Synthesis · ZnS/MnO2 metal organic framework based conductive hydrogel for highly selective and sensitive detection of glutathione in serum samples

3 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

p.2 · 2.2. Hydrothermal synthesis of ZnS · Fig. 1(a)

Metal precursorsZinc acetate dihydrate, Zn(CH3COO)2.2H2O.

Solvents20 ml deionized water; ethanol used in washing.

AdditivesThiourea, SC(NH2)2, used as sulfur source.

Atmospherenot stated

Temperature180; drying at 80

Time20; drying 10

Work-upCool to room temperature; wash repeatedly with deionized water and ethanol; centrifuge at 4000 rpm for 20 min.

ActivationHot-air oven drying at 80 C for 10 h.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	zinc acetate dihydrate (Zn(CH3COO)2.2H2O; 99 %)	ratio 2:1 versus thiourea	p.2 · 2.2. Hydrothermal synthesis of ZnS · Fig. 1(a)
Other	thiourea (SC(NH2)2; 99 %)	ratio 1:2 versus zinc acetate	p.2 · 2.2. Hydrothermal synthesis of ZnS · Fig. 1(a)
Solvent	deionized water	20 ml	p.2 · 2.2. Hydrothermal synthesis of ZnS · Fig. 1(a)
Solvent	ethanol	used for washing	p.2 · 2.2. Hydrothermal synthesis of ZnS · Fig. 1(a)

Partial recipeSource: Main

p.3 · 2.4. Synthesis of ZnS/MnO2 - MOF hydrogel · Fig. 1(c)

Metal precursors0.2 g ZnS/MnO2-MOF.

Solvents10 ml DI water.

Additives1 g polyvinyl alcohol; 5% Nafion solution.

Atmospherenot stated

Timeovernight stirring; 10 min after Nafion addition; 24 h lyophilization

Substrate orientationTransferred to Petri dish before lyophilization.

Work-upMagnetic stirring until transparent PVA solution; add MOF and stir overnight; add 5% Nafion and stir 10 min; transfer to Petri dish.

ActivationLyophilization for 24 h.

Scalability contextConclusion states the hydrogel is cost-effective and easily scalable, but no batch-scale data are reported.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Additive	polyvinyl alcohol (average molecular weight ~89,000)	1 g	p.3 · 2.4. Synthesis of ZnS/MnO2 - MOF hydrogel · Fig. 1(c)
Solvent	DI water	10 ml	p.3 · 2.4. Synthesis of ZnS/MnO2 - MOF hydrogel · Fig. 1(c)
Other	ZnS/MnO2-MOF	0.2 g · 2 wt%	p.3 · 2.4. Synthesis of ZnS/MnO2 - MOF hydrogel · Fig. 1(c)
Additive	nafion	5% solution	p.3 · 2.4. Synthesis of ZnS/MnO2 - MOF hydrogel · Fig. 1(c)

Complete recipeSource: Main

p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)

Metal precursors0.5 g ZnS and 0.5 g MnO2.

Linker precursors0.25 g trimesic acid.

Solvents32 ml DMF, 2 ml ethanol and 2 ml deionized water.

Atmospherenot stated

Temperature180; drying at 80

Time20; dried overnight

Work-upMagnetic stirring until a transparent scarlet solution; cool autoclave naturally; wash precipitated solution multiple times with ethanol.

ActivationDried overnight at 80 C.

Show 6 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	ZnS	0.5 g · ratio 1	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)
Metal Source	manganese dioxide (MnO2; 99.09 %)	0.5 g · ratio 1	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)
Linker	trimesic acid (C9H6O6; 98 %)	0.25 g · ratio 0.5	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)
Solvent	N,N-dimethylformamide (DMF; 99.09 %)	32 ml	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)
Solvent	ethanol	2 ml plus washing	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)
Solvent	deionized water	2 ml	p.2 · 2.3. Solvothermal synthesis of ZnS/MnO2 - MOF · Fig. 1(b)