Synthesis · Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel

Complete recipeSource: Main

Route 1: Other

3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel

Metal precursorsCu3(HHTP)2 dispersion, 2 uL, 1 mg/mL

Linker precursorsSA, SB and T-2 aptamer DNA strands, each 300 uM, mixed 1:1:1

Solventsaqueous/PBS media

Additivessodium hyaluronate (HA), EDC, NHS, PEI

Atmospherenot reported

Temperature37

Time3.25 total staged incubation (2 h DNA mixture; 15 min HA activation; 1 h hydrogel reaction)

Work-upwashed twice with 1x PBS solution and stored at 4 C

ActivationHA activated by sequential addition of Cu3(HHTP)2, EDC and NHS at 37 C for 15 min

Show 8 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	SA	mixed in 1:1:1 SA/SB/Apt ratio · 300 uM	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Other	SB	mixed in 1:1:1 SA/SB/Apt ratio · 300 uM	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Other	T-2 aptamer	mixed in 1:1:1 SA/SB/Apt ratio · 300 uM	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Other	Cu3(HHTP)2	2 uL · 1 mg/mL	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Additive	EDC solution	2 uL · 97.6 mg/mL	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Additive	NHS solution	2 uL · 23 mg/mL	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Additive	HA solution	4 uL · 10 mg/mL	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel
Additive	PEI	mixed with SA-SB-Apt at 3.3:2.7 volume ratio; 6 uL mixture used · 2 mg/mL	3 · 2.3. Preparation of T-2 toxin-responsive DNA hydrogel

Complete recipeSource: Main

3 · 2.2. Preparation of Cu3(HHTP)2

Metal precursorsCuSO4.5H2O, 410 mg in 9 mL ultrapure water

Linker precursorsHHTP, 233 mg in DMF (3 mL, reported 0.718 mM) plus 24 mL ultrapure water

SolventsDMF; ultrapure water; wash solvents water, ethanol and acetone

Atmospherenot reported

Temperature80

Time12

Work-upcooled to room temperature; washed twice with water, ethanol and acetone; dried

Activationglassware and magnetic stir bars soaked in aqua regia for at least 12 h, rinsed and dried before synthesis

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	2,3,6,7,10,11-hexahydroxytriphenylene hydrate (HHTP)	233 mg · reported 0.718 mM in 3 mL DMF before water addition	3 · 2.2. Preparation of Cu3(HHTP)2
Metal Source	copper sulfate pentahydrate (CuSO4.5H2O)	410 mg	3 · 2.2. Preparation of Cu3(HHTP)2
Solvent	dimethylformamide (DMF)	3 mL	3 · 2.2. Preparation of Cu3(HHTP)2
Solvent	ultrapure water	24 mL added to HHTP solution; 9 mL for CuSO4.5H2O solution	3 · 2.2. Preparation of Cu3(HHTP)2
Acid	aqua regia	glassware soak, at least 12 h	3 · 2.2. Preparation of Cu3(HHTP)2