Sensing Application — Rapid and sensitive detection of PD-L1 exosomes using Cu-TCPP 2D MOF as a SPR sensitizer

7 measurement groups · 44 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Spiked human serum recovery

2D MOF/peptide SPR sensing interface · Thin Film

PD-L1 exosomes spiked into healthy human serum samples; PBS diluted 1000 times; n=3.

Geometry: 2D MOF/peptide SPR chip
Context: application sensor in serum matrix
Measurement source: 10 · 3.4. Sensitive detection of PD-L1 exosomes · Table 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Serum recovery at 5e4 particles/mL	101.31%	—	RSD 8.96%	Table Exact Reported	11 · Table 4 · Table 4
Serum recovery at 5e5 particles/mL	102.35%	—	RSD 14.60%	Table Exact Reported	11 · Table 4 · Table 4
Serum recovery at 5e6 particles/mL	93.43%	—	RSD 5.79%	Table Exact Reported	11 · Table 4 · Table 4
Serum RSD at 5e4 particles/mL	8.96%	—	—	Table Exact Reported	11 · Table 4 · Table 4
Serum RSD at 5e5 particles/mL	14.60%	—	—	Table Exact Reported	11 · Table 4 · Table 4
Serum RSD at 5e6 particles/mL	5.79%	—	—	Table Exact Reported	11 · Table 4 · Table 4

SPR BSA antifouling assay

2D MOF/peptide SPR sensing interface · Thin Film

BSA at 10-500 ng/mL passed through unmodified and peptide-modified 2D MOF chip surfaces.

Geometry: 2D MOF and 2D MOF/peptide SPR chips
Context: peptide-modified versus unmodified 2D MOF chip
Measurement source: 10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 7C-E; Figs. S2-S3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
BSA response with peptide-modified chipMarked as a best value within this paper	0.017 deg at 500 ng/mL BSA	17 mdeg	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 7E
BSA response without peptide modification	0.105 deg at 500 ng/mL BSA	105 mdeg	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 7E

SPR PD-L1 exosome calibration and detection limit

2D MOF/peptide SPR sensing interface · Thin Film

PD-L1 exosomes from 1e4 to 5e6 particles/mL flowed through sensor; signal fitted versus log concentration.

Geometry: 2D MOF/peptide SPR chip
Context: application sensor
Measurement source: 10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8C-D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
PD-L1 exosome calibration upper bound	5 x 10^6 particles/mL	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8C-D
PD-L1 exosome calibration lower bound	10^4 particles/mL	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8C-D
Exosome calibration intercept	-0.11984 deg	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8D
Exosome calibration slope	Delta theta = 0.0324 lgCExo - 0.11984	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8D
PD-L1 exosome detection limitMarked as a best value within this paper	16.7 particles/mL	—	S/N = 3	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8D
Exosome calibration correlation coefficient R2	0.989	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8D

SPR peptide/exosome feasibility comparison

2D MOF/peptide SPR sensing interface · Thin Film

Bare gold chip and 2D MOF-chip sequentially exposed to peptides and PD-L1 exosomes under identical conditions.

Geometry: SPR gold chip with peptide-functionalized Cu-TCPP 2D MOF
Context: 2D MOF/peptide sensor versus bare gold control
Measurement source: 9 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 6C-D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
PD-L1 exosome SPR angle change on bare gold	almost did not cause a SPR angle change	—	—	Qualitative Qualitative	9 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 6D
PD-L1 exosome SPR angle change on MOF sensorMarked as a best value within this paper	50.56 mdeg	0.05056 deg	—	Text Exact Reported	9 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 6D

SPR peptide concentration optimisation

2D MOF/peptide SPR sensing interface · Thin Film

Peptides at 25, 50, 100, 150, and 200 ug/mL flowed over 2D MOF modified gold chip.

