Sensing Application — NiPd mediated by conductive metal organic frameworks with facilitated electron transfer for assaying of H2O2 released from living cells

4 measurement groups · 12 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

NiPd@Ni3HHTP2/GC · Electrode

N2-saturated 10 mM PBS (pH 7.4) at -0.20 V vs Ag/AgCl with successive H2O2 additions from 1 uM to 45 mM.

Atmosphere: N2-saturated electrolyte
Geometry: NiPd@Ni3HHTP2/GC working electrode
Context: target sensor electrode
Measurement source: 5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4A-B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
H2O2 calibration R2	R2 = 0.996	—	—	Figure Axis Rounded Reported	6 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4B
H2O2 calibration slope	y = -4.55x - 2.06	—	—	Figure Axis Rounded Reported	6 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4B
H2O2 detection limitMarked as a best value within this paper	0.8 uM (3sigma/k, n = 5)	0.0008 mM	n = 5	Text Exact Reported	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4A-B
H2O2 linear range upper boundMarked as a best value within this paper	45 mM	45 mM	—	Text Exact Reported	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4A-B
H2O2 linear range lower bound	1 uM	0.001 mM	—	Text Exact Reported	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4A-B
H2O2 amperometric response time	less than 10 s	—	—	Text Approximate	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4A-B

NiPd@Ni3HHTP2/GC · Electrode

2 mL 10 mM PBS (pH 7.4) containing HeLa cells; PMA stimulation; -0.20 V vs Ag/AgCl; catalase control.

Geometry: NiPd@Ni3HHTP2/GC working electrode
Context: target sensor electrode in biological application
Measurement source: 6 · 3.4. Determination of H2O2 released from live cells · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Cell-detection controls	No apparent current for only PMA or HeLa cells; catalase addition decreased cathodic current	—	—	Text Qualitative	6 · 3.4. Determination of H2O2 released from live cells · Fig. 5C
H2O2 released from HeLa cells at 5.0 uM PMAMarked as a best value within this paper	3.22 uM	0.00322 mM	—	Text Exact Reported	6 · 3.4. Determination of H2O2 released from live cells · Fig. 5C
H2O2 released from HeLa cells at lower PMA stimulation	1.16 uM	0.00116 mM	—	Text Exact Reported	6 · 3.4. Determination of H2O2 released from live cells · Fig. 5C

NiPd@Ni3HHTP2/GC · Electrode

Five electrodes in N2-saturated 10 mM PBS (pH 7.4) containing 1 mM H2O2; ten repeated amperometric measurements.

Atmosphere: N2-saturated electrolyte
Geometry: NiPd@Ni3HHTP2/GC working electrodes
Context: target sensor electrode
Measurement source: 6 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4E-F

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Five-electrode current variation	less than 5.6%	—	—	Text Approximate	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4E
Current decrease after 10 repeated amperometric measurementsMarked as a best value within this paper	decreased by 2.1%	—	—	Text Exact Reported	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4F

NiPd@Ni3HHTP2/GC · Electrode

N2-saturated 10 mM PBS (pH 7.4) at -0.40 V vs Ag/AgCl with additions of H2O2, AA, UA, DA, 5-HT, Gly, Glu and Cys.

Atmosphere: N2-saturated electrolyte
Geometry: NiPd@Ni3HHTP2/GC working electrode
Context: target sensor electrode
Measurement source: 6 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4C-D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Interference from biological species and amino acids	interferences smaller than 6.2%	—	—	Text Exact Reported	5 · 3.3. Analytical performance of NiPd@Ni3HTTP2/GC towards H2O2 · Fig. 4C-D