Sensing Application — Novel electrochemical sensing strategy for ultrasensitive detection of tetracycline based on porphyrin/metal phthalocyanine-covalent organic framework

5 measurement groups · 42 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

VP-ITC binding-affinity measurement for aptamer and tetracycline

Apt/CuTAPc-TFPP-COF/GCE · Electrode

50 uM TC titrated into 0.5 uM aptamer at 25 deg C; single-site binding model.

Temperature: 298
Context: aptamer-target binding, supporting sensor selectivity
Measurement source: SI text · S4. Dissociation constant of the aptamer toward TC · Fig. S4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
association constant Ka	9.26x10^5 mM	—	—	Text Exact Reported	SI text · S4. Dissociation constant of the aptamer toward TC · Fig. S4
apparent dissociation constant Kd	1.08 nM	1.08 nM	—	Text Exact Reported	SI text · S4. Dissociation constant of the aptamer toward TC · Fig. S4

EIS calibration for tetracycline detection

Impedance spectroscopy

TC/Apt/CuTAPc-TFPP-COF/GCE · Electrode

Aptasensor incubated in TC solutions at 0, 0.1, 1.0, 10, 100, 1000, 10000, and 100000 pM; Delta Rct plotted against log C_TC.

Temperature: ambient
Atmosphere: ambient
Geometry: three-electrode cell
Context: TC-bound aptasensor
Measurement source: PDF p8 · 3.5. Sensitivity of the CuTAPc-TFPP-COF-based aptasensor toward TC · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Delta Rct calibration intercept	1.49 kOhm	—	—	Caption Exact Reported	PDF p6 · Fig. 5 caption · Fig. 5c
calibration correlation coefficient R2	R2 = 0.996	—	—	Caption Exact Reported	PDF p6 · Fig. 5 caption · Fig. 5c
Delta Rct calibration slope	0.19 kOhm per log CTC(nM)	—	—	Caption Exact Reported	PDF p6 · Fig. 5 caption · Fig. 5c
TC linear/detection concentration range upper bound	100000 pM	100 nM	—	Text Exact Reported	PDF p8 · 3.5. Sensitivity · Fig. 5a
TC linear/detection concentration range lower bound	0.1 pM	0.0001 nM	—	Text Exact Reported	PDF p8 · 3.5. Sensitivity · Fig. 5a
limit of detection for tetracyclineMarked as a best value within this paper	59.6 fM	0.0596 pM	—	Text Exact Reported	PDF p8 · 3.5. Sensitivity · Fig. 5
SI Table S2 this-work EIS range and LOD summaryMarked as a best value within this paper	0.1 pM-10 nM range; LOD 59.6 fM	—	—	SI Table Exact Reported	PDF p16 · S6. Sensitivity of the CuTAPc-TFPP-COF-based aptasensor toward TC · Table S2

EIS recovery tests in spiked real samples

Impedance spectroscopy

TC/Apt/CuTAPc-TFPP-COF/GCE · Electrode

Pre-treated river water, milk, and pork samples spiked with TC at 0.1, 1.0, 10, 100, 1000, 10000, and 100000 pM and analysed with the CuTAPc-TFPP-COF aptasensor.

