Sensing Application — Advanced dual-signal point-of-care testing platform for sensitive T-2 toxin detection: Integrating copper-based conductive MOF with target-responsive DNA hydrogel

8 measurement groups · 46 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

UV-vis colourimetric feasibility and temperature response

UV-visible and optical absorption spectroscopy

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

TMB/H2O2 assay with DNA hydrogel with and without T-2 toxin; absorbance peak at 652 nm and temperature change under laser irradiation

Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 5 · 3.4. Feasibility analysis · Fig. 3A-B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Delta-T without T-2 toxin	Delta-T = 11.1 C	—	—	Visual Estimate Rounded Reported	5 · 3.4. Feasibility analysis · Fig. 3A
TMB+ characteristic absorption peak	approximately 652 nm	—	—	Text Approximate	3 · 3.3. Principle for T-2 toxin · Scheme 1
Delta-T with T-2 toxinMarked as a best value within this paper	Delta-T = 37.8 C	—	—	Visual Estimate Rounded Reported	5 · 3.4. Feasibility analysis · Fig. 3A

Optimisation of hydrogel and catalytic reaction conditions

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

Optimised Cu3(HHTP)2 concentration in hydrogel, hydrolysis temperature, TMB concentration and H2O2 concentration

Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 6 · 3.5. Optimization of experimental conditions · Fig. 4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Selected Cu3(HHTP)2 concentration in DNA hydrogelMarked as a best value within this paper	1 mg/mL	—	—	Text Exact Reported	5 · 3.5. Optimization of experimental conditions · Fig. 4A
Selected H2O2 concentrationMarked as a best value within this paper	500 mM	—	—	Text Exact Reported	6 · 3.5. Optimization of experimental conditions · Fig. 4D
Selected hydrolysis temperatureMarked as a best value within this paper	57 C	—	—	Text Exact Reported	5 · 3.5. Optimization of experimental conditions · Fig. 4B
Selected TMB concentrationMarked as a best value within this paper	30 mM	—	—	Text Exact Reported	6 · 3.5. Optimization of experimental conditions · Fig. 4C

Steady-state peroxidase-like kinetic experiments with H2O2 and TMB

Cu3(HHTP)2 solution/dispersion for photothermal and catalytic tests · Unknown

Initial velocities measured under varying concentrations of H2O2 and TMB; Lineweaver-Burk plots in Figure S1

Context: pristine Cu3(HHTP)2 catalytic component
Measurement source: 3 · 3.2. Peroxidase-like activity · Figure S1; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Michaelis-Menten constant for H2O2	0.22 mM	—	—	Text Exact Reported	3 · 3.2. Peroxidase-like activity · Figure S1
Michaelis-Menten constant for TMB	0.24 mM	—	—	Text Exact Reported	3 · 3.2. Peroxidase-like activity · Figure S1
SI Table S1 Michaelis-Menten constant for H2O2	0.17 mM	—	—	SI Table Exact Reported	4 · Supporting Information · Table S1
SI Table S1 Michaelis-Menten constant for TMB	0.40 mM	—	—	SI Table Exact Reported	3 · Supporting Information · Table S1
SI Table S1 maximum reaction velocity for H2O2	7.7 x 10^-7 M/s	7.7e-7 M/s	—	SI Table Exact Reported	4 · Supporting Information · Table S1
SI Table S1 maximum reaction velocity for TMB	5.2 x 10^-7 M/s	5.2e-7 M/s	—	SI Table Exact Reported	3 · Supporting Information · Table S1
Maximum reaction velocity for H2O2	2.9 x 10^-7 M/s	—	—	Text Exact Reported	3 · 3.2. Peroxidase-like activity · Figure S1
Maximum reaction velocity for TMBMarked as a best value within this paper	5.3 x 10^-7 M/s	—	—	Text Exact Reported	3 · 3.2. Peroxidase-like activity · Figure S1

