Microscopy Morphology — Rapid and sensitive detection of PD-L1 exosomes using Cu-TCPP 2D MOF as a SPR sensitizer

3 measurement groups · 12 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

AFM

Atomic force microscopy

2D MOF-chip · Thin Film

AFM of bare gold chip, 2D MOF-chip, and exosome-capturing interface.

Geometry: planar SPR gold chip
Context: composite MOF-on-gold compared with bare gold control
Measurement source: 6 · 3.2. Synthesis and characterization of 2D MOF · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Bare gold roughness	5 nm	—	—	Text Rounded Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 4A
Captured exosome size on 2D MOF	120 nm	—	—	Text Rounded Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 4C
MOF layer thickness on gold	about 10 nm	—	—	Text Approximate	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 4B

TEM, AFM, and nanoparticle tracking analysis (NTA)

Transmission electron microscopy Atomic force microscopy

Extracted PD-L1 exosomes · Unknown

Extracted A549-cell exosomes stained with uranyl acetate for TEM; surface morphology observed by AFM on smooth mica flakes; size distribution and concentration measured by NTA.

Context: biological analyte characterisation before SPR sensing
Measurement source: 4 · 3.1. Characterization of exosomes · Fig. 2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
AFM-observed exosome particle size	about 170 nm	—	—	Text Approximate	4 · 3.1. Characterization of exosomes · Fig. 2C
Extracted exosome average diameter	180 nm	—	—	Text Rounded Reported	4 · 3.1. Characterization of exosomes · Fig. 2
Extracted exosome size range	50-200 nm	—	—	Text Range	4 · 3.1. Characterization of exosomes · Fig. 2
Extracted exosome solution concentration	7.8 x 10^8 particles/mL	—	—	Text Exact Reported	4 · 3.1. Characterization of exosomes · Fig. 2

SEM, TEM, and EDS

Scanning electron microscopy Transmission electron microscopy Elemental microanalysis and mapping

Cu-TCPP 2D MOF nanosheets · Nanosheet

Morphology and elemental analysis of synthesized Cu-TCPP 2D MOF nanosheets.

Context: pristine MOF
Measurement source: 5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3C-E

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
EDS carbon fraction	68.76%	—	—	Text Exact Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3D
EDS copper fraction	4.53%	—	—	Text Exact Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3D
EDS nitrogen fraction	8.85%	—	—	Text Exact Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3D
EDS oxygen fraction	17.87%	—	—	Text Exact Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3D
MOF nanosheet lateral length	1 um	1000 nm	—	Text Rounded Reported	5 · 3.2. Synthesis and characterization of 2D MOF · Fig. 3C,E