Sensing Application — Novel aptasensing strategy for efficiently quantitative analyzing Staphylococcus aureus based on defective copper-based metal–organic framework

Measurement evidence

Sensing Application

Novel aptasensing strategy for efficiently quantitative analyzing Staphylococcus aureus based on defective copper-based metal–organic framework · Tian J.-Y., Liu X., Zhang S. et al. · Food Chemistry · 2023 · 134357

5 measurement groups · 72 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

DPV calibration for S. aureus detection

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

S. aureus concentrations 10 to 1e8 CFU mL-1; 0.1 M PBS pH 7.4 containing 5 mM [Fe(CN)6]3-/4-; n = 3 error bars for calibration.

Geometry: ML-Cu2O@Cu-MOF/Au-electrode aptasensor
Context: composite MOF aptasensor
Measurement source: p007-p009 / article pp.7-9 · 3.4. Qualitative analysis of S. aureus · Fig. 4c-d

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV calibration R2	R2 = 0.996	0.996	—	Figure Axis Approximate	p007 / article p.7 · Figure 4d inset · Fig. 4d
DPV calibration slope	DeltaI = 2.4 logConS. aureus + 11.57	2.4 uA per log(CFU/mL)	—	Text Exact Reported	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4d
DPV detection range	10 to 1 x 10^8 CFU mL-1	—	—	Text Range	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4c
DPV limit of detection for S. aureusMarked as a best value within this paper	1.6 CFU mL-1	1.6 CFU/mL	—	Text Exact Reported	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4d

EIS calibration for S. aureus detection

Impedance spectroscopy

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

S. aureus concentrations 10, 100, 1000, 1e4, 1e5, 1e6, 1e7, and 1e8 CFU mL-1; 0.1 M PBS pH 7.4 containing 5 mM [Fe(CN)6]3-/4-; n = 3 error bars for calibration.

Geometry: ML-Cu2O@Cu-MOF/Au-electrode aptasensor
Context: composite MOF aptasensor
Measurement source: p007-p009 / article pp.7-9 · 3.4. Qualitative analysis of S. aureus · Fig. 4a-b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
EIS calibration R2	R2 = 0.993	0.993	—	Text Exact Reported	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4b
EIS calibration slope	DeltaRct (kohm) = 0.32 log ConS.aureus + 0.45	0.32 kohm per log(CFU/mL)	—	Text Exact Reported	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4b
EIS linear detection range	10 to 1 x 10^8 CFU mL-1	—	—	Text Range	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4b
EIS limit of detection for S. aureus	2 CFU mL-1	2 CFU/mL	—	Text Exact Reported	p009 / article p.9 · 3.4. Qualitative analysis of S. aureus · Fig. 4b

EIS and DPV optimisation

Impedance spectroscopy Voltammetry

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

Optimised ML-Cu2O@Cu-MOF dosage, aptamer concentration, S. aureus binding time, pH, and aptamer anchoring time.

Geometry: ML-Cu2O@Cu-MOF/Au-electrode aptasensor
Context: composite MOF aptasensor
Measurement source: p008 / article p.8 · 3.3. Optimization process · Fig. 3e-i

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
optimal aptamer anchoring timeMarked as a best value within this paper	1 h	1 h	—	Text Exact Reported	p008 / article p.8 · 3.3. Optimization process · Fig. 3i
optimal aptamer concentrationMarked as a best value within this paper	100 nM	100 nM	—	Text Exact Reported	p008 / article p.8 · 3.3. Optimization process · Fig. 3f
optimal ML-Cu2O@Cu-MOF dosageMarked as a best value within this paper	1 mg mL-1	1 mg/mL	—	Text Exact Reported	p008 / article p.8 · 3.3. Optimization process · Fig. 3e
optimal pH for S. aureus-aptamer bindingMarked as a best value within this paper	pH 7.4	7.4 pH	—	Text Exact Reported	p008 / article p.8 · 3.3. Optimization process · Fig. 3h
optimal S. aureus binding timeMarked as a best value within this paper	1 h	1 h	—	Text Exact Reported	p008 / article p.8 · 3.3. Optimization process · Fig. 3g

EIS and DPV recovery tests in spiked food samples

Impedance spectroscopy Voltammetry

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

Milk, honey, and biscuit samples treated according to GB4789.15-2016, spiked with S. aureus, and compared with plate count method; SI text lists Tables S3-S8 captions but not their numeric bodies.

