cMOF Evidence Base
Sensing Application — Development of an electrochemiluminescence aptasensor combining covalent-triazine framework emitter with exonuclease III-driven DNA walker for sensitive CEA detection

Measurement evidence

Sensing Application

Development of an electrochemiluminescence aptasensor combining covalent-triazine framework emitter with exonuclease III-driven DNA walker for sensitive CEA detection · Zhang S., Li Z., Wang Y. et al. · Microchemical Journal · 2025 · 114388

4 measurement groups · 73 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

CEA concentration titration by ECL

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

Optimised CTF-based ECL aptasensor used to detect CEA; calibration covers 1 pg mL-1 to 50 ng mL-1 and LOD calculated as 3SB/m.

Geometry
CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE aptasensor
Context
final conductive-MOF/CTF composite sensor
Measurement source
8 · 3.4 Quantitative analysis of CEA · Fig. 4a-b
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Calibration intercept for Delta I_ECL vs lg C_CEA681.3——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4b
Calibration correlation coefficient0.99——Text
Rounded Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4b
Calibration slope for Delta I_ECL vs lg C_CEA1009.3——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4b
CEA detection range upper bound50 ng mL-150000 pg mL-1—Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4a-b
CEA detection range lower bound1.0 pg mL-1——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4a-b
Limit of detection for CEAMarked as a best value within this paper2.91 fg mL-10.00291 pg mL-1—Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Fig. 4b
Optimised aptamer concentration200 nM——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Figs. S14-S15
Optimised CTF dosage0.8 mg mL-1——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Figs. S14-S15
Optimised dsDNA concentration from SI discussion200 nM——Text
Exact Reported		SI text · S5 Optimization of experimental conditions · Figs. S14-S15
Optimised Hp/CuxMn3-x(HITP)2-cDNA incubation time2 h——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Figs. S14-S15
Optimised rHp/CuxMn3-x(HITP)2 with MCH/ssDNA/CTF incubation time2 h——Text
Exact Reported		8 · 3.4 Quantitative analysis of CEA · Figs. S14-S15

SI comparison table for CEA detection methods

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

Table S1 compares detection method, linear range and LOD of the proposed CTF/CuxMn3-x(HITP)2 ECL sensor against reported CEA methods.

Geometry
CTF/CuxMn3-x(HITP)2 ECL aptasensor on Au electrode
Context
final conductive-MOF/CTF composite sensor; only the this-work row is first-hand evidence
Measurement source
25 · S6 Comparison of methods for CEA detection · Table S1
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Table S1 this-work detection range upper bound5x10^4 pg mL-1——SI Table
Exact Reported		25 · S6 Comparison of methods for CEA detection · Table S1
Table S1 this-work detection range lower bound1 pg mL-1——SI Table
Exact Reported		25 · S6 Comparison of methods for CEA detection · Table S1
Table S1 this-work LODMarked as a best value within this paper0.065 pg mL-1——SI Table
Exact Reported		25 · S6 Comparison of methods for CEA detection · Table S1

Standard addition recovery in human serum with ELISA comparison

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

CEA standards spiked into pretreated human serum and quantified with aptasensor and commercial ELISA kit.

Geometry
CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE aptasensor
Context
final conductive-MOF/CTF composite sensor
Measurement source
26 · S7 Real sample analysis · Table S2
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
F-test critical value used for ELISA comparisonF0.95 = 16——Text
Exact Reported		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
Human serum recovery upper bound107.0 %——Text
Range		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
Human serum recovery lower bound96.2 %——Text
Range		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
Human serum recovery RSD upper bound5.12 %——Text
Range		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
Human serum recovery RSD lower bound3.17 %——Text
Range		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
t-test critical value used for ELISA comparisont0.05 = 2.78——Text
Exact Reported		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Table S2
Table S2 added CEA concentration (0.001 ng mL-1 row)0.001 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (0.001 ng mL-1 added)No detected——SI Table
Qualitative		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (0.001 ng mL-1 added)0.00107 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (0.001 ng mL-1 added)107 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (0.001 ng mL-1 added)3.25 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (0.01 ng mL-1 row)0.01 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (0.01 ng mL-1 added)0.0098 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (0.01 ng mL-1 added)1.83——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (0.01 ng mL-1 added)0.0105 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (0.01 ng mL-1 added)105 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (0.01 ng mL-1 added)4.23 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (0.01 ng mL-1 added)0.44——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (0.1 ng mL-1 row)0.1 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (0.1 ng mL-1 added)0.11 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (0.1 ng mL-1 added)1.28——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (0.1 ng mL-1 added)0.0962 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (0.1 ng mL-1 added)96.2 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (0.1 ng mL-1 added)5 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (0.1 ng mL-1 added)0.58——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (10 ng mL-1 row)10 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (10 ng mL-1 added)9.77 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (10 ng mL-1 added)1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (10 ng mL-1 added)9.88 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (10 ng mL-1 added)98.8 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (10 ng mL-1 added)4.76 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (10 ng mL-1 added)-0.033——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (1 ng mL-1 row)1 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (1 ng mL-1 added)1.03 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (1 ng mL-1 added)3.49——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (1 ng mL-1 added)0.98 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (1 ng mL-1 added)98 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (1 ng mL-1 added)3.89 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (1 ng mL-1 added)-0.25——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (50 ng mL-1 row)50 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (50 ng mL-1 added)50.29 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (50 ng mL-1 added)1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (50 ng mL-1 added)50.16 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (50 ng mL-1 added)100.3 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (50 ng mL-1 added)3.17 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (50 ng mL-1 added)0——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 added CEA concentration (5 ng mL-1 row)5 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 ELISA kit result (5 ng mL-1 added)4.89 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 F-test (5 ng mL-1 added)1.14——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 found CEA concentration (5 ng mL-1 added)5.1 ng mL-1——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 recovery (5 ng mL-1 added)102 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 RSD (5 ng mL-1 added)5.12 %——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2
Table S2 t-test (5 ng mL-1 added)0.14——SI Table
Exact Reported		26 · S7 Real sample analysis · Table S2

Selectivity, storage stability, reproducibility and operational stability tests

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

Interferents at 1 ng mL-1, CEA at 10 pg mL-1; daily storage stability over 15 days; five identical aptasensors at 0.1, 1.0 and 5.0 ng mL-1 CEA; 15 consecutive cyclic-potential cycles.

Geometry
CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE aptasensor
Context
final conductive-MOF/CTF composite sensor
Measurement source
8-9 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. 4d-f; Fig. S16
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
Operational stability RSD over consecutive cyclesMarked as a best value within this paper1.31 %——Text
Exact Reported		9 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. S16
Reproducibility RSD upper bound3.27 %——Text
Range		8 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. 4f
Reproducibility RSD lower bound2.78 %——Text
Range		8 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. 4f
Interferent concentration for selectivity test1 ng mL-1, 100 times higher than CEA (10 pg mL-1)1000 pg mL-1—Text
Exact Reported		3 · 2.3 Detection of CEA using the CTF-based ECL sensor · Fig. 4d
Selectivity against interferentsinterferents produce negligible ECL signal; CEA gives significantly enhanced response——Qualitative
Qualitative		8 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. 4d
15-day storage stability RSDMarked as a best value within this paper2.14 %——Text
Exact Reported		8 · 3.5 Selectivity, reproducibility, stability, and practicality · Fig. 4e