Sensing Application — Highly sensitive electrochemiluminescence glucose sensor under alkaline conditions based on glucose oxidase@conductive metal-organic framework nanocapsules

Measurement evidence

Sensing Application

Highly sensitive electrochemiluminescence glucose sensor under alkaline conditions based on glucose oxidase@conductive metal-organic framework nanocapsules · Mao M., Li L., Huang C. et al. · Sensors and Actuators B: Chemical · 2025 · 137595

8 measurement groups · 36 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

OPD colorimetric assay; absorbance at 492 nm

GOx@Zn-HHTP nanocapsule powder · Powder

2 mg composite or free enzyme in pH 7.0-11.0 PBS; HRP, OPD and glucose added; 30 min reaction at 37 degC.

Temperature: 310
Context: GOx-loaded composite vs free GOx control
Measurement source: p002 · 2.4 Biocatalytic activity assay

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
free GOx retained activity at pH 10.0	about 30 %	0.3 fraction	about	Text Approximate	p003 · 3.2 Alkaline stability · Fig. 2B
GOx@Zn-HHTP retained activity at pH 10.0Marked as a best value within this paper	approximately 80 %	0.8 fraction	approximately	Text Approximate	p003 · 3.2 Alkaline stability · Fig. 2B
free GOx activity decrease above pH 8.5	decreased by approximately 60 %	0.6 fraction	approximately	Text Approximate	p003 · 3.2 Alkaline stability · Fig. 2B

ECL glucose calibration at pH 10.0

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

1 mg/mL GOx@Zn-HHTP, pH 10.0 PBS, luminol, glucose; 10 min reaction at 37 degC; ECL assay.

Temperature: 310
Geometry: 0-1 V scan, 100 mV/s, photomultiplier tube -800 V
Context: GOx-loaded composite sensing dispersion
Measurement source: p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
LOD reduction versus pH 7.0Marked as a best value within this paper	25-fold reduction	—	—	Calculated From Reported Exact Reported	p001 · Abstract
pH 10.0 linear range lower boundMarked as a best value within this paper	0.1 uM	1e-7 M	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 10.0 linear range upper boundMarked as a best value within this paper	10 mM	0.01 M	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 10.0 calibration intercept	22326.11 a.u.	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 10.0 limit of detectionMarked as a best value within this paper	0.002 uM (S/N = 3)	2e-9 M	S/N = 3	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor
pH 10.0 calibration R2	R2 = 0.995	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 10.0 calibration slopeMarked as a best value within this paper	3916.84 a.u. per lgCglucose (mM)	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
sensitivity enhancement versus pH 7.0Marked as a best value within this paper	8.5 times	—	—	Calculated From Reported Rounded Reported	p006 · 3.5 Performance of the proposed ECL sensor

ECL glucose calibration at pH 7.0

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

Same sensor and assay conditions as pH 10.0 calibration except pH 7.0 electrolyte.

Temperature: 310
Geometry: 0-1 V scan, 100 mV/s, photomultiplier tube -800 V
Context: neutral-pH comparison for GOx-loaded composite sensor
Measurement source: p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
pH 7.0 calibration intercept	2293.95 a.u.	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 7.0 limit of detection	0.05 uM (S/N = 3)	5e-8 M	S/N = 3	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor
pH 7.0 calibration R2	R2 = 0.997	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D
pH 7.0 calibration slope	459.56 a.u. per lgCglucose (mM)	—	—	Text Exact Reported	p006 · 3.5 Performance of the proposed ECL sensor · Fig. 4D

ECL catalyst concentration optimisation

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

pH 10.0 electrolyte, 10 uM H2O2 and 10 uM glucose; varied GOx@Zn-HHTP concentration.

Context: GOx-loaded composite sensing dispersion
Measurement source: p005 · Fig. 3 caption · Fig. 3A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
optimal GOx@Zn-HHTP concentrationMarked as a best value within this paper	1 mg/mL	1 g L-1	—	Text Exact Reported	p005 · 3.4 Optimization of reaction conditions · Fig. 3A

ECL signal after harsh-environment treatment

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

Free GOx and GOx@Zn-HHTP treated for 30 min at 60 degC, 80 degC, 20x PBS or continuous stirring, then used for detecting 1 mM glucose.

