cMOF Evidence Base
Sensing Application — Bifunctional 3D-MOF-based nanoprobes for electrochemical sensing and nanozyme enhanced with peroxidase mimicking for colorimetric detection of acetaminophen

Measurement evidence

Sensing Application

Bifunctional 3D-MOF-based nanoprobes for electrochemical sensing and nanozyme enhanced with peroxidase mimicking for colorimetric detection of acetaminophen · Nataraj N., Chen T.-W., Gan Z.-W. et al. · Materials Today Chemistry · 2022 · 100725

12 measurement groups · 54 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

UV-vis colorimetric ACM detection

UV-visible and optical absorption spectroscopy

ZIF-67-C powder · Powder

Varied ACM concentration with constant ZIF-67-C catalyst, TMB, and H2O2; absorbance at 652 nm.

Geometry
Dispersion colorimetric assay
Context
Pristine powder nanozyme application
Measurement source
9-10 · 3.3.2 Kinetic analysis of peroxidase-catalytic activity · Fig. 9A; Table 1
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Colorimetric ACM linear rangeMarked as a best value within this paper0.010-100 uM——Table
Range		8-9 · 3.3.2 Kinetic analysis of peroxidase-catalytic activity · Table 1
Colorimetric limit of detection for ACMMarked as a best value within this paper0.034 uM——Text
Exact Reported		8-9 · 3.3.2 Kinetic analysis of peroxidase-catalytic activity · Table 1
Colorimetric ACM calibration high-range R2 from SI Fig. S5AR2 = 0.9905——Visual Estimate
Approximate		6-7 · Fig. S4/S5 · Fig. S5A
Colorimetric ACM calibration low-range R2 from SI Fig. S5AR2 = 0.9976——Visual Estimate
Approximate		6-7 · Fig. S4/S5 · Fig. S5A

UV-vis colorimetric peroxidase-mimicking assay

UV-visible and optical absorption spectroscopy

ZIF-67-C powder · Powder

TMB/H2O2/ACM/buffer reaction systems; absorbance maximum at 652 nm; compared ZIF-67-C, ZIF-67-A, and ZIF-67-H.

Geometry
Dispersion colorimetric assay
Context
Pristine powder nanozyme comparison
Measurement source
9 · 3.3.1 Investigation of intrinsic peroxidase activity · Fig. 8
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Best peroxidase-mimicking ZIF-67 variantMarked as a best value within this paperZIF-67-C showed the highest absorbance spectrum compared with ZIF-67-A and ZIF-67-H.——Text
Qualitative		9 · 3.3.1 Investigation of intrinsic peroxidase activity · Fig. 8B
Oxidised TMB absorbance maximum652 nm——Text
Exact Reported		9 · 3.3.1 Investigation of intrinsic peroxidase activity · Fig. 8

Colorimetric pH/time/temperature optimisation

ZIF-67-C powder · Powder

pH range 2-6, reaction time 1-40 min, temperature 25-50 C; TMB/H2O2/ACM assay.

Geometry
Dispersion colorimetric assay
Context
Pristine powder nanozyme application
Measurement source
10 · 3.3.3 Colorimetric parameters analysis · Fig. S5 (SI text caption only)
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Colorimetric selectivity ACM absorbance visual estimateACM bar approximately 0.70 cps, interferents much lower——Visual Estimate
Approximate		6 · Fig. S4 · Fig. S4C
Optimised colorimetric assay pHMarked as a best value within this paperpH 4——Text
Exact Reported		10 · 3.3.3 Colorimetric parameters analysis · Fig. S5C (SI text caption only)
Optimised colorimetric assay temperatureMarked as a best value within this paper35 C——Text
Exact Reported		10 · 3.3.3 Colorimetric parameters analysis · Fig. S5D (SI text caption only)
Optimised colorimetric reaction timeMarked as a best value within this paper10 min——Text
Exact Reported		10 · 3.3.3 Colorimetric parameters analysis · Fig. S5B (SI text caption only)
Colorimetric stability absorbance in SI histogramapproximately 1.0 cps across 0, 5, 10, and 15 days——Visual Estimate
Approximate		6 · Fig. S4 · Fig. S4E

UV-vis steady-state kinetic assay

UV-visible and optical absorption spectroscopy

ZIF-67-C powder · Powder

Varied ACM or TMB concentration with ZIF-67-C and constant remaining assay components; Michaelis-Menten and Lineweaver-Burk analysis discussed.