Geometry: ML-Cu2O@Cu-MOF/Au-electrode aptasensor
Context: composite MOF aptasensor in real food matrices
Measurement source: p010 / article p.10 · 3.6. Analysis of real samples · Tables S3-S8 / Fig. S18

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV biscuit recovery range	93.3%-104.7%; RSD 0.2%-2.3%	—	RSD 0.2%-2.3%	SI Table Range	S30 / rendered SI p030 · 3.6. Analysis of real samples · Table S8
DPV biscuit recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaI 13.99 uA; found 10.19 CFU mL-1; recovery 101.9%; RSD 1.7%	101.9 % recovery	RSD 1.7%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaI 16.36 uA; found 97.72 CFU mL-1; recovery 97.7%; RSD 1.4%	97.7 % recovery	RSD 1.4%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaI 18.76 uA; found 990.38 CFU mL-1; recovery 99.0%; RSD 0.2%	99 % recovery	RSD 0.2%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaI 21.19 uA; found 10185.91 CFU mL-1; recovery 101.9%; RSD 2.3%	101.9 % recovery	RSD 2.3%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaI 23.62 uA; found 104712.91 CFU mL-1; recovery 104.7%; RSD 0.3%	104.7 % recovery	RSD 0.3%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaI 25.96 uA; found 977357.23 CFU mL-1; recovery 97.7%; RSD 1.6%	97.7 % recovery	RSD 1.6%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaI 28.31 uA; found 9440608.75 CFU mL-1; recovery 94.4%; RSD 0.3%	94.4 % recovery	RSD 0.3%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV biscuit recovery at 100000000 CFU mL-1 spike	spiked 100000000 CFU mL-1; DeltaI 30.98 uA; found 93325532.54 CFU mL-1; recovery 93.3%; RSD 1.8%	93.3 % recovery	RSD 1.8%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S8
DPV honey recovery range	94.4%-108.9%; RSD 0.2%-2.5%	—	RSD 0.2%-2.5%	SI Table Range	S30 / rendered SI p030 · 3.6. Analysis of real samples · Table S7
DPV honey recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaI 14.06 uA; found 10.89 CFU mL-1; recovery 108.9%; RSD 1.6%	108.9 % recovery	RSD 1.6%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaI 16.41 uA; found 102.33 CFU mL-1; recovery 102.3%; RSD 2.5%	102.3 % recovery	RSD 2.5%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaI 18.72 uA; found 952.8 CFU mL-1; recovery 95.3%; RSD 0.8%	95.3 % recovery	RSD 0.8%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaI 21.11 uA; found 9440.61 CFU mL-1; recovery 94.4%; RSD 2.4%	94.4 % recovery	RSD 2.4%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaI 23.55 uA; found 97723.72 CFU mL-1; recovery 97.7%; RSD 2.3%	97.7 % recovery	RSD 2.3%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaI 26.01 uA; found 1037528.45 CFU mL-1; recovery 103.8%; RSD 1.3%	103.8 % recovery	RSD 1.3%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaI 28.35 uA; found 9775162.21 CFU mL-1; recovery 97.8%; RSD 0.2%	97.8 % recovery	RSD 0.2%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV honey recovery at 100000000 CFU mL-1 spike	spiked 100000000 CFU mL-1; DeltaI 30.85 uA; found 107969228.9 CFU mL-1; recovery 108.0%; RSD 0.5%	108 % recovery	RSD 0.5%	SI Table Exact Reported	S30 / rendered SI p030 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S7
DPV milk recovery range	91.7%-109.6%; RSD 0.5%-2.8%	—	RSD 0.5%-2.8%	SI Table Range	S29 / rendered SI p029 · 3.6. Analysis of real samples · Table S6
DPV milk recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaI 14.03 uA; found 10.59 CFU mL-1; recovery 105.9%; RSD 2.8%	105.9 % recovery	RSD 2.8%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaI 16.47 uA; found 109.63 CFU mL-1; recovery 109.6%; RSD 2.1%	109.6 % recovery	RSD 2.1%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaI 18.69 uA; found 925.98 CFU mL-1; recovery 92.6%; RSD 2.4%	92.6 % recovery	RSD 2.4%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaI 21.21 uA; found 10232.93 CFU mL-1; recovery 102.3%; RSD 1.4%	102.3 % recovery	RSD 1.4%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaI 23.48 uA; found 91727.59 CFU mL-1; recovery 91.7%; RSD 1.6%	91.7 % recovery	RSD 1.6%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaI 26.08 uA; found 1096478.22 CFU mL-1; recovery 109.6%; RSD 0.5%	109.6 % recovery	RSD 0.5%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaI 28.41 uA; found 10375284.15 CFU mL-1; recovery 103.8%; RSD 0.6%	103.8 % recovery	RSD 0.6%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
