Synthesis · Bifunctional 3D-MOF-based nanoprobes for electrochemical sensing and nanozyme enhanced with peroxidase mimicking for colorimetric detection of acetaminophen

6 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

Route 1: Drop Cast

3 · 2.3 Fabrication of different electrodes

SolventsSample solution solvent not specified; GCE washed with DI water and ethanol

AdditivesAlumina slurry used for GCE cleaning

AtmosphereNot reported

Work-upBare GCE washed with DI water and ethanol and cleaned with alumina slurry before sample loading.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ZIF-67-C sample solution	about 6 mL as printed in text	3 · 2.3 Fabrication of different electrodes
Solvent	distilled water and ethanol	copious	3 · 2.3 Fabrication of different electrodes
Additive	alumina slurry	Not specified	3 · 2.3 Fabrication of different electrodes

Partial recipeSource: Main

Route 2: Drop Cast

3 · 2.3 Fabrication of different electrodes

SolventsSample solution solvent not specified; GCE washed with DI water and ethanol

AdditivesAlumina slurry used for GCE cleaning

AtmosphereNot reported

Work-upBare GCE washed with DI water and ethanol and cleaned with alumina slurry before sample loading.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ZIF-67-A sample solution	about 6 mL as printed in text	3 · 2.3 Fabrication of different electrodes
Solvent	distilled water and ethanol	copious	3 · 2.3 Fabrication of different electrodes
Additive	alumina slurry	Not specified	3 · 2.3 Fabrication of different electrodes

Partial recipeSource: Main

Route 3: Drop Cast

3 · 2.3 Fabrication of different electrodes

SolventsSample solution solvent not specified; GCE washed with DI water and ethanol

AdditivesAlumina slurry used for GCE cleaning

AtmosphereNot reported

Work-upBare GCE washed with DI water and ethanol and cleaned with alumina slurry before sample loading.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ZIF-67-H sample solution	about 6 mL as printed in text	3 · 2.3 Fabrication of different electrodes
Solvent	distilled water and ethanol	copious	3 · 2.3 Fabrication of different electrodes
Additive	alumina slurry	Not specified	3 · 2.3 Fabrication of different electrodes

Complete recipeSource: Both

Route 4: Other

3 · 2.2 Synthesis procedure

Metal precursors0.1 M cobalt nitrate in 25 mL methanol

Linker precursors0.1 M 2-methylimidazole in 25 mL methanol

SolventsMethanol

AtmosphereNot reported

Temperatureroom temperature; dried at 50 C

Timesame initial procedure as ZIF-67-C, followed by 12 h ageing at room temperature

Work-upPurple product washed several times with copious methanol.

ActivationDried at 50 C in a hot-air oven; no separate activation reported.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	cobalt nitrate (MW-291.02, >=98% in SI)	25 mL solution · 0.1 M	3 · 2.2 Synthesis procedure
Linker	2-methyl imidazole (2-MeIm; MW-82.10, 99% in SI)	25 mL solution · 0.1 M	3 · 2.2 Synthesis procedure
Solvent	methanol	25 mL + 25 mL	3 · 2.2 Synthesis procedure

Complete recipeSource: Both

Route 5: Other

2-3 · 2.2 Synthesis procedure

Metal precursors0.1 M cobalt nitrate in 25 mL methanol

Linker precursors0.1 M 2-methylimidazole in 25 mL methanol

SolventsMethanol

AtmosphereNot reported

Temperatureroom temperature; dried at 50 C

Time0.333 h dropwise/stirring stage plus 12 h stirring

Work-upPurple product washed several times with copious methanol to remove loosely bonded by-products.

ActivationDried at 50 C in a hot-air oven; no separate activation reported.

Scalability contextAuthors describe the method as simple co-precipitation at room temperature.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	cobalt nitrate (MW-291.02, >=98% in SI)	25 mL solution · 0.1 M	2-3 · 2.2 Synthesis procedure
Linker	2-methyl imidazole (2-MeIm; MW-82.10, 99% in SI)	25 mL solution · 0.1 M	2-3 · 2.2 Synthesis procedure
Solvent	methanol	25 mL + 25 mL	2-3 · 2.2 Synthesis procedure

Complete recipeSource: Both

Route 6: Hydrothermal

3 · 2.2 Synthesis procedure

Metal precursors0.1 M cobalt nitrate in 25 mL methanol

Linker precursors0.1 M 2-methylimidazole in 25 mL methanol

SolventsMethanol

AtmosphereNot reported

Temperature180 C; dried at 50 C

Time20 min stirring then 12 h in autoclave

Work-upPurple product washed several times with copious methanol.

ActivationDried at 50 C in a hot-air oven; no separate activation reported.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	cobalt nitrate (MW-291.02, >=98% in SI)	25 mL solution · 0.1 M	3 · 2.2 Synthesis procedure
Linker	2-methyl imidazole (2-MeIm; MW-82.10, 99% in SI)	25 mL solution · 0.1 M	3 · 2.2 Synthesis procedure
Solvent	methanol	25 mL + 25 mL	3 · 2.2 Synthesis procedure