Context: GOx-loaded composite vs free GOx control
Measurement source: p002 · 2.7 Stability and selectivity experiment

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
free GOx signal retention after high-temperature treatment	over 60 %	0.6 fraction	over	Text Approximate	p006 · 3.6 Stability and selectivity · Fig. 5A
GOx@Zn-HHTP ECL retention after harsh treatmentsMarked as a best value within this paper	around 90 % of the control group	0.9 fraction	around	Text Approximate	p006 · 3.6 Stability and selectivity · Fig. 5A

Reuse/reproducibility ECL cycling

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

GOx@Zn-HHTP tested at three glucose concentrations; electrolyte recovered and composite separated by centrifugation between cycles; repeated five times.

Context: GOx-loaded composite sensing dispersion
Measurement source: p006 · 3.6 Stability and selectivity · Fig. 5B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
maximum RSD over five reuse tests	did not exceed 5.68 %	0.0568 fraction	n = 5	Text Exact Reported	p006 · 3.6 Stability and selectivity · Fig. 5B

ECL selectivity against sweat interferents

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

Interferents at 10 mM; glucose at 1 mM; interferents included NaCl, uric acid, lactate, dopamine, fructose, lactose and sucrose.

Context: GOx-loaded composite sensing dispersion
Measurement source: p006 · 3.6 Stability and selectivity · Fig. 5C

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
target glucose concentration in selectivity test	1 mM	0.001 M	—	Text Exact Reported	p006 · 3.6 Stability and selectivity · Fig. 5C
interferent challenge concentration	10 mM	0.01 M	—	Text Exact Reported	p006 · 3.6 Stability and selectivity · Fig. 5C
selectivity response summary	almost no response to 10 mM interferents; strong stable signal for 1 mM glucose and mixed samples	—	—	Text Qualitative	p006-p007 · 3.6 Stability and selectivity · Fig. 5C

Human sweat glucose ECL sensing and standard recovery

GOx@Zn-HHTP ECL glucose sensing dispersion · Unknown

Sweat from three volunteers collected using wearable sweat inducer, diluted tenfold, measured directly or spiked with glucose.

Context: GOx-loaded composite sensor applied to biological samples
Measurement source: p008 · Table S1 · Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
sweat glucose concentration upper bound	0.1 mM	0.0001 M	range	Text Range	p007 · 3.7 Application in sweat glucose sensing · Fig. 5D; Table S1
sweat glucose concentration lower bound	0.05 mM	0.00005 M	range	Text Range	p007 · 3.7 Application in sweat glucose sensing · Fig. 5D; Table S1
standard recovery upper bound	100.56 %	1.0056 fraction	range	Text Range	p007 · 3.7 Application in sweat glucose sensing · Fig. 5D; Table S1
standard recovery lower bound	91.70 %	0.917 fraction	range	Text Range	p007 · 3.7 Application in sweat glucose sensing · Fig. 5D; Table S1
sweat sample #1 detected glucose	0.094 +/- 0.0018 mM	0.000094 M	+/- 0.0018 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #1 found glucose after spike	0.187 +/- 0.0056 mM	0.000187 M	+/- 0.0056 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #1 recovery	96.15 %	0.9615 fraction	n = 3	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #2 detected glucose	0.058 +/- 0.0015 mM	0.000058 M	+/- 0.0015 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #2 found glucose after spike	0.159 +/- 0.0062 mM	0.000159 M	+/- 0.0062 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #2 recoveryMarked as a best value within this paper	100.56 %	1.0056 fraction	n = 3	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #3 detected glucose	0.077 +/- 0.0040 mM	0.000077 M	+/- 0.0040 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #3 found glucose after spike	0.162 +/- 0.0042 mM	0.000162 M	+/- 0.0042 mM	SI Table Exact Reported	p008 · Table S1 · Table S1
sweat sample #3 recovery	91.70 %	0.917 fraction	n = 3	SI Table Exact Reported	p008 · Table S1 · Table S1
glucose spike level for recovery tests	0.10 mM	0.0001 M	—	SI Table Exact Reported	p008 · Table S1 · Table S1