Sensing Application — ZnS/MnO2 metal organic framework based conductive hydrogel for highly selective and sensitive detection of glutathione in serum samples

6 measurement groups · 60 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Amperometry selectivity test

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

Amperometric response at +0.23 V vs Ag/AgCl for 100 nM GSH with 2-fold concentrations of AA, BSA, Ca2+, Cl-, glucose, K+, Na+ and uric acid.

Geometry: Hydrogel sensor electrode.
Context: Composite hydrogel selectivity against serum interferents.
Measurement source: p.8-p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. 6(c)

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Selectivity test GSH concentration	100 nM GSH	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. 6(c)
Interferent concentration relative to GSH	2-fold concentrations of interfering species	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. 6(c)
Selectivity amperometry potential	+0.23 V vs Ag/AgCl	—	—	Caption Exact Reported	p.8 · Figure 6 caption · Fig. 6(c)
Selectivity against serum interferents	current did not significantly change in the presence of interferents and increased only after GSH addition	—	—	Text Qualitative	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. 6(c)

Differential pulse voltammetry (DPV)

Voltammetry

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

GSH sensing in PBS electrolyte solution; concentration range 10 nM to 10 mM; potential window -0.1 to 0.5 V.

Geometry: ZnS/MnO2-MOF hydrogel sensor electrode.
Context: Composite hydrogel analytical performance.
Measurement source: p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(a,b)

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Limit of detection for GSHMarked as a best value within this paper	6.88 nM	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(b); Table 1
LOD calculation formula	LOD = 3S/m	—	—	Text Qualitative	p.8 · 3.4. Electrochemical sensing of glutathione
DPV potential window lower bound	-0.1 V	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(a)
DPV potential window upper bound	0.5 V	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(a)
GSH linear detection range lower boundMarked as a best value within this paper	10 nM	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(a,b)
GSH linear detection range upper boundMarked as a best value within this paper	10 mM	10000000 nM	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(a,b)
DPV GSH calibration intercept	0.0005	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(b)
DPV GSH calibration R2	0.996	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(b)
DPV GSH calibration slope	Y = 0.00845(X) + 0.0005	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(b)
GSH sensor sensitivityMarked as a best value within this paper	8.45 uA.nM^-1.cm^-2	—	—	Text Exact Reported	p.8 · 3.4. Electrochemical sensing of glutathione · Fig. 6(b)

DPV standard addition in simulated blood serum

Voltammetry

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

Unknown GSH measured first, then known GSH added to electrolyte solution; samples S1, S2 and S3 represent different GSH concentrations; N=5 devices in Fig. 8 caption.

Geometry: Hydrogel sensor electrode; simulated blood serum matrix.
Context: Composite hydrogel real-sample application.
Measurement source: p.9-p.10 · 3.6. Real sample analysis · Fig. 8; Table S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Simulated blood serum GSH concentration, sample S1	1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Simulated blood serum GSH concentration, sample S2	2 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Simulated blood serum GSH concentration, sample S3	3 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Number of devices in simulated blood serum calibration	N = 5 devices	—	—	Caption Exact Reported	p.10 · Figure 8 caption · Fig. 8
Simulated blood serum calibration R2	R2 = 0.998	—	—	Text Exact Reported	p.9-p.10 · 3.6. Real sample analysis · Fig. 8(b)
GSH recovered, sample S1	0.30 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
GSH recovered, sample S2	0.45 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
GSH recovered, sample S3	0.57 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Simulated blood serum recovery, sample S1	92.59 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Simulated blood serum recovery, sample S2	101.00 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Simulated blood serum recovery, sample S3	120.22 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Relative standard deviation, sample S1	5.85 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Relative standard deviation, sample S2	0.25 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Relative standard deviation, sample S3	0.35 %	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Spiked GSH concentration, sample S1	1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Spiked GSH concentration, sample S2	1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2
Spiked GSH concentration, sample S3	1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S2

Reproducibility test

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

Five independently prepared hydrogel substrates tested for detection of 50 nM GSH under ideal conditions.

Geometry: Hydrogel sensor electrodes, N=5.
Context: Composite hydrogel device-to-device reproducibility.
Measurement source: p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(a)

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Reproducibility test GSH concentration	50 nM GSH	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(a)
Number of independently prepared electrodes	five independently prepared	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(a)
Reproducibility RSD	about 4.1 %	—	about	Text Approximate	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(a)
Estimated current for independent electrode 1	~0.0029 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(a)
Estimated current for independent electrode 2	~0.0028 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(a)
Estimated current for independent electrode 3	~0.0029 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(a)
Estimated current for independent electrode 4	~0.0027 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(a)
Estimated current for independent electrode 5	~0.0028 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(a)

Supplementary analytical-performance comparison table

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

SI Table S1 compares glutathione determination methods by detection limit and linear range; literature comparator rows are contextual, while the electrochemical row is this work.

Context: Benchmark comparison for the composite hydrogel sensor.
Measurement source: p.3 · Supplementary Information · Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Table S1 Electrochemical, this work detection limitMarked as a best value within this paper	0.00688 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Electrochemical, this work linear range lower bound	0.01-10000 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Electrochemical, this work linear range upper boundMarked as a best value within this paper	0.01-10000 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Electrochemiluminescence detection limit	0.05 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Electrochemiluminescence linear range lower bound	0.1-1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Electrochemiluminescence linear range upper bound	0.1-1 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Fluorescence detection limit	0.06 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Fluorescence linear range lower bound	0-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Fluorescence linear range upper bound	0-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 HPLC detection limit	0.5 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 HPLC linear range lower bound	0.75-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 HPLC linear range upper bound	0.75-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Photoelectrochemical detection limit	0.01 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Photoelectrochemical linear range lower bound	0.01-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1
Table S1 Photoelectrochemical linear range upper bound	0.01-10 uM	—	—	SI Table Exact Reported	p.3 · Supplementary Information · Table S1

Storage stability test

2 wt% ZnS/MnO2-MOF hydrogel electrode · Electrode

Hydrogel substrate stored at 4 C for 14 days, then tested for 50 nM GSH under optimal conditions.

Temperature: 277.15
Geometry: Hydrogel sensor electrode.
Context: Composite hydrogel shelf-life/stability.
Measurement source: p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(b)

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
After-14-days CV anodic peak current	~0.00175 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(b)
First-day CV anodic peak currentMarked as a best value within this paper	~0.00215 mA	—	—	Figure Axis Approximate	p.2 · Supplementary Information · Figure S3(b)
Storage duration	14 days	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(b)
Retained response after 14 daysMarked as a best value within this paper	98.5 % of its initial response	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(b)
Stability RSD	3.90 %	—	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(b)
Storage temperature	4 C	277.15 K	—	Text Exact Reported	p.9 · 3.5. Reproducibility, stability and selectivity studies · Fig. S3(b)