · Figure 3

DPV optimisation

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

Optimised N-Cu-MOF amount, AP1 concentration, electrolyte pH, and incubation time in PBS containing 2 ng mL-1 DON.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor
Measurement source: p005 · 2.3 Optimization of Assay Conditions · Figure S6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimal AP1 concentration	10 umol L-1	—	—	Text Exact Reported	p005 · 2.3 Optimization · Figure S6B
Maximum DPV response at optimal AP1	approximately 4.8 uA	—	—	Figure Axis Approximate	p004 · Supporting Information · Figure S6B
Optimal incubation time	40 min	—	—	Text Exact Reported	p005 · 2.3 Optimization · Figure S6D
Optimal N-Cu-MOF dispersion concentration	4 mg mL-1	—	—	Text Exact Reported	p005 · 2.3 Optimization · Figure S6A
Maximum DPV response at optimal N-Cu-MOF	approximately 4.8 uA	—	—	Figure Axis Approximate	p004 · Supporting Information · Figure S6A
Optimal electrolyte pH	pH 5.0	—	—	Text Exact Reported	p005 · 2.3 Optimization · Figure S6C
Maximum DPV response at pH 5	approximately 4.8 uA	—	—	Figure Axis Approximate	p004 · Supporting Information · Figure S6C
Plateau DPV response after 40 min	approximately 4.75 uA	—	—	Figure Axis Approximate	p004 · Supporting Information · Figure S6D

DPV reproducibility

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

Five batches of sensors modified with N-Cu-MOF and five AP1/N-Cu-MOF-modified electrodes tested with 2 ng mL-1 DON.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor
Measurement source: p006 · 2.5 Selectivity and Reproducibility · Figure S7

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
RSD for five N-Cu-MOF sensor batches	2.1%	—	—	Text Exact Reported	p006 · 2.5 Selectivity and Reproducibility · Figure S7
DON concentration for electrode reproducibility	2 ng mL-1 DON	—	—	Text Exact Reported	p006 · 2.5 Selectivity and Reproducibility
RSD for five AP1/N-Cu-MOF-modified electrodes	6.43%	—	—	Text Exact Reported	p006 · 2.5 Selectivity and Reproducibility
Five-batch signal upper bound	15.56 uA-16.41 uA	—	—	Text Range	p006 · 2.5 Selectivity and Reproducibility · Figure S7
Five-batch signal lower bound	15.56 uA-16.41 uA	—	—	Text Range	p006 · 2.5 Selectivity and Reproducibility · Figure S7

DPV selectivity test

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

DON and co-occurring mycotoxins each at 2 ng mL-1; MIX contains AFB1, AFB2, FB1, FB2, OTA, ZEN, and DON.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor
Measurement source: p006-p007 · 2.5 Selectivity and Reproducibility · Figure 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
AFB1 selectivity response	approximately 0.03 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
AFB2 selectivity response	approximately 0.03 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
Concentration for each selectivity analyte	2 ng mL-1	—	—	Caption Exact Reported	p007 · Figure 5 caption · Figure 5
DON selectivity response	approximately 4.8 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
FB1 selectivity response	approximately 0.03 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
FB2 selectivity response	approximately 0.03 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
MIX selectivity response	approximately 4.45 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
OTA selectivity response	approximately 0.03 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5
ZEN selectivity response	approximately 0.25 uA from Figure 5	—	—	Figure Axis Approximate	p007 · 2.5 Selectivity and Reproducibility · Figure 5

Spiked wheat sample recovery by DPV aptasensor

Voltammetry

AP1-grafted N-Cu-MOF/GCE aptasensor · Electrode

DON-free wheat flour spiked at 0.05, 0.50, 2.50, and 5.00 ug kg-1 in triplicate; extracted with acetonitrile/water and diluted in PBS.

Geometry: AP1/N-Cu-MOF/GCE working electrode
Context: target sensor in real sample matrix
Measurement source: p007-p009 · 2.6 Applications to Real Samples; 3.5 Sample Preparation · Table 2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Wheat spike added DON 0.05	0.05 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat spike added DON 0.5	0.50 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat spike added DON 2.5	2.50 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat spike added DON 5	5.00 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Centrifugation speed	8000 r/min	—	—	Text Exact Reported	p009 · 3.5 Sample Preparation
Acetonitrile fraction in extraction solvent	84:16 v/v acetonitrile/water	—	—	Text Exact Reported	p009 · 3.5 Sample Preparation
Wheat flour sample mass	2.0 g	—	—	Text Exact Reported	p009 · 3.5 Sample Preparation
Extraction solvent volume	10 mL acetonitrile/water	—	—	Text Exact Reported	p009 · 3.5 Sample Preparation
Wheat found DON for 0.05 spike	0.051 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat found DON for 0.5 spike	0.530 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat found DON for 2.5 spike	2.389 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat found DON for 5 spike	4.990 ug kg-1	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat recovery for 0.05 spike	101.5%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat recovery for 0.5 spike	105.9%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat recovery for 2.5 spike	95.6%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat recovery for 5 spike	99.8%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Overall wheat recovery upper bound	95.6-105.9%	—	—	Text Range	p007 · 2.6 Applications to Real Samples · Table 2
Overall wheat recovery lower bound	95.6-105.9%	—	—	Text Range	p007 · 2.6 Applications to Real Samples · Table 2
Wheat RSD for 0.05 spike	5.59%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat RSD for 0.5 spike	3.54%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat RSD for 2.5 spike	4.7%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Wheat RSD for 5 spike	6.01%	—	—	Table Exact Reported	p007 · 2.6 Applications to Real Samples · Table 2
Overall wheat RSD upper bound	3.54-6.01%	—	—	Text Range	p007 · 2.6 Applications to Real Samples · Table 2
Overall wheat RSD lower bound	3.54-6.01%	—	—	Text Range	p007 · 2.6 Applications to Real Samples · Table 2
Ultrasonic extraction time	40 min	—	—	Text Exact Reported	p009 · 3.5 Sample Preparation