Synthesis · Comparing Ag-O coordinated AgMOF-5 and Ag-N coordinated Ag nanosphere catalytic polymers for real time monitoring of H2O2 level in cancer cells

4 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

Route 1: Drop Cast

3 · 2.5. Modified electrode preparation

Solventsethanol/water 4:1 for diluted Nafion

AdditivesNafion, 0.05%

Atmosphereopen air drying

Time2 h material drying; 0.75 h Nafion drying

Substrate orientationglassy carbon electrode

Work-upprepared in the same way as AgMOF/GCE after material dispersion

Activationair dried

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Ag nanosphere	1 mg/ml used for comparison	3 · 2.5. Modified electrode preparation
Additive	Nafion solution	10 uL · 0.05%	3 · 2.5. Modified electrode preparation

Partial recipeSource: Main

Route 2: Other

3 · 2.3. Synthesis of AgMOF-derived Ag nanosphere

Metal precursorsas-synthesised dry AgMOF

Linker precursors2-methylimidazole / Hmim, 0.492 g

Solventsdistilled water; ethanol wash

AdditivesHmim ligand-exchange agent

AtmosphereAr during calcination

Temperatureroom temperature ligand exchange; 700 C calcination

Time0.167 h ligand exchange; 6 h drying; 8 h calcination

Oxidant / reductantHmim ligand exchange/reduction environment; authors describe Ag+ reduction to metallic Ag during transformation

Work-upwashed three times with DI water, followed by ethanol; dried in a hot air oven for 6 h

Activationcalcined 8 h at 700 C in Ar

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	AgMOF solution	2.0 mmol in 120 ml distilled water	3 · 2.3. Synthesis of AgMOF-derived Ag nanosphere
Linker	Hmim solution	0.492 gm Hmim in 120 ml	3 · 2.3. Synthesis of AgMOF-derived Ag nanosphere
Solvent	distilled water	120 ml + 120 ml	3 · 2.3. Synthesis of AgMOF-derived Ag nanosphere

Complete recipeSource: Main

Route 3: Drop Cast

3 · 2.5. Modified electrode preparation

Solventsethanol/water 4:1 for diluted Nafion

AdditivesNafion, 0.05%

Atmosphereopen air drying

Time2 h material drying; 0.75 h Nafion drying

Substrate orientationglassy carbon electrode

Work-upsonicated material solution 20 min before casting

Activationair dried

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	AgMOF	10 uL cast · 1 mg/ml	3 · 2.5. Modified electrode preparation
Additive	Nafion solution	10 uL · 0.05%	3 · 2.5. Modified electrode preparation

Complete recipeSource: Main

Route 4: Solvothermal

3 · 2.2. Synthesis of AgMOF

Metal precursorsAgNO3, 1.435 g

Linker precursorsH2BDC / terephthalic acid, 0.475 g

SolventsDMF, 40 mL

Temperature120

Time20

Work-upcentrifuged 15 min at 6000 rpm; washed thoroughly with DI water then ethanol

Activationdried at 50 C overnight

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	H2BDC	0.475 gm	3 · 2.2. Synthesis of AgMOF
Solvent	DMF	40 ml	3 · 2.2. Synthesis of AgMOF
Metal Source	AgNO3	1.435 gm	3 · 2.2. Synthesis of AgMOF