Synthesis · Glucose sensing performance of bimetallic MOFs CoFe-ZIF/CC for enzyme-free saliva sensor applications

6 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

Route 1: Other

2 · 2.2 Syntheses

Solventsethanol wash

AdditivesHCl soak

Atmospherenot reported

Temperatureroom temperature implied

Time2

Substrate orientationcarbon cloth support; orientation not reported

Work-upCleaned with ethanol several times.

Activationnone reported

Scalability contextNo carbon-cloth size or HCl concentration reported.

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Acid	hydrogen chloride (HCl, 99%)	soaked for 2 h · not reported	2 · 2.2 Syntheses
Solvent	ethanol	cleaned several times · not reported	2 · 2.2 Syntheses

Partial recipeSource: Main

Route 2: Other

2 · 2.4 Electrode preparation

Metal precursorspreformed Co0.95Fe0.05-ZIF/CC

AdditivesNafion solution, 5 uL

Atmosphereair implied

Temperatureroom temperature

Substrate orientation3 mm round Co0.95Fe0.05-ZIF/CC disk on centre of GCE

Work-upGCE polished with alumina powders, rinsed and dried.

Activationnone reported

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	round-like Co0.95Fe0.05-ZIF/CC	3 mm diameter disk	2 · 2.4 Electrode preparation
Additive	Nafion solution	5 uL	2 · 2.4 Electrode preparation

Partial recipeSource: Main

Route 3: Hydrothermal

2 · 2.2 Syntheses · Fig. 1; Fig. S1

Metal precursorsCo(NO3)2.6H2O, 7.26 mmol; Fe(NO3)3.9H2O, 0.40 mmol

Linker precursors2-methylimidazole (2-MIM), 15.71 mmol

Solventsmethanol, 60 mL

Additivesacid-treated carbon cloth substrate

Atmospherenot reported

Temperature120

Time4

Substrate orientationcarbon cloth added to Teflon reactor; orientation not reported

Work-upProduct cleaned by methanol three times.

ActivationDried at 60 deg C overnight.

Scalability contextYield reported as 1.34 mg cm-2; CC dimensions not reported in text.

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Co(NO3)2.6H2O	7.26 mmol	2 · 2.2 Syntheses · Fig. 1; Fig. S1
Metal Source	Fe(NO3)3.9H2O	0.40 mmol	2 · 2.2 Syntheses · Fig. 1; Fig. S1
Linker	2-methylimidazole (2-MIM)	15.71 mmol	2 · 2.2 Syntheses · Fig. 1; Fig. S1
Solvent	methanol	60 mL · 99.5%	2 · 2.2 Syntheses · Fig. 1; Fig. S1
Other	acid-treated carbon cloth	not reported	2 · 2.2 Syntheses · Fig. 1; Fig. S1

Partial recipeSource: Main

Route 4: Drop Cast

2 · 2.4 Electrode preparation

Metal precursorspreformed Co0.95Fe0.05-ZIF powder

Solventsdeionised water, 100 uL

AdditivesNafion, 900 uL

Atmosphereair implied

Temperatureroom temperature

Substrate orientationglassy carbon electrode centre

Work-upGCE polished with 1, 0.50 and 0.05 um alumina; rinsed with deionised water; dried at room temperature.

Activationnone reported

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Co0.95Fe0.05-ZIF powder	5 mg	2 · 2.4 Electrode preparation
Additive	Nafion	900 uL	2 · 2.4 Electrode preparation
Solvent	deionised water	100 uL	2 · 2.4 Electrode preparation

Partial recipeSource: Main

Route 5: Hydrothermal

2 · 2.2 Syntheses

Metal precursorsCo(NO3)2.6H2O, 7.26 mmol inferred from similar procedure; Fe(NO3)3.9H2O, 0.40 mmol inferred

Linker precursors2-methylimidazole (2-MIM), 15.71 mmol inferred

Solventsmethanol, 60 mL inferred

Additivesnone; carbon cloth omitted

Atmospherenot reported

Temperature120

Time4

Work-upCleaned by methanol three times inferred from similar procedure.

ActivationDried at 60 deg C overnight inferred from similar procedure.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Co(NO3)2.6H2O	7.26 mmol inferred	2 · 2.2 Syntheses
Metal Source	Fe(NO3)3.9H2O	0.40 mmol inferred	2 · 2.2 Syntheses
Linker	2-methylimidazole (2-MIM)	15.71 mmol inferred	2 · 2.2 Syntheses
Solvent	methanol	60 mL inferred · 99.5%	2 · 2.2 Syntheses

Partial recipeSource: Both

Route 6: Other

2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2

Metal precursorspreformed Co0.95Fe0.05-ZIF/CC disk

Solventsultrapure water, acetone and ethanol for PET cleaning; deionised water for KOH-PVA gel

Additivescarbon paste; silver/silver chloride paste; conducting resin; KOH; PVA

Atmosphereair implied

Temperature80 for printed PET drying; room temperature for gel preparation not otherwise reported

Substrate orientationCo0.95Fe0.05-ZIF/CC disk pasted on PET working electrode

Work-upPET cleaned three times with ultrapure water, acetone and ethanol; carbon paste printed and dried at 80 deg C; Ag/AgCl paste coated and dried; insulating film attached.

ActivationKOH-PVA gel coating; no further activation reported

Scalability contextPET electrode geometry shown schematically in SI Fig. S2 but text gives limited dimensions.

Show 6 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	PET substrate	not reported	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2
Other	carbon paste	printed three-electrode pattern	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2
Other	silver/silver chloride paste	coated reference electrode area	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2
Base	KOH	1.70 g	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2
Additive	PVA	10 g	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2
Solvent	deionised water	100 mL	2-3 · 2.5 Assembly of noninvasive glucose sensor · Fig. S2