Geometry: 2D MOF-modified SPR gold chip
Context: composite peptide/MOF sensor
Measurement source: Supplementary information · Fig. S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Selected peptide concentrationMarked as a best value within this paper	100 ug/mL	—	—	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 7A-B; Fig. S1

SPR refractive-index sensitivity using NaCl RI standards

2D MOF-chip · Thin Film

RI standards 1.33330-1.36370 flowed over bare gold and 2D MOF-chip; 670 nm MP-SPR instrument.

Geometry: gold SPR chip with 2D MOF sensitizer
Context: 2D MOF-chip compared against bare gold control
Measurement source: 5 · 3.3. SPR sensitivity analysis · Fig. 5; Tables 1-2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
RI sensitivity at 20 ug/mL MOF	104.77 deg/RIU	—	—	Figure Axis Rounded Reported	7 · 3.3. SPR sensitivity analysis · Fig. 5F
RI sensitivity at 40 ug/mL MOF	99.50 deg/RIU	—	—	Figure Axis Rounded Reported	7 · 3.3. SPR sensitivity analysis · Fig. 5F
RI sensitivity at 5 ug/mL MOF	101.33 deg/RIU	—	—	Figure Axis Rounded Reported	7 · 3.3. SPR sensitivity analysis · Fig. 5F
RI sensitivity at 7.5 ug/mL MOF	129.83 deg/RIU	—	—	Figure Axis Rounded Reported	7 · 3.3. SPR sensitivity analysis · Fig. 5F
Absolute RI sensitivity increase after MOF modificationMarked as a best value within this paper	39.57 deg/RIU	—	—	Text Exact Reported	6 · 3.3. SPR sensitivity analysis
Relative RI sensitivity increase after MOF modificationMarked as a best value within this paper	40.48%	—	—	Text Exact Reported	6 · 3.3. SPR sensitivity analysis
Refractive-index standard range used for SPR sensitivity	RI = 1.33330, 1.33500, 1.33675, 1.34170, 1.34986, 1.36370	—	—	Text Exact Reported	3 · 2.5. Performance analysis of SPR sensor
Bare gold resonance angle shift	3.03 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
Bare gold refractive index change	0.03 RIU	—	—	Table Rounded Reported	7 · 3.3. SPR sensitivity analysis · Table 1
Bare gold FWHM	4.79 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
Bare gold RI sensitivity	98.00 deg/RIU	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
Bare gold Gaussian w	4.07 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
MOF-chip resonance angle shiftMarked as a best value within this paper	4.30 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
MOF-chip refractive index change	0.03 RIU	—	—	Table Rounded Reported	7 · 3.3. SPR sensitivity analysis · Table 1
MOF-chip FWHM	5.54 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
MOF-chip RI sensitivityMarked as a best value within this paper	137.67 deg/RIU	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
MOF-chip Gaussian w	4.71 deg	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 1
Bare gold detection accuracy	0.63	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 2
Bare gold figure of merit	20.42 RIU-1	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 2
MOF-chip detection accuracyMarked as a best value within this paper	0.77	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 2
MOF-chip figure of meritMarked as a best value within this paper	24.81 RIU-1	—	—	Table Exact Reported	7 · 3.3. SPR sensitivity analysis · Table 2

SPR selectivity against blood interferents

2D MOF/peptide SPR sensing interface · Thin Film

AA, GSH, glucose, BSA, and PD-L1 exosomes flowed through peptide-functionalized sensor; responses compared.

Geometry: 2D MOF/peptide SPR chip
Context: application sensor
Measurement source: 10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8A-B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Ascorbic acid SPR response	7 mdeg	0.007 deg	—	Text Rounded Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B
BSA SPR response	15 mdeg	0.015 deg	—	Text Rounded Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B
PD-L1 exosome selectivity responseMarked as a best value within this paper	97 mdeg	0.097 deg	—	Text Rounded Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B
Glucose SPR response	8 mdeg	0.008 deg	—	Text Rounded Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B
Glutathione SPR response	-1 mdeg	-0.001 deg	—	Text Rounded Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B
Selectivity significance p-value	p < 0.01	—	<	Text Exact Reported	10 · 3.4. Sensitive detection of PD-L1 exosomes · Fig. 8B