Sensing Application — Aggregation-induced enhancement of pyrene-based metal-organic framework as a new electrochemiluminescence emitter for ultrasensitive detection of sulfadimethoxine

10 measurement groups · 34 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

ECL condition optimisation

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Optimised cathodic co-reactant, PBS pH, AuNP volume, K2S2O8 concentration, SDM incubation time and tracer-label incubation time.

Geometry: aptasensor electrode
Context: full composite aptasensor
Measurement source: Experimental optimisation · Figs. S4-S5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Optimum AuNPs volume	10 uL	—	—	Text Exact Reported	Experimental optimisation · Fig. S5B
Optimum K2S2O8 concentration	0.05 M	—	—	Text Exact Reported	Experimental optimisation · Fig. S5C
Optimum PBS pH	7.0	—	—	Text Exact Reported	Experimental optimisation · Fig. S5A
Optimum SDM incubation time	1.5 h	—	—	Text Exact Reported	Experimental optimisation · Fig. S5D
Optimum tracer label incubation time	2 h	—	—	Text Exact Reported	Experimental optimisation · Fig. S5E

ECL SDM calibration

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

ECL response measured for SDM concentrations from 10.0 fg mL-1 to 100 ng mL-1; error bars n=3.

Geometry: aptasensor electrode in 0.1 M PBS, pH 7.0, containing 0.05 M K2S2O8
Context: full composite aptasensor
Measurement source: 5 · 3.6 Detection of SDM with the prepared ECL aptasensor · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Calibration correlation coefficient	R = 0.9977	—	—	Text Exact Reported	5 · 3.6 Detection of SDM with the prepared ECL aptasensor · Fig. 4B
Calibration slope	1973.70	—	—	Text Exact Reported	5 · 3.6 Detection of SDM with the prepared ECL aptasensor · Fig. 4B
SDM calibration upper bound	100 ng mL-1	—	—	Text Exact Reported	1 · Abstract
SDM calibration lower bound	10.0 fg mL-1	—	—	Text Exact Reported	1 · Abstract
SDM limit of detectionMarked as a best value within this paper	1.28 fg mL-1	—	3S/m criterion	Text Exact Reported	5 · 3.6 Detection of SDM with the prepared ECL aptasensor · Fig. 4B

Standard-addition recovery in milk and chicken-paste quality-control samples

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Milk centrifuged, stood, filtered and diluted 10 times with 0.1 M PBS pH 7.0; chicken paste extracted with ethyl acetate and diluted with PBS.

Geometry: aptasensor electrode
Context: full composite aptasensor applied to real matrices
Measurement source: 7 · 3.8 Real sample analysis · Tables S4-S5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Chicken-paste quality-control sample recovery	99.8 to 108%	—	reported range; numeric field is midpoint	Text Range	7 · 3.8 Real sample analysis · Table S5
Chicken-paste quality-control sample RSD	2.51 to 5.40%	—	reported range; numeric field is midpoint	Text Range	7 · 3.8 Real sample analysis · Table S5
Milk sample SDM recovery	97.9 to 107%	—	reported range; numeric field is midpoint	Text Range	7 · 3.8 Real sample analysis · Table S4
Milk sample RSD	1.75-4.21%	—	reported range; numeric field is midpoint	Text Range	7 · 3.8 Real sample analysis · Table S4

Intra- and inter-batch ECL repeatability

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Aptasensor constructed with SDM at 1 ng mL-1.

Geometry: aptasensor electrode
Context: full composite aptasensor
Measurement source: 6 · 3.7 Selectivity, repeatability, and stability · Fig. 5B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Inter-assay repeatability RSD	3.72%	—	—	Text Exact Reported	6 · 3.7 Selectivity, repeatability, and stability · Fig. 5B
Intra-assay repeatability RSD	4.26%	—	—	Text Exact Reported	6 · 3.7 Selectivity, repeatability, and stability · Fig. 5B

Raw ECL response table for milk samples

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Triplicate ECL intensity values for milk samples spiked with 100 ng mL-1, 10.0 ng mL-1, 1.00 ng mL-1 and 100 pg mL-1 SDM.

