Deng S., Hao Y., Yang L. et al. · Biosensors and Bioelectronics · 2025 · 117339
Open a family to keep every result attached to its sample, method and conditions.
Paraphrased for this database from the authors’ stated interpretations — never quoted verbatim — and kept separate from reported measurements.
The Ab2-MdP magnetic immunosensor detects PCT over 0.05-100 ng mL-1 with an LOD of 0.011 ng mL-1 and agrees with hospital measurements in 15 clinical serum samples.
Caveat: Clinical sample and Bland-Altman table values are available in the rendered SI; no raw time-series data were supplied.
8 · 3.5 Immunosensor performance · Fig. 5; Tables S4-S7 · Linked to 7 structured results
The catalytic reaction rate of MdP is noted by the authors to be lower than that of natural enzymes and requires further investigation.
Caveat: No quantitative comparison to natural enzymes is given in the main text.
8 · 4 Conclusions
MdP contains mixed-valence manganese oxides, specifically Mn3O4 and Mn2O3, with Mn2+, Mn3+ and Mn4+ species detected by XPS.
Caveat: Bulk phase assignment is by PXRD plus HRTEM; surface valence is XPS-derived.
5 · 3.2 Characterization · Fig. 2 · Linked to 7 structured results
The large specific surface area and mesopores of dPCN-224 favour Mn oxide loading and help MdP contact TMB substrate molecules, contributing to higher oxidase-like catalytic efficiency.
Caveat: The causal link is argued from porosity and kinetics rather than isolated surface-area-controlled experiments in the main text.
6 · 3.2; 3.3 Catalytic performance · Fig. S4; Fig. 4E-F · Linked to 4 structured results
Among the Mn-loading variant series, MdP5 (20 mg additive MnCl2, 40.58 ug mg-1 Mn) showed the best TMB catalytic kinetic performance, with Vmax 3.43 x 10-8 M s-1 and Km 0.20 mM.
Caveat: The authors identify MdP5 as best from Michaelis-Menten/Lineweaver-Burk analysis; variant synthetic details are by analogy to the base MdP route except for MnCl2 amount.
6 · 3.3 Catalytic performance · Fig. S9; Table S3 · Linked to 4 structured results
Dodecanoic acid competes with TCPP for Zr cluster sites and introduces mesoporous defects into PCN-224, producing dPCN-224 with 1.9 nm micropores and 4.0 nm mesopores.
Caveat: Pore-size and BET values are reported in the main text and supported by rendered SI Fig. S4.
3 and 6 · 3.1; 3.2 Characterization · Scheme 1; Fig. S4 · Linked to 3 structured results
Introducing mixed-valence manganese oxides enhances the electrical conductivity/electron transfer of dPCN-224, as indicated by higher LSV current, lower EIS impedance and TMB-triggered chronoamperometric current.
Caveat: No direct electronic conductivity value (S cm-1) is reported; evidence is electrochemical proxy behaviour and author interpretation. Electrode/electrolyte details are available in SI section 1.6.
7 · 3.4 Catalytic mechanism · Fig. 4J-L · Linked to 5 structured results
The primary reactive oxygen species in MdP-catalysed TMB oxidation are singlet oxygen and superoxide/peroxyl radicals, while hydroxyl radical is not the main active species.
Caveat: Scavenger controls are reported in main text and Fig. S12; exact inhibition percentages beyond "over 50%" were not tabulated.
7 · 3.4 Catalytic mechanism · Fig. 4G-I; Fig. S12 · Linked to 3 structured results
Names and aliases are kept exactly within the paper’s own identity model.
