Synthesis · Nanoporous gold induced vertically standing 2D NiCo bimetal-organic framework nanosheets for non-enzymatic glucose biosensing

5 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Both

Route 1: Hydrothermal

653 · 2.2 Fabrication of 2D NiCo-MOFNs · Fig. S2

Metal precursors15 mM Ni(NO3)2.6H2O and 15 mM Co(NO3)2.6H2O

Linker precursors10 mM 2-aminoterephthalic acid

Solvents12 mL DMF/water, 1:1 volume ratio

Additivesnone reported

Atmospherenot specified; sealed Teflon autoclave

Temperature120

Time4

Substrate orientationnone; precipitate formed in solution during substrate growth

Oxidant / reductantnone reported

Work-upnot specified beyond precipitate formation

Activationnone reported

Scalability contextbulk precipitate forms as by-product under the same hydrothermal solution conditions

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O	15 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs · Fig. S2
Metal Source	Co(NO3)2.6H2O	15 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs · Fig. S2
Linker	2-aminoterephthalic acid	10 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs · Fig. S2
Solvent	DMF/water	12 mL, 1:1 volume ratio	653 · 2.2 Fabrication of 2D NiCo-MOFNs · Fig. S2

Partial recipeSource: Both

Route 2: Hydrothermal

653 · 3.1 Preparation · Fig. S5b

Metal precursorsassumed same precursor solution as 4 h NiCo-MOFNs; exact restatement not given

Linker precursors2-aminoterephthalic acid, concentration not restated for this variant

SolventsDMF/water, not restated for this variant

Additivesnone reported

Atmospherenot specified

Temperature120

Time2

Substrate orientationnanoporous gold substrate

Oxidant / reductantnone reported for MOF growth

Work-upnot specified for this variant

Activationnone reported

Scalability contextgrowth-time control

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O	15 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5b
Metal Source	Co(NO3)2.6H2O	15 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5b
Linker	2-aminoterephthalic acid	10 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5b
Solvent	DMF/water	12 mL, 1:1 volume ratio; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5b
Other	nanoporous gold microneedles	not reported	653 · 3.1 Preparation · Fig. S5b

Partial recipeSource: Both

Route 3: Hydrothermal

653 · 2.2 Fabrication of 2D NiCo-MOFNs

Metal precursors15 mM Ni(NO3)2.6H2O and 15 mM Co(NO3)2.6H2O

Linker precursors10 mM 2-aminoterephthalic acid

Solvents12 mL DMF/water, 1:1 volume ratio

Additivesnone reported for MOF growth

Atmospherenot specified; sealed Teflon autoclave

Temperature120

Time4

Substrate orientationNPG microneedles immersed in precursor solution; vertical growth induced by nanoporous gold

Oxidant / reductantnone reported for MOF growth

Work-upthoroughly washed with water; directly used as working electrode

Activationnone reported

Scalability contextbottom-up surfactant-free growth on conductive nanoporous substrate; substrate synthesis relies on prior work

Show 6 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O	15 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs
Metal Source	Co(NO3)2.6H2O	15 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs
Linker	2-aminoterephthalic acid	10 mM	653 · 2.2 Fabrication of 2D NiCo-MOFNs
Solvent	N,N-dimethylformamide (DMF)	part of 12 mL 1:1 DMF/water	653 · 2.2 Fabrication of 2D NiCo-MOFNs
Solvent	water	part of 12 mL 1:1 DMF/water	653 · 2.2 Fabrication of 2D NiCo-MOFNs
Other	nanoporous gold microneedles	not reported	653 · 2.2 Fabrication of 2D NiCo-MOFNs

Partial recipeSource: Both

Route 4: Hydrothermal

653 · 3.1 Preparation · Fig. S5d

Metal precursorsassumed same precursor solution as 4 h NiCo-MOFNs; exact restatement not given

Linker precursors2-aminoterephthalic acid, concentration not restated for this variant

SolventsDMF/water, not restated for this variant

Additivesnone reported

Atmospherenot specified

Temperature120

Time6

Substrate orientationnanoporous gold substrate

Oxidant / reductantnone reported for MOF growth

Work-upnot specified for this variant

Activationnone reported

Scalability contextgrowth-time control

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Ni(NO3)2.6H2O	15 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5d
Metal Source	Co(NO3)2.6H2O	15 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5d
Linker	2-aminoterephthalic acid	10 mM; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5d
Solvent	DMF/water	12 mL, 1:1 volume ratio; same precursor solution inferred for growth-time variant	653 · 3.1 Preparation · Fig. S5d
Other	nanoporous gold microneedles	not reported	653 · 3.1 Preparation · Fig. S5d

Vague recipeSource: Main

Route 5: Electrochemical

653 · 3.1 Preparation of vertically standing 2D NiCo-MOFNs on NPG

Metal precursorssolid gold coated stainless microneedles

Linker precursorsnone

Solventsnot specified in this paper

Additivesnot specified in this paper

Atmospherenot specified

Substrate orientationgold coated stainless microneedle substrate

Oxidant / reductantelectrochemical alloying/dealloying; details in prior work [28] not reproduced

Work-upnot specified in this paper

Activationnot specified

Scalability contextNPG is required for vertical MOFN growth but only a brief prior-work reference is provided.

Show 1 structured reagent record

Role	Reagent	Amount / concentration	Source
Other	solid gold coated stainless microneedles	not reported	653 · 3.1 Preparation of vertically standing 2D NiCo-MOFNs on NPG