Electrochemistry Application — Novel aptasensing strategy for efficiently quantitative analyzing Staphylococcus aureus based on defective copper-based metal–organic framework

3 measurement groups · 18 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

EIS, CV, and DPV comparison of sole-ligand Cu-MOF aptasensors

Impedance spectroscopy Voltammetry

Cu-BDC-, Cu-H3BTC-, and Cu-H4EBTC-based aptasensor controls · Electrode

Cu-BDC-, Cu-H3BTC-, Cu-H4EBTC-, and ML-Cu2O@Cu-MOF-based aptasensors compared through construction stages and S. aureus detection.

Geometry: Au electrodes modified with Cu-MOF sensing layers and aptamer
Context: pristine sole-ligand controls versus composite mixed-ligand target
Measurement source: p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3d / Table S2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
fractional aptamer coverage on Cu-BDC/AE	22.1%	0.221 fraction	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3d / Table S2
fractional aptamer coverage on Cu-H3BTC/AE	39.4%	0.394 fraction	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3d / Table S2
fractional aptamer coverage on Cu-H4EBTC/AE	46.5%	0.465 fraction	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3d / Table S2
fractional aptamer coverage on ML-Cu2O@Cu-MOF/AEMarked as a best value within this paper	around 62.8%	0.628 fraction	—	Text Approximate	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3d / Table S2
Table S2 Cu-BDC Rct values and detection DeltaRct	AE 80 ohm; modification 428.1 ohm; immobilization of Apt 549.3 ohm; theta 22.1%; detection of S. aureus 581.9 ohm; DeltaRct 32.6 ohm	32.6 ohm	—	SI Table Exact Reported	S20 / rendered SI p020 · S2. Basic characterizations / Table S2 · Table S2
Table S2 Cu-H3BTC Rct values and detection DeltaRct	AE 69.67 ohm; modification 345.37 ohm; immobilization of Apt 569.97 ohm; theta 39.4%; detection of S. aureus 815.47 ohm; DeltaRct 245.5 ohm	245.5 ohm	—	SI Table Exact Reported	S20 / rendered SI p020 · S2. Basic characterizations / Table S2 · Table S2
Table S2 Cu-H4EBTC Rct values and detection DeltaRct	AE 56.95 ohm; modification 212.58 ohm; immobilization of Apt 397.28 ohm; theta 46.5%; detection of S. aureus 747.68 ohm; DeltaRct 350.4 ohm	350.4 ohm	—	SI Table Exact Reported	S20 / rendered SI p020 · S2. Basic characterizations / Table S2 · Table S2
Table S2 ML-Cu2O@Cu-MOF Rct values and detection DeltaRctMarked as a best value within this paper	AE 75 ohm; modification 150.65 ohm; immobilization of Apt 405.25 ohm; theta 62.8%; detection of S. aureus 1254.75 ohm; DeltaRct 849.5 ohm	849.5 ohm	—	SI Table Exact Reported	S20 / rendered SI p020 · S2. Basic characterizations / Table S2 · Table S2

Cyclic voltammetry (CV) and differential pulse voltammetry (DPV)

Voltammetry

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

Construction-stage redox-current response in 0.1 M PBS containing ferri/ferrocyanide; S. aureus concentration 10 CFU mL-1 for final detection stage. SI S1.6 reports DPV -0.2 to 0.6 V, 0.01 V increment, 0.05 V amplitude, 0.05 s pulse width; CV from -0.2 to 0.8 V.

Geometry: Au electrode modified with drop-cast ML-Cu2O@Cu-MOF and aptamer
Context: composite MOF aptasensor
Measurement source: p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3b-c

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV peak current after aptamer immobilisation	49.9 uA	49.9 uA	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3c
DPV peak current of bare AE	71.4 uA	71.4 uA	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3c
DPV peak current of ML-Cu2O@Cu-MOF/AE	61.8 uA	61.8 uA	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3c
DPV peak current after S. aureus binding	33.6 uA	33.6 uA	—	Text Exact Reported	p008 / article p.8 · 3.2. Electrochemical measurements · Fig. 3c
CV measurement potential window	-0.2 to 0.8 V	—	—	Text Exact Reported	SI text · S1.6 Electrochemical measurements
DPV measurement potential window	-0.2 to 0.6 V; increment 0.01 V; amplitude 0.05 V; pulse width 0.05 s	—	—	Text Exact Reported	SI text · S1.6 Electrochemical measurements

Electrochemical impedance spectroscopy (EIS) Nyquist plots

Impedance spectroscopy

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

Frequency range 0.1 Hz to 100 kHz; amplitude 5 mV; open-circuit potential 0.22 V; 0.1 M PBS with ferri/ferrocyanide electrolyte; construction stages AE, ML-Cu2O@Cu-MOF/AE, Apt/ML-Cu2O@Cu-MOF/AE, BSA/Apt/ML-Cu2O@Cu-MOF/AE, and S. aureus/Apt/ML-Cu2O@Cu-MOF/AE.

Geometry: Au electrode modified with drop-cast ML-Cu2O@Cu-MOF and aptamer
Context: composite MOF aptasensor, with bare-AE and construction-stage comparisons
Measurement source: p006 / article p.6 · 3.2. Electrochemical measurements · Fig. 3a

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Rct of Apt/ML-Cu2O@Cu-MOF/AE	405.25 ohm	405.25 ohm	—	Text Exact Reported	p006 / article p.6 · 3.2. Electrochemical measurements · Fig. 3a
Rct of bare Au electrode	75 ohm	75 ohm	—	Text Rounded Reported	p006 / article p.6 · 3.2. Electrochemical measurements · Fig. 3a
Rct of ML-Cu2O@Cu-MOF/AE	150.65 ohm	150.65 ohm	—	Text Exact Reported	p006 / article p.6 · 3.2. Electrochemical measurements · Fig. 3a
Rct after S. aureus binding	1.25 kohm	1250 ohm	—	Text Exact Reported	p006 / article p.6 · 3.2. Electrochemical measurements · Fig. 3a