Electrochemistry Application — Development of an electrochemiluminescence aptasensor combining covalent-triazine framework emitter with exonuclease III-driven DNA walker for sensitive CEA detection

5 measurement groups · 22 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

EIS binding-affinity assay

Impedance spectroscopy

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

Rct monitored at varying DNA concentrations: CTF with ssDNA, CuxMn3-x(HITP)2 with Hp, and MCH/ssDNA/CTF with Hp/CuxMn3-x(HITP)2 after CEA recognition. SI: EIS in 0.1 M PBS pH 7.4 containing 2.5 mM [Fe(CN)6]3-/4-.

Geometry: CTF and Hp/CuxMn3-x(HITP)2 modified electrodes
Context: DNA binding to CTF, conductive MOF, and composite aptasensor
Measurement source: 7 · 3.3 Feasibility analysis · Figs. S12-S13

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Affinity constant of Hp on CuxMn3-x(HITP)2Marked as a best value within this paper	5.24 uM-1	—	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S12
Affinity constant of rHp/CuxMn3-x(HITP)2 on MCH/ssDNA/CTF	0.03 mL g-1	—	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S12
Affinity constant of ssDNA on CTF	0.25 uM-1	—	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S12
Rct after CEA introductionMarked as a best value within this paper	1.27 kOhm	1270 Ohm	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S13
Rct after dsDNA soaking	983.0 Ohm	—	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S13
Rct of Hp/CuxMn3-x(HITP)2/AE	881.0 Ohm	—	—	Text Exact Reported	7 · 3.3 Feasibility analysis · Fig. S13

Cyclic voltammetry (CV)

Voltammetry

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

CV curves at each fabrication step in 0.1 M PBS containing 5 mM [Fe(CN)6]3-/4- and 0.1 M KCl; SI S1.6 records -0.4 to 0.8 V vs Ag/AgCl at 50 mV s-1.

Geometry: three-electrode system with modified Au working electrode
Context: stepwise fabrication comparison
Measurement source: 6 · Figure 2 caption · Fig. 2b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CV redox peak current after rHp/MOF recognition	higher redox/reduction peak current than MCH/ssDNA/CTF/AE	—	—	Qualitative Qualitative	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2b

Electrochemiluminescence (ECL) profile

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

ECL profiles for bare and modified electrodes in 0.1 M PBS containing 0.1 M K2S2O8 by Fig. 2/S7 captions; SI S1.6 method says potential range -2.2 to -1.2 V in PBS containing 1.0 M K2S2O8, PMT 800 V, scan rate 0.15 V s-1.

Geometry: three-electrode system; modified Au working electrode, Pt counter, Ag/AgCl reference
Context: stepwise fabrication comparison including pristine CTF/AE and final MOF-coupled sensor
Measurement source: 6 · Figure 2 caption · Fig. 2c

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
ECL intensity of bare AE	13 a.u.	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2c
ECL intensity of CTF/AE	15,426 a.u.	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2c
ECL intensity after CEA-triggered CuxMn3-x(HITP)2 couplingMarked as a best value within this paper	17,082 a.u.	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2c
ECL intensity of MCH/ssDNA/CTF/AE	12,785 a.u.	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2c
Photomultiplier tube voltage	800 V	—	—	Text Exact Reported	SI text · S1.6 Electrochemiluminescence (ECL) and electrochemical measurements
ECL scan rate	0.15 V s-1	—	—	Text Exact Reported	SI text · S1.6 Electrochemiluminescence (ECL) and electrochemical measurements
Maximum ECL potential	-1.94 V vs. Ag/AgCl (saturated KCl)	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. S7
ECL intensity of ssDNA/CTF/AE	14,426 a.u.	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2c

Relative ECL quantum efficiency from ECL-potential plots

CuxMn3-x(HITP)2/AE · Electrode

CTF/AE and CuxMn3-x(HITP)2/AE compared with Ru(bpy)3(2+)/AE reference using ECL-potential/current-potential plots in Fig. S10.

Geometry: three-electrode ECL setup
Context: CTF emitter and conductive MOF emitter comparison
Measurement source: 6 · 3.3 Feasibility analysis · Fig. S10

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Relative ECL quantum efficiency of CTFMarked as a best value within this paper	33.4 % of Ru(bpy)3(2+)	0.334 relative to Ru(bpy)3(2+)	—	Text Exact Reported	6 · 3.3 Feasibility analysis · Fig. S10
Relative ECL quantum efficiency of CuxMn3-x(HITP)2	1.1 % of Ru(bpy)3(2+)	0.011 relative to Ru(bpy)3(2+)	—	Text Exact Reported	6 · 3.3 Feasibility analysis · Fig. S10

Electrochemical impedance spectroscopy (EIS)

Impedance spectroscopy

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

Stepwise aptasensor fabrication assessed in PBS (0.1 M, pH 7.4) containing 5.0 mM [Fe(CN)6]3-/4-; Rct fitted to equivalent circuit. SI S1.6: 0.01 Hz to 100 kHz, DC potential 0.18 V, amplitude 5 mV.

Geometry: three-electrode system with modified Au working electrode
Context: bare AE, CTF/AE, ssDNA/CTF/AE, MCH/ssDNA/CTF/AE and final CuxMn3-x(HITP)2-coupled aptasensor compared
Measurement source: 4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Rct of bare AE	40.40 Ohm	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a
Rct of CTF/AE	533.02 Ohm	—	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a
Rct after CuxMn3-x(HITP)2 coupling/detectionMarked as a best value within this paper	1.18 kOhm	1180 Ohm	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a
Rct of MCH/ssDNA/CTF/AE	1.52 kOhm	1520 Ohm	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a
Rct of ssDNA/CTF/AE	1.38 kOhm	1380 Ohm	—	Text Exact Reported	4 · 3.2 Construction of CTF-based ECL aptasensor · Fig. 2a