Temperature: ambient
Atmosphere: ambient
Geometry: three-electrode cell
Context: TC-bound aptasensor in real samples
Measurement source: PDF p7 · 3.7. Real sample analysis · Tables 1-3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
milk recovery at 0.1 pM added TC	added 0.1 pM; found 0.0962 pM; recovery 96.2%; RSD 2.35%	—	RSD 2.35%; found 0.0962 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 1.0 pM added TC	added 1.0 pM; found 0.9728 pM; recovery 97.3%; RSD 1.94%	—	RSD 1.94%; found 0.9728 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 10 pM added TC	added 10 pM; found 9.8124 pM; recovery 98.1%; RSD 2.67%	—	RSD 2.67%; found 9.8124 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 100 pM added TC	added 100 pM; found 98.619 pM; recovery 98.6%; RSD 2.18%	—	RSD 2.18%; found 98.619 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 1000 pM added TC	added 1000 pM; found 1005.384 pM; recovery 100.5%; RSD 3.24%	—	RSD 3.24%; found 1005.384 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 10000 pM added TC	added 10000 pM; found 9846.57 pM; recovery 98.5%; RSD 2.96%	—	RSD 2.96%; found 9846.57 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
milk recovery at 100000 pM added TC	added 100000 pM; found 100635.8 pM; recovery 100.6%; RSD 4.19%	—	RSD 4.19%; found 100635.8 pM	Table Exact Reported	PDF p7 · Table 2 · Table 2
pork recovery at 0.1 pM added TC	added 0.1 pM; found 0.0951 pM; recovery 95.1%; RSD 2.51%	—	RSD 2.51%; found 0.0951 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 1.0 pM added TC	added 1.0 pM; found 0.9536 pM; recovery 95.4%; RSD 2.46%	—	RSD 2.46%; found 0.9536 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 10 pM added TC	added 10 pM; found 9.8264 pM; recovery 98.3%; RSD 2.32%	—	RSD 2.32%; found 9.8264 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 100 pM added TC	added 100 pM; found 97.237 pM; recovery 97.2%; RSD 2.79%	—	RSD 2.79%; found 97.237 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 1000 pM added TC	added 1000 pM; found 1008.835 pM; recovery 100.9%; RSD 3.81%	—	RSD 3.81%; found 1008.835 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 10000 pM added TC	added 10000 pM; found 9852.47 pM; recovery 98.5%; RSD 3.29%	—	RSD 3.29%; found 9852.47 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
pork recovery at 100000 pM added TC	added 100000 pM; found 100841.9 pM; recovery 100.8%; RSD 4.54%	—	RSD 4.54%; found 100841.9 pM	Table Exact Reported	PDF p7 · Table 3 · Table 3
overall real-sample recovery range	95.1 %-100.9 %	—	RSD range 1.65 %-4.54 %	Text Range	PDF p8 · 3.7. Real sample analysis · Tables 1-3
overall real-sample RSD range	1.65 %-4.54 %	—	—	Text Range	PDF p8 · 3.7. Real sample analysis · Tables 1-3
river water recovery at 0.1 pM added TC	added 0.1 pM; found 0.0958 pM; recovery 95.8%; RSD 2.11%	—	RSD 2.11%; found 0.0958 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 1.0 pM added TC	added 1.0 pM; found 0.9594 pM; recovery 95.9%; RSD 2.13%	—	RSD 2.13%; found 0.9594 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 10 pM added TC	added 10 pM; found 9.7075 pM; recovery 97.1%; RSD 1.73%	—	RSD 1.73%; found 9.7075 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 100 pM added TC	added 100 pM; found 96.364 pM; recovery 96.4%; RSD 1.87%	—	RSD 1.87%; found 96.364 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 1000 pM added TC	added 1000 pM; found 1009.416 pM; recovery 100.9%; RSD 3.74%	—	RSD 3.74%; found 1009.416 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 10000 pM added TC	added 10000 pM; found 9704.36 pM; recovery 97.0%; RSD 1.65%	—	RSD 1.65%; found 9704.36 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1
river water recovery at 100000 pM added TC	added 100000 pM; found 100726.6 pM; recovery 100.7%; RSD 4.43%	—	RSD 4.43%; found 100726.6 pM	Table Exact Reported	PDF p7 · Table 1 · Table 1

EIS selectivity, stability, reproducibility and regenerability tests

Impedance spectroscopy

TC/Apt/CuTAPc-TFPP-COF/GCE · Electrode

Interferents at 10 pM with TC at 0.1 pM; daily stability over 15 days; five independently prepared electrodes; regeneration by 50 mM NaOH for 60 s followed by phosphate-buffer rinse and TC re-incubation.

Temperature: ambient
Atmosphere: ambient
Geometry: three-electrode cell
Context: TC-bound aptasensor
Measurement source: PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
detection efficiency preserved after seven regeneration cycles	103.2 %	—	—	Text Exact Reported	PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6d
reproducibility RSD for five electrodes	3.2 %	—	—	Text Exact Reported	PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6c
interferent concentration in selectivity test	10 pM	0.01 nM	—	Text Exact Reported	SI text · S1.5 Electrochemical measurements
TC concentration in selectivity test	0.1 pM	0.0001 nM	—	Text Exact Reported	PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6a
electrochemical signal retention after 15 days	almost 102 %	—	RSD 0.36%	Text Approximate	PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6b
stability RSD for TC detection	0.36 %	—	—	Text Exact Reported	PDF p8 · 3.6. Selectivity, stability, reproducibility, and regenerability · Fig. 6b

EIS optimisation of CuTAPc-TFPP-COF amount, aptamer concentration, incubation time and pH

Impedance spectroscopy

TC/Apt/CuTAPc-TFPP-COF/GCE · Electrode

Parameter optimisation before sensitivity testing; Fig. S5 measured Delta Rct/Rct trends under 5.0 mM [Fe(CN)6]3-/4- in phosphate buffer with NaCl and KCl.

Temperature: ambient
Atmosphere: ambient
Geometry: three-electrode cell
Context: TC-bound aptasensor optimisation
Measurement source: PDF pp13-15 · S5. Optimization of parameters for detecting TC · Fig. S5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
selected aptamer concentration	100 nM	—	—	Text Exact Reported	PDF pp13-14 · S5. Optimization of parameters for detecting TC · Fig. S5
selected aptamer incubation time	1 h	—	—	Text Exact Reported	PDF pp13-14 · S5. Optimization of parameters for detecting TC · Fig. S5
selected CuTAPc-TFPP-COF dispersion concentration	1 mg mL-1	—	—	Text Exact Reported	PDF pp13-14 · S5. Optimization of parameters for detecting TC · Fig. S5
selected detection pH	pH 7.4	—	—	Text Exact Reported	PDF pp13-14 · S5. Optimization of parameters for detecting TC · Fig. S5