DPV milk recovery at 100000000 CFU mL-1 spike	spiked 100000000 CFU mL-1; DeltaI 30.88 uA; found 109621349.62 CFU mL-1; recovery 109.6%; RSD 0.7%	109.6 % recovery	RSD 0.7%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S6
EIS biscuit recovery range	97.9%-103.2%; RSD 1.8%-4.6%	—	RSD 1.8%-4.6%	SI Table Range	S29 / rendered SI p029 · 3.6. Analysis of real samples · Table S5
EIS biscuit recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaRct 0.775 kOhm; found 10.0 CFU mL-1; recovery 100.1%; RSD 2.8%	100.1 % recovery	RSD 2.8%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaRct 1.074 kOhm; found 97.9 CFU mL-1; recovery 97.9%; RSD 4.1%	97.9 % recovery	RSD 4.1%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaRct 1.455 kOhm; found 1024.6 CFU mL-1; recovery 102.5%; RSD 2.4%	102.5 % recovery	RSD 2.4%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaRct 1.746 kOhm; found 10017.2 CFU mL-1; recovery 100.2%; RSD 4.6%	100.2 % recovery	RSD 4.6%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaRct 2.088 kOhm; found 101064.9 CFU mL-1; recovery 101.1%; RSD 1.8%	101.1 % recovery	RSD 1.8%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaRct 2.445 kOhm; found 1023440.8 CFU mL-1; recovery 102.3%; RSD 3.8%	102.3 % recovery	RSD 3.8%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS biscuit recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaRct 2.799 kOhm; found 10320796.4 CFU mL-1; recovery 103.2%; RSD 1.9%	103.2 % recovery	RSD 1.9%	SI Table Exact Reported	S29 / rendered SI p029 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S5
EIS honey recovery range	93.8%-100.2%; RSD 1.1%-3.5%	—	RSD 1.1%-3.5%	SI Table Range	S28 / rendered SI p028 · 3.6. Analysis of real samples · Table S4
EIS honey recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaRct 0.726 kOhm; found 9.4 CFU mL-1; recovery 93.8%; RSD 2.3%	93.8 % recovery	RSD 2.3%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaRct 1.094 kOhm; found 99.7 CFU mL-1; recovery 99.7%; RSD 3.3%	99.7 % recovery	RSD 3.3%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaRct 1.419 kOhm; found 999.3 CFU mL-1; recovery 99.9%; RSD 3.5%	99.9 % recovery	RSD 3.5%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaRct 1.744 kOhm; found 10005.7 CFU mL-1; recovery 100.1%; RSD 3.3%	100.1 % recovery	RSD 3.3%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaRct 2.071 kOhm; found 100242.0 CFU mL-1; recovery 100.2%; RSD 1.1%	100.2 % recovery	RSD 1.1%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaRct 2.392 kOhm; found 1001255.8 CFU mL-1; recovery 100.1%; RSD 2.8%	100.1 % recovery	RSD 2.8%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS honey recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaRct 2.71 kOhm; found 9992625.4 CFU mL-1; recovery 99.9%; RSD 3.4%	99.9 % recovery	RSD 3.4%	SI Table Exact Reported	S28 / rendered SI p028 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S4
EIS milk recovery range	91.6%-105.5%; RSD 1.8%-3.8%	—	RSD 1.8%-3.8%	SI Table Range	S27 / rendered SI p027 · 3.6. Analysis of real samples · Table S3
EIS milk recovery at 10 CFU mL-1 spike	spiked 10 CFU mL-1; DeltaRct 0.796 kOhm; found 10.3 CFU mL-1; recovery 102.8%; RSD 3.8%	102.8 % recovery	RSD 3.8%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 100 CFU mL-1 spike	spiked 100 CFU mL-1; DeltaRct 1.005 kOhm; found 91.6 CFU mL-1; recovery 91.6%; RSD 3.1%	91.6 % recovery	RSD 3.1%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 1000 CFU mL-1 spike	spiked 1000 CFU mL-1; DeltaRct 1.387 kOhm; found 976.8 CFU mL-1; recovery 97.7%; RSD 1.8%	97.7 % recovery	RSD 1.8%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 10000 CFU mL-1 spike	spiked 10000 CFU mL-1; DeltaRct 1.663 kOhm; found 9541.0 CFU mL-1; recovery 95.4%; RSD 3.7%	95.4 % recovery	RSD 3.7%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 100000 CFU mL-1 spike	spiked 100000 CFU mL-1; DeltaRct 2.168 kOhm; found 104937.1 CFU mL-1; recovery 104.9%; RSD 4.3%	104.9 % recovery	RSD 4.3%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 1000000 CFU mL-1 spike	spiked 1000000 CFU mL-1; DeltaRct 2.396 kOhm; found 1002930.1 CFU mL-1; recovery 100.3%; RSD 3.3%	100.3 % recovery	RSD 3.3%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3
EIS milk recovery at 10000000 CFU mL-1 spike	spiked 10000000 CFU mL-1; DeltaRct 2.861 kOhm; found 10549410.0 CFU mL-1; recovery 105.5%; RSD 3.8%	105.5 % recovery	RSD 3.8%	SI Table Exact Reported	S27 / rendered SI p027 · S4. Applicability of the ML-Cu2O@Cu-MOF-based aptasensor · Table S3

