Sensing Application — Metal-organic framework nanomaterials as novel signal probes for electron transfer mediated ultrasensitive electrochemical immunoassay

5 measurement groups · 23 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Au-MOFs-Ab2-CRP/anti-CRP/Pt-COFs/GCE immunosensor · Electrode

Electrochemical response to a series of CRP target concentrations under optimised conditions.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
calibration equation intercept	9.796 x 10^-7	—	—	Text Exact Reported	6 · Analytical Performance · Figure 6b
calibration equation slopeMarked as a best value within this paper	Delta I_peak = 8.1135 x 10^-6 lg C + 9.796 x 10^-7	—	—	Text Exact Reported	6 · Analytical Performance · Figure 6b
CRP linear dynamic range upper boundMarked as a best value within this paper	400 ng/mL	—	—	Text Exact Reported	6 · Analytical Performance · Figure 6
CRP linear dynamic range lower boundMarked as a best value within this paper	1 ng/mL	—	—	Text Exact Reported	6 · Analytical Performance · Figure 6
CRP detection limitMarked as a best value within this paper	0.2 ng/mL determined by 3delta/slope	—	—	Text Exact Reported	6 · Analytical Performance · Figure 6

Au-MOFs-Ab2-CRP/anti-CRP/Pt-COFs/GCE immunosensor · Electrode

Optimised pH, anti-CRP concentration, anti-CRP incubation time, and CRP incubation time based on immunosensor response.

Geometry: sandwich immunosensor on Pt-COFs/GCE
Context: full application construct
Measurement source: 6 · Optimization of the Experimental Conditions of the Immunoassay · Figure 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
optimal anti-CRP concentrationMarked as a best value within this paper	40 ug/mL	—	—	Text Exact Reported	6 · Optimization of the Experimental Conditions of the Immunoassay · Figure 5b
optimal anti-CRP incubation timeMarked as a best value within this paper	60 min	—	—	Text Exact Reported	6 · Optimization of the Experimental Conditions of the Immunoassay · Figure 5c
optimal CRP incubation timeMarked as a best value within this paper	60 min	—	—	Text Exact Reported	6 · Optimization of the Experimental Conditions of the Immunoassay · Figure 5d
optimal pHMarked as a best value within this paper	pH 4.5	—	—	Text Exact Reported	6 · Optimization of the Experimental Conditions of the Immunoassay · Figure 5a

Au-MOFs-Ab2-CRP/anti-CRP/Pt-COFs/GCE immunosensor · Electrode

Five replicate measurements at 20 ng/mL CRP; 100 successive CV scans; storage in pH 7.4 PBS at 4 C for 1 week; urea soaking for regeneration.

Geometry: sandwich immunosensor on Pt-COFs/GCE
Context: full application construct
Measurement source: 6 · Precision, Reproducibility, Stability, and Reusability of Immunosensor

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
response retained after 100 CV scans	96% of the initial response after 100 CV scans	—	—	Text Exact Reported	6 · Precision, Reproducibility, Stability, and Reusability of Immunosensor
intra-assay variation coefficient	3.8% with the same concentration of CRP (20 ng/mL)	—	—	Text Exact Reported	6 · Precision, Reproducibility, Stability, and Reusability of Immunosensor
storage response retention after one week	90% of the initial value after storage in pH 7.4 PBS at 4 C for 1 week	—	—	Text Exact Reported	6 · Precision, Reproducibility, Stability, and Reusability of Immunosensor

Au-MOFs-Ab2-CRP/anti-CRP/Pt-COFs/GCE immunosensor · Electrode

Response to CRP at 100 ng/mL compared with CEA, HCG, Gly, and Glu interferents at 1 ug/mL.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CEA interferent current	approximately -3.8 uA at 1 ug/mL CEA	—	visual estimate from bar chart	Figure Axis Approximate	6 · Analytical Performance · Figure 7
CRP selectivity current	approximately -15 uA at 100 ng/mL CRP	—	visual estimate from bar chart	Figure Axis Approximate	6 · Analytical Performance · Figure 7
Glu interferent current	approximately -2.3 uA at 1 ug/mL Glu	—	visual estimate from bar chart	Figure Axis Approximate	6 · Analytical Performance · Figure 7
Gly interferent current	approximately -2.4 uA at 1 ug/mL Gly	—	visual estimate from bar chart	Figure Axis Approximate	6 · Analytical Performance · Figure 7
HCG interferent current	approximately -1.8 uA at 1 ug/mL HCG	—	visual estimate from bar chart	Figure Axis Approximate	6 · Analytical Performance · Figure 7

Au-MOFs-Ab2-CRP/anti-CRP/Pt-COFs/GCE immunosensor · Electrode

CRP added to diluted 10% serum samples; recovery experiment at 50.00 ng/mL added CRP.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
serum spike concentration	50.00 ng/mL added	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1
average serum recovery	95.12%	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1
serum found concentration replicate 1	46.65 ng/mL	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1
serum found concentration replicate 2	46.52 ng/mL	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1
serum found concentration replicate 3	49.51 ng/mL	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1
serum recovery RSD	3.56%	—	—	Table Exact Reported	7 · Application in Analysis of Serum Samples · Table 1