Synthesis · Self-amplifying bimetallic conductive metal-organic framework for sensitive label-free electrochemiluminescence detection of aflatoxin B1

4 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

Route 1: Drop Cast

2 · 2.2. Construction of label-free ECL biosensor · Scheme 1

AdditivesAFB1 target solution

Atmosphereroom temperature

Substrate orientationBSA/Ab/Au NPs/ZnCoMOFs/GCE

Work-upelectrode dried after target binding

Show 1 structured reagent record

Role	Reagent	Amount / concentration	Source
Other	AFB1 solution	6 uL · varying concentrations	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1

Complete recipeSource: SI

Route 2: Other

SI text p.S4 · Preparation of Au NPs

Metal precursorsHAuCl4 (0.01%, 100 mL)

Solventswater (aqueous HAuCl4 solution)

Additivestrisodium citrate solution (1%, 1.5 mL)

Atmospherenot reported

Temperatureslightly boiling

Time0.05-0.067

Oxidant / reductanttrisodium citrate reductant/stabiliser

Work-upremoved from heat, cooled to room temperature; centrifuged at 13000 rpm for 5 min in the dark; supernatant collected

Activationstored in a refrigerator at 4 deg C

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	HAuCl4	100 mL · 0.01%	SI text p.S4 · Preparation of Au NPs
Reductant	trisodium citrate solution	1.5 mL · 1%	SI text p.S4 · Preparation of Au NPs
Solvent	water	Not specified	SI text p.S4 · Preparation of Au NPs

Complete recipeSource: Both

Route 3: Drop Cast

2 · 2.2. Construction of label-free ECL biosensor · Scheme 1

Metal precursorsZnCoMOFs; Au NPs

Solventsdeionized water; PBS

Additivescapture antibody Ab1; BSA

Atmosphereroom temperature drying/incubation

Time0.5 h for Au NPs incubation

Substrate orientationpolished glassy carbon electrode

Work-upGCE polished with 0.3 and 0.05 um alumina; washed with deionized water; PBS rinse after BSA blocking

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ZnCoMOFs solution	8 uL · 1 mg/mL selected as optimal ZnCoMOFs concentration	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1
Other	Au NPs	6 uL	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1
Other	capture antibody Ab1	6 uL · 1 ug/mL	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1
Additive	BSA	3 uL · 0.1 %	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1
Other	PBS	pH 7.4	2 · 2.2. Construction of label-free ECL biosensor · Scheme 1

Complete recipeSource: Both

Route 4: Solvothermal

2 · 2.1. Preparation of ZnCoMOFs

Metal precursorsZn(OAc)2.2H2O (66.2 mg); Co(OAc)2.4H2O (25 mg)

Linker precursorsHHTP (70 mg)

Solvents10 mL DMF/water (V:V = 1:1)

Atmospherenot reported

Temperature85

Time24

Work-upcentrifuged at 8500 rpm for 10 min; washed thrice

Activationvacuum dried at 60 deg C for 24 h

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Zn(OAc)2.2H2O	66.2 mg	2 · 2.1. Preparation of ZnCoMOFs
Metal Source	Co(OAc)2.4H2O	25 mg	2 · 2.1. Preparation of ZnCoMOFs
Linker	HHTP	70 mg	2 · 2.1. Preparation of ZnCoMOFs
Solvent	DMF/water	10 mL; V:V = 1:1	2 · 2.1. Preparation of ZnCoMOFs