Other — Development of an electrochemiluminescence aptasensor combining covalent-triazine framework emitter with exonuclease III-driven DNA walker for sensitive CEA detection

Measurement evidence

Other

Development of an electrochemiluminescence aptasensor combining covalent-triazine framework emitter with exonuclease III-driven DNA walker for sensitive CEA detection · Zhang S., Li Z., Wang Y. et al. · Microchemical Journal · 2025 · 114388

1 measurement group · 3 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Native polyacrylamide gel electrophoresis (PAGE)

rHp/CuxMn3-x(HITP)2-MCH/ssDNA/CTF/AE · Electrode

12% native PAGE in 1x TAE, pH 8.0, 120 V for 20 min; ethyl bromide staining for 20 min; used to validate DNA-walker assembly.

Geometry: gel electrophoresis lanes 1-7
Context: DNA-walker aptasensor assembly validation
Measurement source: SI text · S1.8 Sodium dodecyl sulfate polyacrylamide gel electrophoresis characterization

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
PAGE validation of DNA-walker assembly	new/higher molecular-weight bands confirm dsDNA/Hp/rHp/ssDNA complexes	—	—	Qualitative Qualitative	7 · 3.3 Feasibility analysis · Fig. 3
Native PAGE run time	20 min	—	—	Text Exact Reported	SI text · S1.8 Sodium dodecyl sulfate polyacrylamide gel electrophoresis characterization
Native PAGE voltage	120 V	—	—	Text Exact Reported	SI text · S1.8 Sodium dodecyl sulfate polyacrylamide gel electrophoresis characterization