Synthesis · NiPd mediated by conductive metal organic frameworks with facilitated electron transfer for assaying of H2O2 released from living cells

7 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

Route 1: Drop Cast

2 · 2.4. Preparation of different modified electrodes

Metal precursorsNi3HHTP2

Solventsnot specified for drop-cast dispersion

Atmospheredried under nitrogen before drop-casting

Temperatureroom temperature drying

Substrate orientationglassy carbon electrode, 3.0 mm diameter

Work-upPrepared by the same GC cleaning/drop-casting method as NiPd@Ni3HHTP2/GC.

Scalability contextElectrode-level drop-casting; mass loading 0.14 mg cm-2.

Show 1 structured reagent record

Role	Reagent	Amount / concentration	Source
Other	Ni3HHTP2	mass loading 0.14 mg cm-2	2 · 2.4. Preparation of different modified electrodes

Partial recipeSource: Main

Route 2: Hydrothermal

2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Metal precursors456 mg nickel acetate tetrahydrate

Linker precursors200 mg HHTP

Solvents28 mL deionized water

Atmospherenot reported

Temperaturenot reported for reaction; dried at 49 C

Time24 h reaction; 24 h drying

Work-upSonicated 10 min; stirred in oil bath 24 h; suction filtered after cooling; washed with deionized water and acetone several times.

ActivationVacuum oven drying for 24 h at 49 C and 50 kPa.

Scalability context100 mL round-bottom flask batch synthesis.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	HHTP	200 mg	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Metal Source	nickel acetate tetrahydrate	456 mg	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Solvent	deionized water	28 mL	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Partial recipeSource: Main

Route 3: Drop Cast

2 · 2.4. Preparation of different modified electrodes

Metal precursorsNiPd nanoparticles

Solventsnot specified for drop-cast dispersion

Atmospheredried under nitrogen before drop-casting

Temperatureroom temperature drying

Substrate orientationglassy carbon electrode, 3.0 mm diameter

Work-upPrepared by the same GC cleaning/drop-casting method as NiPd@Ni3HHTP2/GC.

Scalability contextElectrode-level drop-casting; mass loading 25 ug cm-2.

Show 1 structured reagent record

Role	Reagent	Amount / concentration	Source
Other	NiPd nanoparticles	mass loading 25 ug cm-2	2 · 2.4. Preparation of different modified electrodes

Complete recipeSource: Main

Route 4: Drop Cast

2 · 2.4. Preparation of different modified electrodes

Metal precursorsNiPd@Ni3HHTP2 hybrid

Solventsdeionized water; ethanol/water 1:1 cleaning solution

Atmospheredried under nitrogen before drop-casting

Temperatureroom temperature drying

Substrate orientationglassy carbon electrode, 3.0 mm diameter

Work-upGC polished with 1.0 and 0.3 um alumina slurry; rinsed; sonicated in water and ethanol/water 1:1 for 5 min each; dried under nitrogen; 5 uL hybrid drop-cast and dried.

Scalability contextElectrode-level drop-casting; mass loading 0.165 mg cm-2.

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	NiPd@Ni3HHTP2 hybrid	5 uL	2 · 2.4. Preparation of different modified electrodes
Other	glassy carbon electrode	3.0 mm diameter	2 · 2.4. Preparation of different modified electrodes

Complete recipeSource: Main

Route 5: Other

2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Metal precursorsNiPd NPs solution; Ni3HHTP2 water solution

Linker precursorsHHTP contained in preformed Ni3HHTP2

Solventswater

Atmospherenot reported

Temperature4

Time2

Work-upMixed Ni3HHTP2 water solution (2 mg mL-1) and NiPd NPs solution at 10:1 volume ratio; sonicated for 2 h at 4 C.

Scalability contextSimple solution mixing/sonication route.

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	Ni3HHTP2 water solution	10 volume equivalents · 2 mg mL-1	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Other	NiPd NPs solution	1 volume equivalent	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Partial recipeSource: Main

Route 6: Other

2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Metal precursors13.0 mg NiCl2; 5 mL H2PdCl4 solution containing 7.5 mg H2PdCl4

Solvents100 mL deionized water plus final 5 mL water dispersion

Additives51.6 mg trisodium citrate

AtmosphereN2 purged for 30 min to remove dissolved oxygen

Temperaturenot reported

Time0.333 h after NaBH4 addition and H2PdCl4 injection, plus N2 purge

Oxidant / reductant10 mL freshly prepared NaBH4 solution (2 mg mL-1)

Work-upWashed with water several times and centrifuged at 8000 rpm for 10 min each time.

Scalability context250 mL three-necked flask batch synthesis.

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Additive	trisodium citrate	51.6 mg	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Metal Source	NiCl2	13.0 mg	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Solvent	deionized water	100 mL	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Reductant	NaBH4 solution	10 mL · 2 mg mL-1	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid
Metal Source	H2PdCl4	5 mL solution; 7.5 mg H2PdCl4	2 · 2.3. Synthesis of NiPd@Ni3HHTP2 hybrid

Partial recipeSource: Main

Route 7: Drop Cast

2 · 2.4. Preparation of different modified electrodes

Metal precursorsNiPd nanoparticles; commercial ZIF-8

Solventsnot specified for drop-cast dispersion

Atmospheredried under nitrogen before drop-casting

Temperatureroom temperature drying

Substrate orientationglassy carbon electrode, 3.0 mm diameter

Work-upPrepared by the same GC cleaning/drop-casting method as NiPd@Ni3HHTP2/GC.

Scalability contextElectrode-level drop-casting; mass loading 0.165 mg cm-2.

Show 1 structured reagent record

Role	Reagent	Amount / concentration	Source
Other	NiPd@ZIF-8	mass loading 0.165 mg cm-2 · NiPd:ZIF-8 mass ratio 5:28	2 · 2.4. Preparation of different modified electrodes