| Material | Composition | Structure context | Source |
|---|---|---|---|
| Ab1-MBs | PCT capture antibody-conjugated magnetic balls; exact magnetic bead composition not statednot a MOF; magnetic bead support composition not specified · carboxylated bead surface functionalised with PCT capture antibody Ab1 | unknown · CompositeNon-MOF magnetic capture component of the sandwich immunosensor. | SI text · 1.2 Preparation procedures of Ab1-MBs and Ab2-MdP |
| Ab2-MdP | PCT detection antibody-conjugated MnxOy@dPCN-224Zr cluster in dPCN-224; Mn oxide phase · TCPP in dPCN-224; antibody bioconjugate on surface | 3D · CompositeAntibody-functionalised MdP composite used as signal-readout component in the immunosensor. | 3 · 3.1 Development of nanozyme based on MnxOy · Scheme 1 |
| defective PCN-224 (dPCN-224) | defective Zr-TCPP MOF; exact empirical formula not statedZr cluster · TCPP (tetrakis(4-carboxyphenyl)porphyrin) | 3D · PristineDefective PCN-224 with mesoporous defects introduced by dodecanoic acid modulation. | 3 · 3.1 Development of nanozyme based on MnxOy · Scheme 1 |
| MnxOy@dPCN-224 (MdP) | mixed-valence Mn oxides (Mn2O3/Mn3O4) loaded on dPCN-224Zr cluster in dPCN-224; Mn oxide phase containing Mn2+, Mn3+ and Mn4+ · TCPP in dPCN-224 | 3D · CompositeComposite of mixed-valence manganese oxides deposited on/within defective PCN-224. | 1 · Abstract |
| PCN-224 | Zr-TCPP MOF; exact empirical formula not statedZr cluster · TCPP (tetrakis(4-carboxyphenyl)porphyrin) | 3D · PristinePorphyrinic Zr MOF PCN-224; normal PCN-224 used as non-defective control. | 2 · 2.2 Preparation of PCN-224, dPCN-224 and MdP |
Sample form, processing state and composition status define the context for measurements.
| Sample | Form and role | Processing and geometry | Source |
|---|---|---|---|
| Ab1-MBs capture beadsresearch_0626__mat__mat_ab1_mbs | Unknown · Composite Component · Composite | Carboxyl-activated MBs conjugated with PCT capture antibody Ab1, BSA-blocked and stored in 0.10% BSA at 4 C.magnetic balls (MBs) | SI text · 1.2 Preparation procedures of Ab1-MBs and Ab2-MdP |
| Ab2-MdP particlesresearch_0626__mat__mat_ab2_mdp | Powder · Composite Sample · Composite | MdP activated with EDC/NHS in PBS, conjugated with PCT detection antibody Ab2, blocked with BSA and stored in 0.10% BSA at 4 C. | 2 · 2.3 Preparation of Ab1-MBs and Ab2-MdP |
| dPCN-224 nanoparticlesresearch_0626__mat__mat_dpcn224 | Powder · Pristine Control · Pristine Framework | Fuchsia particles after solvothermal DA modulation, HCl/DMF activation, vacuum drying. | 2 · 2.2 Preparation of PCN-224, dPCN-224 and MdP |
| MdP nanoparticlesresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | Dark green MnxOy-loaded dPCN-224 nanoparticles after hydrothermal MnCl2/NH4Cl/NH3 treatment and vacuum drying. | 2 · 2.2 Preparation of PCN-224, dPCN-224 and MdP |
| MdP1 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 4 mg additive MnCl2; Mn element content 7.95 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdP2 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 8 mg additive MnCl2; Mn element content 18.56 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdP3 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 12 mg additive MnCl2; Mn element content 25.43 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdP4 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 16 mg additive MnCl2; Mn element content 32.27 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdP5 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 20 mg additive MnCl2; Mn element content 40.58 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdP6 Mn-loading variantresearch_0626__mat__mat_mdp | Powder · Target Sample · Composite | MdP variant prepared by loading dPCN-224 with 24 mg additive MnCl2; Mn element content 40.75 ug mg-1 by ICP-AES. | 14 · Figures and Tables · Table S2; Table S3 |
| MdPM magnetic immunosensor assay complexresearch_0626__mat__mat_ab2_mdp | Unknown · Composite Sample · Composite | Ab1-MBs/PCT/Ab2-MdP sandwich complex reacted with TMB for PCT detection. | 3 · 2.4 Application of Ab2-MdP in magnetic immunosensor for PCT detection |
| PCN-224 particlesresearch_0626__mat__mat_pcn224 | Powder · Pristine Control · Pristine Framework | Solvothermal ZrCl4/TCPP/benzoic acid product; workup follows dPCN-224 procedure. | 2 · 2.2 Preparation of PCN-224, dPCN-224 and MdP |