Wang Y., Mao Z., Chen Q. et al. · Biosensors and Bioelectronics · 2022 · 113954
Open a family to keep every result attached to its sample, method and conditions.
Paraphrased for this database from the authors’ stated interpretations — never quoted verbatim — and kept separate from reported measurements.
The Cu-TCPP 2D MOF/peptide SPR sensor detects PD-L1 exosomes with LOD 16.7 particles/mL and good serum recoveries.
Caveat: Application performance is for a peptide-functionalized MOF/gold composite, not pristine MOF alone.
11 · 4. Conclusion · Linked to 12 structured results
A 10 ug/mL 2D MOF deposition concentration gave the maximum RI sensitivity and was selected for subsequent PD-L1 exosome sensing.
Caveat: Intermediate concentration values other than 10 ug/mL were read from the rendered bar labels.
6 · 3.3. SPR sensitivity analysis · Fig. 5F · Linked to 5 structured results
The supplied SI text contains only supplemental sensorgram captions and does not add synthesis or tabulated measurement details.
Caveat: If original SI images are later required for exact Fig. S1-S3 traces, they should be acquired in a later batch.
Supplementary information · Figs. S1-S3
Cu-TCPP 2D MOF formation is assigned from UV-vis Q-band reduction, disappearance of TCPP OH/C=O features, new CO-O-Cu FTIR peaks, and EDS detection of Cu.
Caveat: No CIF or crystallographic refinement is supplied for this specific sample in the assigned documents.
5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3 · Linked to 7 structured results
Depositing Cu-TCPP 2D MOF on gold increases RI sensitivity, detection accuracy, and FOM relative to bare gold.
Caveat: SPR angle unit is inconsistently rendered as deg in tables and mdeg in text for some angle shifts.
7 · 3.3. SPR sensitivity analysis · Tables 1-2 · Linked to 8 structured results
The pi-stacked electroactive porphyrin molecules in TCPP-based 2D MOF carry charge transport in the MOF structure and contribute good electron mobility.
Caveat: The paper reports qualitative electrochemical evidence only; no absolute conductivity or mobility value is provided.
2 · Introduction · Linked to 2 structured results
Names and aliases are kept exactly within the paper’s own identity model.
| Material | Composition | Structure context | Source |
|---|---|---|---|
| Bare gold SPR chip/electrode | Au | unknown · Model SystemPlanar bare gold chip/electrode used as the SPR and electrochemical control. | 3 · 2.4. Construction and characterization |
| Cu-TCPP 2D MOF | Cu-TCPPCu2+ / copper-carboxylate nodes · tetra(4-carboxyphenyl)porphine (TCPP) | 2D · PristineUltrathin lamellar TCPP-based two-dimensional metalloporphyrinic MOF nanosheets; coordination bond CO-O-Cu assigned by FTIR. | 1 · Abstract |
| PD-L1 exosomes from A549 NSCLC cell culture | Not specified | unknown · Model SystemBiological vesicle analyte isolated from A549 non-small-cell lung-cancer cell culture supernatant; characterised by TEM, AFM and NTA. | 3 · 2.2. Isolation and characterization of exosomes |
Sample form, processing state and composition status define the context for measurements.
| Sample | Form and role | Processing and geometry | Source |
|---|---|---|---|
| 2D MOF-chipresearch_0372__mat__mat_cu_tcpp_2d_mof | Thin Film · Composite Sample · Composite | 50 uL 2D MOF nanosheet solution deposited on treated gold chip, incubated 30 min, rinsed, and dried with nitrogen.gold SPR chip · about 10 nm MOF layer by AFM | 3 · 2.4. Construction and characterization |
| 2D MOF/peptide SPR sensing interfaceresearch_0372__mat__mat_cu_tcpp_2d_mof | Thin Film · Composite Sample · Composite | 2D MOF-chip modified with 100 ug/mL multifunctional peptide probe before PD-L1 exosome capture.gold SPR chip | 3 · 2.4. Construction and characterization |
| Bare gold SPR chipresearch_0372__mat__mat_bare_gold_control | Thin Film · Pristine Control · Model | Rinsed with ethanol and deionized water, dried with nitrogen, and cleaned in mild piranha solution.SPR gold chip | 3 · 2.4. Construction and characterization |
| Cu-TCPP 2D MOF nanosheetsresearch_0372__mat__mat_cu_tcpp_2d_mof | Nanosheet · Target Sample · Pristine Framework | Reddish purple Cu-TCPP 2D MOF product redissolved in ethanol and stored at 4 C.about 10 nm on gold after subtracting 5 nm gold roughness | 3 · 2.3. Synthesis of Cu-TCPP 2D MOF |
| 2D MOF-modified gold electroderesearch_0372__mat__mat_cu_tcpp_2d_mof | Electrode · Composite Sample · Composite | Gold electrode polished, sonicated, piranha-cleaned, electrochemically activated in H2SO4, then modified as for SPR sensor.gold electrode | 3 · 2.4. Construction and characterization |
| Extracted PD-L1 exosomesresearch_0372__mat__mat_pd_l1_exosomes | Unknown · Target Sample · Unknown | Cell culture supernatant stored at -80 C, thawed, centrifuged at 3000 XG and 4 C for 15 min, extracted with an exosome kit, eluate collected, and exosomes stored at -80 C. | 3 · 2.2. Isolation and characterization of exosomes |