Geometry
Dispersion colorimetric assay
Context
Pristine powder nanozyme application
Measurement source
6 · Fig. S4 · Fig. S4A-B
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Steady-state ACM concentration range in kinetic plot10 nM to 100 mM as labelled in Fig. 9A——Figure Axis
Approximate		10 · 3.3.2 Kinetic analysis of peroxidase-catalytic activity · Fig. 9A
Steady-state TMB concentration range in kinetic plot100 uM to 30 mM as labelled in Fig. 9B——Figure Axis
Approximate		10 · 3.3.2 Kinetic analysis of peroxidase-catalytic activity · Fig. 9B
Lineweaver-Burk R2 for ACM kinetic plotR-square (COD) 0.99531——Visual Estimate
Approximate		6-7 · Fig. S4/S5 · Fig. S4A
Lineweaver-Burk R2 for TMB kinetic plotR-square (COD) 0.99479——Visual Estimate
Approximate		6-7 · Fig. S4/S5 · Fig. S4B

Cyclic voltammetry concentration response

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

Successive ACM addition from 0 to 280 uM at 50 mV/s in pH 7 electrolyte.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
5-6 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 4A-B
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
CV concentration calibration equationy = 0.0775x - 0.7708——Text
Exact Reported		5 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 4B
CV concentration calibration R2R2 = 0.9944——Text
Exact Reported		5 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 4B
CV ACM concentration range0 to 280 uM ACM——Caption
Range		6 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 4A

Cyclic voltammetry

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

PB solution at pH 7 with 130 uM ACM; sweep rate 50 mV/s; compared ZIF-67-C/GCE, ZIF-67-A/GCE, ZIF-67-H/GCE, and bare GCE.

Geometry
Modified GCE electrodes
Context
Composite electrode application with bare and variant controls.
Measurement source
5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 3B
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Anodic peak current for ACM oxidation at bare GCE0.83 uA——Text
Rounded Reported		5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 3B
Anodic peak current for ACM oxidation at ZIF-67-A/GCE7.07 uA——Text
Rounded Reported		5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 3B
Anodic peak current for ACM oxidation at ZIF-67-C/GCEMarked as a best value within this paper9.4 uA——Text
Rounded Reported		5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 3B
Anodic peak current for ACM oxidation at ZIF-67-H/GCE4.8 uA——Text
Rounded Reported		5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 3B

Cyclic voltammetry pH study

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

pH 3 to 11; 50 mV/s scan rate; 130 uM ACM.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
6-7 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 5
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
pH-potential linear equationy = -0.082x + 0.716——Text
Exact Reported		7 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 5B
pH-potential linearity R2R2 = 0.9976——Text
Exact Reported		7 · 3.2.3 Effect of ACM over ZIF-67-C/GCE · Fig. 5B

Cyclic voltammetry scan-rate study

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

130 uM ACM in pH 7 at ZIF-67-C/GCE; scan rate varied from 20 to 200 mV/s.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
5-6 · 3.2.2 Electrochemical oxidation of ACM · Fig. 4C-D
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Scan-rate calibration R2R2 = 0.9901——Text
Exact Reported		5 · 3.2.2 Electrochemical oxidation of ACM · Fig. 4D
Scan-rate range20 to 200 mV/s——Caption
Range		6 · 3.2.2 Electrochemical oxidation of ACM · Fig. 4C