Geometry: aptasensor electrode
Context: full composite aptasensor in real milk matrix
Measurement source: 14 · S10 Real sample analysis · Table S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Milk raw ECL intensity at 100 ng mL-1 SDM	17571, 17633, 17583	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S2
Milk raw ECL intensity at 100 pg mL-1 SDM	11623, 11645, 11574	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S2
Milk raw ECL intensity at 10.0 ng mL-1 SDM	15548, 15532, 15564	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S2
Milk raw ECL intensity at 1.00 ng mL-1 SDM	13587, 13570, 13600	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S2

Raw ECL response table for quality-control chicken-paste samples

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Triplicate ECL intensity values for quality-control samples containing 852 pg mL-1, 8.52 pg mL-1 and 85.2 fg mL-1 SDM.

Geometry: aptasensor electrode
Context: full composite aptasensor in chicken-paste quality-control matrix
Measurement source: 14 · S10 Real sample analysis · Table S3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Quality-control raw ECL intensity at 852 pg mL-1 SDM	13490, 13468, 13400	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S3
Quality-control raw ECL intensity at 85.2 fg mL-1 SDM	5600, 5548, 5550	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S3
Quality-control raw ECL intensity at 8.52 pg mL-1 SDM	9597, 9577, 9554	—	triplicate raw values; numeric is arithmetic mean	SI Table Exact Reported	14 · S10 Real sample analysis · Table S3

Recovery and RSD tables for milk and quality-control samples

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Mean found SDM, recovery and RSD values for milk samples and quality-control chicken-paste samples.

Geometry: aptasensor electrode
Context: full composite aptasensor in real matrices
Measurement source: 15 · S10 Real sample analysis · Tables S4-S5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
milk sample 1 recovery	added 100 ng mL-1; found 107 ng mL-1; recovery 107%; RSD 3.87%	—	RSD 3.87%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S4
milk sample 4 recovery	added 100 pg mL-1; found 99.5 pg mL-1; recovery 99.5%; RSD 4.21%	—	RSD 4.21%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S4
milk sample 2 recovery	added 10.0 ng mL-1; found 9.80 ng mL-1; recovery 97.9%; RSD 1.87%	—	RSD 1.87%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S4
milk sample 3 recovery	added 1.00 ng mL-1; found 0.992 ng mL-1; recovery 99.2%; RSD 1.75%	—	RSD 1.75%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S4
quality-control sample 1 recovery	standard 852 pg mL-1; found 850 pg mL-1; recovery 99.8%; RSD 5.4%	—	RSD 5.4%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S5
quality-control sample 3 recovery	standard 85.2 fg mL-1; found 85.7 fg mL-1; recovery 101%; RSD 3.54%	—	RSD 3.54%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S5
quality-control sample 2 recovery	standard 8.52 pg mL-1; found 9.23 pg mL-1; recovery 108%; RSD 2.51%	—	RSD 2.51%	SI Table Exact Reported	15 · S10 Real sample analysis · Table S5

Interference/selectivity ECL assay

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

SDM at 10 pg mL-1; interfering substances SDZ, SDO, SMM and SMZ at 1 ng mL-1 and mixture.

Geometry: aptasensor electrode
Context: full composite aptasensor
Measurement source: 6 · 3.7 Selectivity, repeatability, and stability · Fig. 5A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Interferent concentration relative to SDM	1 ng mL-1 interferents, 100 times higher than SDM at 10 pg mL-1	—	—	Text Exact Reported	5 · 3.7 Selectivity, repeatability, and stability · Fig. 5A
Selectivity against sulfonamide interferents	interference signals did not significantly differ from blank; SDM and mixture signals comparable and much greater	—	—	Text Qualitative	6 · 3.7 Selectivity, repeatability, and stability · Fig. 5A

Room-temperature storage stability

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Prepared aptasensor analysed after three weeks storage at room temperature.

Atmosphere: room-temperature storage
Geometry: aptasensor electrode
Context: full composite aptasensor
Measurement source: 7 · 3.7 Selectivity, repeatability, and stability · Fig. 5D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Long-term ECL intensity decrease	4.07% after three weeks	—	—	Text Exact Reported	7 · 3.7 Selectivity, repeatability, and stability · Fig. 5D

Continuous cyclic-potential scan stability

SDM/MCH/dsDNA/AuNPs/Ce-MOF/GCE with ZPM/SP tracer · Electrode

Prepared sensor incubated with SDM (10 pg mL-1) and scanned for 11 subsequent cycles.

Geometry: aptasensor electrode
Context: full composite aptasensor
Measurement source: 6 · 3.7 Selectivity, repeatability, and stability · Fig. 5C

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Short-term ECL intensity decrease	3.45% after 11 scans	—	—	Text Exact Reported	6 · 3.7 Selectivity, repeatability, and stability · Fig. 5C