EIS and DPV selectivity, reproducibility, storage-stability, and regeneration tests

Impedance spectroscopy Voltammetry

Apt/ML-Cu2O@Cu-MOF/AE aptasensor · Electrode

Interferents: E. coli, Salmonella enterica, Bacillus subtilis, and Salmonella paratyphi A at 1000 CFU mL-1, plus mixture with S. aureus; reproducibility with five aptasensors; storage for 15 days at 4 C; regeneration in NaOH.

Geometry: ML-Cu2O@Cu-MOF/Au-electrode aptasensor
Context: composite MOF aptasensor
Measurement source: p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. 4e-h / Fig. S15-S17

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV regeneration RSD	0.9% over 8 runs	0.009 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. S15d
EIS regeneration RSD	4.38% over 8 runs	0.0438 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. 4h
optimal NaOH regeneration soaking timeMarked as a best value within this paper	0.1 M NaOH for 2 min	2 min	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. S16
DPV reproducibility RSD	0.24%	0.0024 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. S15b / Fig. S17
EIS reproducibility RSD	2.88%	0.0288 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. 4f
selectivity against bacterial interferents	E. coli, STM, SPA, and B. subtilis gave insignificant EIS and DPV responses; S. aureus gave dramatically high response	—	—	Text Qualitative	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. 4e / Fig. S15a
DPV storage-stability RSD over 15 days	2.66%	0.0266 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. S16c
EIS storage-stability RSD over 15 days	4.89%	0.0489 fraction	—	Text Exact Reported	p009 / article p.9 · 3.5. Selectivity, reproducibility, storage stability and regeneration ability · Fig. 4g