Differential pulse voltammetry

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

ACM concentration varied from 0.199 to 1098.31 uM at ZIF-67-C/GCE in pH 7.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
7-8 · 3.2.4 DPV detection of ACM · Fig. 6A-B; Table 1
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
DPV tested ACM concentration range0.199 to 1098.31 uM——Caption
Range		8 · 3.2.4 DPV detection of ACM · Fig. 6A
DPV linear range for ZIF-67-C/GCEMarked as a best value within this paper0.001-800 uM——Table
Range		8 · 3.2.4 DPV detection of ACM · Table 1
DPV limit of detection for ACMMarked as a best value within this paper0.014 uM——Text
Exact Reported		7-8 · 3.2.4 DPV detection of ACM · Table 1
DPV sensitivityMarked as a best value within this paper4.283 uM-1 A-1 cm-2——Text
Exact Reported		7 · 3.2.4 DPV detection of ACM

DPV anti-interference/selectivity test

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

Catechol, dopamine, levofloxacin, ibuprofen, uric acid, nilutamide, aspirin, ciprofloxacin, sodium ions, and flutamide injected at 20-fold higher concentrations than ACM in pH 7.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
7-8 · 3.2.5 Selectivity studies for electrochemical detection of ACM · Fig. 6C-D
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
DPV selectivity against interferentsInterferents at 20-fold higher concentration showed lower current response than ACM.——Text
Qualitative		7-8 · 3.2.5 Selectivity studies for electrochemical detection of ACM · Fig. 6C-D

Real sample electrochemical and colorimetric detection

ZIF-67-C/GCE modified electrode · Electrode

River water and lake water samples from Keelung river/lake, filtered, centrifuged 20 min at 2600 rpm, diluted with PB solution, and spiked with ACM; Table S1 gives recovery values.

Geometry
ZIF-67-C/GCE and ZIF-67-C colorimetric assay
Context
Application validation
Measurement source
4-5 · S2 Real sample pretreatment; Table S1 · Table S1
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Real sample found ACM for lake water at 120 uM addedadded 120 uM; found 118.12 uM; recovery 98.9%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for lake water at 120 uM added98.9%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for lake water at 160 uM addedadded 160 uM; found 159.38 uM; recovery 99.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for lake water at 160 uM added99.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for lake water at 200 uM addedadded 200 uM; found 197.23 uM; recovery 98.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for lake water at 200 uM added98.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for lake water at 40 uM addedadded 40 uM; found 39.45 uM; recovery 99.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for lake water at 40 uM added99.6%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for lake water at 80 uM addedadded 80 uM; found 78.56 uM; recovery 98.2%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for lake water at 80 uM added98.2%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for river water at 120 uM addedadded 120 uM; found 118.23 uM; recovery 98.5%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for river water at 120 uM added98.5%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for river water at 160 uM addedadded 160 uM; found 158.23 uM; recovery 98.8%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for river water at 160 uM added98.8%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for river water at 200 uM addedadded 200 uM; found 198.56 uM; recovery 99.2%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for river water at 200 uM added99.2%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for river water at 40 uM addedadded 40 uM; found 39.92 uM; recovery 99.8%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for river water at 40 uM added99.8%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample found ACM for river water at 80 uM addedadded 80 uM; found 79.12 uM; recovery 98.9%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1
Real sample recovery for river water at 80 uM added98.9%——SI Table
Exact Reported		4-5 · Table S1 Real sample detection · Table S1

CV reproducibility and stability

Voltammetry

ZIF-67-C/GCE modified electrode · Electrode

Five independently chosen ZIF-67-C/GCE electrodes; 130 uM ACM in pH 7 at 50 mV/s. Stability tested on day 1, day 15, and day 30 after storage at 3 C.

Geometry
ZIF-67-C/GCE
Context
Composite electrode application
Measurement source
7-9 · 3.2.6 Reproducibility, stability, and repeatability analysis · Fig. 7
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Electrochemical repeatability current in SI histogramapproximately 9-10 uA across 3 repeats——Visual Estimate
Approximate		6 · Fig. S4 · Fig. S4D
Electrochemical stability duration30 days at 3 C, tested day 1, day 15, and day 30——Text
Exact Reported		7 · 3.2.6 Reproducibility, stability, and repeatability analysis · Fig. 7B
Number of reproducibility electrodes5 different ZIF-67-C/GCE electrodes——Text
Exact Reported		7 · 3.2.6 Reproducibility, stability, and repeatability analysis · Fig. 7A