Sensing Application — Self-amplifying bimetallic conductive metal-organic framework for sensitive label-free electrochemiluminescence detection of aflatoxin B1

6 measurement groups · 50 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

ECL calibration for AFB1

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

AFB1 concentration increased from 0.001 ng/mL to 100 ng/mL under optimal conditions

Geometry: label-free ECL immunosensor
Context: composite ECL biosensor
Measurement source: 6 · 3.6. ECL response and calibration curve · Fig. 6A-B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Calibration intercept	4965.21	—	—	Text Exact Reported	6 · 3.6 · Fig. 6B
AFB1 detection limitMarked as a best value within this paper	0.31 pg/mL	—	—	Text Exact Reported	6 · 3.6 · Fig. 6B
Calibration R2	R2 = 0.99	—	—	Text Exact Reported	6 · 3.6 · Fig. 6B
AFB1 calibration upper concentration	100 ng/mL	—	—	Text Exact Reported	6 · 3.6 · Fig. 6A-B
AFB1 calibration lower concentration	0.001 ng/mL	—	—	Text Exact Reported	6 · 3.6 · Fig. 6A-B
Calibration slope vs lg concentration	-718.68	—	—	Text Exact Reported	6 · 3.6 · Fig. 6B
Blank ECL intensity mean IB	IB = 7645.8	—	—	Text Exact Reported	SI text p.S4 · The calculation of detection limit
Blank ECL intensity standard deviation SB	SB = 53.1	—	—	Text Exact Reported	SI text p.S4 · The calculation of detection limit
AFB1 detection limit from SI calculation	309 fg/mL	—	—	Text Exact Reported	SI text p.S4 · The calculation of detection limit
Detection-limit signal-to-noise factor k	k = 3	—	—	Text Exact Reported	SI text p.S4 · The calculation of detection limit
Smallest detectable ECL signal IL	IL = 7486.8	—	—	Text Exact Reported	SI text p.S4 · The calculation of detection limit

standard addition recovery in commercial corn samples

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

Commercial corn samples pre-treated, spiked with AFB1 standards, and measured by the ECL sensor; SI preparation used 1 g crushed sample with 5 mL 70% methanol, shaken 4 h, centrifuged 6000 rpm for 10 min, and diluted with PBS.

Geometry: label-free ECL immunosensor
Context: composite ECL biosensor
Measurement source: 7 · 3.7. Analysis of real samples · Table 1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Initial AFB1 content in commercial corn sample	0 ng/mL	—	—	Table Exact Reported	7 · 3.7 · Table 1
Corn extraction centrifugation speed	6000 rpm for 10 min	—	—	Text Exact Reported	SI text p.S4 · Preparation of samples
Corn extraction methanol fraction	70% methanol solution	—	—	Text Exact Reported	SI text p.S4 · Preparation of samples
Corn extraction shake time	4 h	—	—	Text Exact Reported	SI text p.S4 · Preparation of samples
Corn sample average detected AFB1 after 13.5 ng/mL spike	13.1 ng/mL	—	RSD 2.56%	Table Exact Reported	7 · 3.7 · Table 1
Corn sample recovery after 13.5 ng/mL spike	97.0 %	—	RSD 2.56%	Table Exact Reported	7 · 3.7 · Table 1
Corn sample average detected AFB1 after 4.50 ng/mL spike	4.33 ng/mL	—	RSD 1.88%	Table Exact Reported	7 · 3.7 · Table 1
Corn sample recovery after 4.50 ng/mL spike	96.2 %	—	RSD 1.88%	Table Exact Reported	7 · 3.7 · Table 1
Corn sample average detected AFB1 after 9.00 ng/mL spike	8.79 ng/mL	—	RSD 1.54%	Table Exact Reported	7 · 3.7 · Table 1
Corn sample recovery after 9.00 ng/mL spikeMarked as a best value within this paper	97.7 %	—	RSD 1.54%	Table Exact Reported	7 · 3.7 · Table 1

ECL detection using CV in three-electrode system

Voltammetry

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

10 mL PBS electrolyte (1/15 M, pH 7.4, 100 mM K2S2O8), N2 injection, CV 0 to -1.6 V, scan rate 0.2 V/s, PMT 600 V, magnification 4

Atmosphere: N2-treated electrolyte
Geometry: quartz electrolytic cell; three-electrode ECL system
Context: composite ECL biosensor
Measurement source: 3 · 2.3. Detection parameters of the immunosensor

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
ECL electrolyte volume	10 mL	—	—	Text Exact Reported	3 · 2.3. Detection parameters of the immunosensor
K2S2O8 concentration in ECL electrolyte	100 mM K2S2O8	—	—	Text Exact Reported	3 · 2.3
ECL electrolyte pH	pH 7.4	—	—	Text Exact Reported	3 · 2.3. Detection parameters of the immunosensor
PBS electrolyte concentration	1/15 M	—	—	Text Exact Reported	3 · 2.3. Detection parameters of the immunosensor
PMT high voltage	600 V	—	—	Text Exact Reported	3 · 2.3
CV upper potential for ECL detection	0 V	—	—	Text Exact Reported	3 · 2.3. Detection parameters of the immunosensor
CV lower potential for ECL detection	-1.6 V	—	—	Text Exact Reported	3 · 2.3. Detection parameters of the immunosensor
CV scan rate for ECL detection	0.2 V/s	—	—	Text Exact Reported	3 · 2.3

