Electrochemistry Application — Sensitive detection of carcinoembryonic antigen (CEA) by a sandwich-type electrochemical immunosensor using MOF-Ce@HA/Ag-HRP-Ab2 as a nanoprobe

4 measurement groups · 6 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

cyclic voltammetry

Voltammetry

sandwich CEA immunosensor on GCE · Electrode

CV from -0.2 to 0.6 V at 0.1 V s-1 in 5 mM [Fe(CN)6]3-/4- containing 0.1 mol l-1 KCl for Au/GCE, Au/Ab1/GCE, bare GCE, Au/Ab1/BSA/GCE, Au/Ab1/BSA/CEA/GCE and Au/Ab1/BSA/CEA/Ab2/GCE.

Geometry: layered GCE immunosensor
Context: electrode assembly control series
Measurement source: 6-7 · 3.2.2 · Figure 3B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CV peak-current trend during immunosensor assembly	Peak current decreased sequentially after Ab1, BSA, CEA and MoF-Ce@HA/Ag-HRP-Ab2 modification	—	—	Qualitative Qualitative	7 · 3.2.2 · Figure 3B

cyclic voltammetry and Randles-Sevcik analysis

Voltammetry

bare GCE control · Electrode

Peak current measured at scan rates 0.05, 0.1, 0.15, 0.2 and 0.25 V s-1 in K3Fe(CN)6 electrolyte; calculated effective working area.

Temperature: 298
Geometry: 4 mm glassy carbon electrode
Context: bare GCE control before MOF-containing sensor assembly
Measurement source: 6-7 · 3.2.1 · Figure 3A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Randles-Sevcik regression slope K	K = 3.55 x 10-4	—	—	Text Rounded Reported	7 · 3.2.1 · Figure 3A
GCE effective working area	0.106 cm2	—	—	Text Exact Reported	7 · 3.2.1 · Figure 3A
GCE effective area as percentage of ideal	84.4% of the ideal working area	—	—	Text Exact Reported	7 · 3.2.1 · Figure 3A

electrochemical impedance spectroscopy (EIS)

Impedance spectroscopy

sandwich CEA immunosensor on GCE · Electrode

EIS in 5 mM [Fe(CN)6]3-/4- containing 0.1 mol l-1 KCl for the same electrode assembly sequence as CV.

Geometry: layered GCE immunosensor
Context: electrode assembly control series
Measurement source: 6-7 · 3.2.2 · Figure 3C

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
EIS charge-transfer resistance trend	Au NPs reduced high-frequency semicircle radius versus bare GCE; Ab1, BSA, CEA and nanoprobe modification increased Ret stepwise	—	—	Qualitative Qualitative	7 · 3.2.2 · Figure 3C

amperometric i-t response to H2O2

Chronoamperometry

Ce-MoF@HA/Ag-HRP modified GCE · Electrode

Recorded at -0.35 V in PBS pH 7.0; 5 mM H2O2 additions at 50, 100 and 150 s; bare GCE, Au NPs, Ce-MoF@HA, Ce-MoF@HA/Ag and Ce-MoF@HA/Ag-HRP modified GCEs, all material concentrations 1.0 mg ml-1.

Geometry: modified GCE comparison electrodes
Context: component-level catalytic comparison
Measurement source: 7-8 · 3.3 · Figure 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
H2O2 amperometric catalytic response orderMarked as a best value within this paper	bare GCE no catalytic effect; Ce-MoF@HA too small; Ce-MoF@HA/Ag increased significantly; Ce-MoF@HA/Ag-HRP highest	—	—	Qualitative Qualitative	8 · 3.3 · Figure 4