Standard-addition recovery tests in real samples

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

Moldy corn extract and barley tea spiked with T-2 toxin; n = 3

Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 8 · 3.7. Analysis of actual samples · Table 2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Barley tea detected T-2 toxin, 100 ng/mL added	98.99 ng/mL	—	RSD 2.03%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Barley tea recovery, 100 ng/mL added	97.48%	—	RSD 2.03%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Barley tea detected T-2 toxin, 40 ng/mL added	39.70 ng/mL	—	RSD 5.98%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Barley tea recovery, 40 ng/mL added	98.03%	—	RSD 5.98%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Barley tea detected T-2 toxin, 60 ng/mL added	60.80 ng/mL	—	RSD 3.82%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Barley tea recovery, 60 ng/mL added	100.02%	—	RSD 3.82%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn detected T-2 toxin, 0 ng/mL added	40.74 ng/mL	—	RSD 4.01%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn recovery, 0 ng/mL added	99.63%	—	RSD 4.01%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn detected T-2 toxin, 20 ng/mL added	61.06 ng/mL	—	RSD 5.53%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn recovery, 20 ng/mL addedMarked as a best value within this paper	100.05%	—	RSD 5.53%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn detected T-2 toxin, 60 ng/mL added	99.64 ng/mL	—	RSD 3.66%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2
Moldy corn recovery, 60 ng/mL added	98.56%	—	RSD 3.66%	Table Exact Reported	8 · 3.7. Analysis of actual samples · Table 2

Selectivity, reproducibility and storage stability tests

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

AFB1, OTA and ZEN interference tests; triplicate reproducibility at 20, 40 and 80 ng/mL; hydrogel storage at 4 C for 0-30 d

Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 7 · 3.6. Performance analysis of the POCT method · Fig. 6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Reproducibility test target levels	20, 40, 80 ng/mL	—	n = 3	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 6B
Interfering toxin level in selectivity test	1000 ng/mL	—	—	Caption Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 6A caption
T-2 toxin level in selectivity test	100 ng/mL	—	—	Caption Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 6A caption
Hydrogel storage-stability duration	0-30 days at 4 C	—	—	Text Range	7 · 3.6. Performance analysis of the POCT method · Fig. 6C
Hydrogel storage stability	good stability throughout the testing period	—	—	Text Qualitative	7 · 3.6. Performance analysis of the POCT method · Fig. 6C

Catalytic reaction pH optimisation

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

Figure S2 compares delta-T and absorbance responses from pH 3 to pH 8 in the catalytic sensing reaction.

Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 4 · Supporting Information · Figure S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Approximate absorbance at pH 7	about 0.37 a.u. at pH 7	—	—	Figure Axis Approximate	4 · Supporting Information · Figure S2
Approximate delta-T at pH 7	about 28 C at pH 7	—	—	Figure Axis Approximate	4 · Supporting Information · Figure S2
High-response pH region	pH 5-8 maintained high absorbance/delta-T response	—	—	Visual Estimate Range	4 · Supporting Information · Figure S2

SI comparison of photothermal materials in POCT applications

Cu3(HHTP)2 solution/dispersion for photothermal and catalytic tests · Unknown

Table S2 compares photothermal conversion efficiency, signal readout and LOD for PDA and Cu3(HHTP)2 entries.

Context: Cu3(HHTP)2 photothermal component used in the POCT method
Measurement source: 4 · Supporting Information · Table S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
SI Table S2 Cu3(HHTP)2 colourimetric LOD	12.83 ng/mL	—	—	SI Table Exact Reported	4 · Supporting Information · Table S2
SI Table S2 Cu3(HHTP)2 photothermal conversion efficiencyMarked as a best value within this paper	58.93%	—	—	SI Table Exact Reported	4 · Supporting Information · Table S2

Dual-signal T-2 toxin calibration

POCT reaction solution after hydrogel response and TMB/H2O2 addition · Unknown

T-2 toxin concentrations 0, 5, 10, 20, 40, 60, 80, 100, 150 and 200 ng/mL; colour, 652 nm absorbance and delta-T recorded

Geometry: microplate wells; camera and microplate reader; thermometer after 808 nm irradiation
Context: Cu3(HHTP)2-loaded DNA hydrogel sensing system
Measurement source: 6 · 3.6. Performance analysis of the POCT method · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Absorbance calibration intercept at 652 nm	0.1723	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5C
Absorbance calibration R2	R2 = 0.994	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5C
Absorbance calibration slope at 652 nm	Y = 0.00286 C + 0.1723	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5C
Limit of detectionMarked as a best value within this paper	1.67 ng/mL	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Table 1
Linear/detection rangeMarked as a best value within this paper	5-200 ng/mL	—	—	Text Range	7 · 3.6. Performance analysis of the POCT method · Fig. 5C-D
Total procedure time	within 25 min	—	—	Text Approximate	3 · 2.4. Detection of T-2 toxin
Temperature calibration intercept	11.6567 C	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5D
Temperature calibration R2Marked as a best value within this paper	R2 = 0.998	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5D
Temperature calibration slope	DeltaT = 0.2479 C + 11.6567	—	—	Text Exact Reported	7 · 3.6. Performance analysis of the POCT method · Fig. 5D