ECL optimisation screening

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

Optimised PBS pH, K2S2O8 concentration, ZnCoMOFs concentration, and Au NPs volume

Geometry: modified glassy carbon electrode sensor
Context: composite ECL biosensor
Measurement source: 6 · 3.5. Screening of test conditions · Fig. 5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
ECL intensity at optimum Au NP volume 6 uL	about 8.1k a.u.	—	—	Figure Axis Approximate	5 · 3.5. Screening of test conditions · Fig. 5D
ECL intensity at optimum K2S2O8 100 mmol/L	about 7.6k a.u.	—	—	Figure Axis Approximate	5 · 3.5. Screening of test conditions · Fig. 5B
ECL intensity at optimum pH 7.4	about 8.6k a.u.	—	—	Figure Axis Approximate	5 · 3.5. Screening of test conditions · Fig. 5A
ECL intensity at optimum ZnCoMOFs 1 mg/mL	about 8.8k a.u.	—	—	Figure Axis Approximate	5 · 3.5. Screening of test conditions · Fig. 5C
Optimum Au NPs solution volumeMarked as a best value within this paper	6 uL	—	—	Text Exact Reported	6 · 3.5 · Fig. 5D
Optimum K2S2O8 concentrationMarked as a best value within this paper	100 mmol/L	—	—	Text Exact Reported	6 · 3.5 · Fig. 5B
Optimum PBS pHMarked as a best value within this paper	pH 7.4	—	—	Text Exact Reported	6 · 3.5 · Fig. 5A
Optimum ZnCoMOFs concentrationMarked as a best value within this paper	1 mg/mL	—	—	Text Exact Reported	6 · 3.5 · Fig. 5C

SI Table S1 analytical-performance comparison

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

Comparison table for AFB1 sensors; only the this-work row is treated as first-hand performance evidence for the ZnCoMOFs ECL sensor.

Geometry: label-free ECL immunosensor
Context: composite ECL biosensor with ZnCoMOFs component
Measurement source: S10 · Table S1 · Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
This-work Table S1 linear range upper bound	0.001-100 ng/mL	—	range	SI Table Range	S10 · Table S1 · Table S1
This-work Table S1 linear range lower bound	0.001-100 ng/mL	—	range	SI Table Range	S10 · Table S1 · Table S1
This-work Table S1 detection limitMarked as a best value within this paper	0.31 pg/mL	—	—	SI Table Exact Reported	S10 · Table S1 · Table S1
This-work Table S1 sample-preparation complexity	Moderate complexity	—	—	SI Table Qualitative	S10 · Table S1 · Table S1

stability, selectivity, and reproducibility tests

AFB1/BSA/Ab/Au NPs/ZnCoMOFs/GCE · Electrode

11 cyclic potential scans at 0.001 ng/mL AFB1; selectivity at 1 ng/mL against AFB2, AFG1, OTA, ZEN, and DON; intra-batch seven sensors and inter-batch five batches at 0.1 ng/mL AFB1

Geometry: label-free ECL immunosensor
Context: composite ECL biosensor
Measurement source: 6-7 · 3.6. ECL response and calibration curve · Fig. 6C-F

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Inter-batch reproducibility batch count	five batches	—	—	Text Exact Reported	7 · 3.6 · Fig. 6F
Intra-batch reproducibility sensor count	seven sensors	—	—	Text Exact Reported	7 · 3.6 · Fig. 6E
Reproducibility test AFB1 concentration	0.1 ng/mL	—	—	Text Exact Reported	7 · 3.6 · Fig. 6E-F
Inter-batch reproducibility RSDMarked as a best value within this paper	2.3 %	—	—	Text Exact Reported	7 · 3.6 · Fig. 6F
Selectivity test concentration	1 ng/mL each	—	—	Text Exact Reported	6 · 3.6 · Fig. 6D
AFB1 selectivity ECL intensityMarked as a best value within this paper	about 3.9k a.u.	—	visual estimate	Figure Axis Approximate	6 · 3.6 · Fig. 6D
Interferent selectivity ECL intensity range	about 7.6k-7.9k a.u. for AFB2, AFG1, OTA, ZEN, and DON	—	visual estimate	Figure Axis Approximate	6 · 3.6 · Fig. 6D
Stability over repeated scans	ECL signal intensity demonstrated relative stability after multiple scans	—	—	Text Qualitative	6 · 3.6 · Fig. 6C
Stability scan count	11 consecutive cyclic potential scans	—	—	Text Exact Reported	6 · 3.6 · Fig. 6C