Complete recipeSource: SI
Route 1: Other
p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP
Metal precursors2 mL Zn(CH3COO)2 solution (206 mM)
Linker precursors2 mL aqueous 2.72 M 2-methylimidazole (2-MeIm)
Solventswater
Additives2 mg PVP; 0.5 mg GOx; 0.5 mg L-cysteine (Cys)
Atmospherenot reported; room-temperature ambient handling implied
Temperatureroom temperature; vacuum dried at 37 degC
Time0.033 h initial stir + 0.050 h after Cys addition + 1.5 h ageing + 12 h vacuum drying
Work-upcentrifugation at 6000 rpm for 5 min; washed three times with water
Activationvacuum-dried at 37 degC for 12 h and stored in powder form
Scalability contextMilligram-scale batch; no scale-up discussion.
Show 6 structured reagent records
| Role | Reagent | Amount / concentration | Source |
|---|---|---|---|
| Linker | 2-methylimidazole (2-MeIm) | 2 mL aqueous solution · 2.72 M | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |
| Additive | polyvinylpyrrolidone (PVP) | 2 mg | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |
| Other | glucose oxidase (GOx) | 0.5 mg | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |
| Additive | L-cysteine (Cys) | 0.5 mg | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |
| Metal Source | Zn(CH3COO)2 | 2 mL solution · 206 mM | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |
| Solvent | water | Not specified | p004 · Experimental Procedure: Procedures for synthesis of GOx@Zn-HHTP |