Synthesis · Novel aptasensing strategy for efficiently quantitative analyzing Staphylococcus aureus based on defective copper-based metal–organic framework

5 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Both

Route 1: Drop Cast

SI text · S1.3-S1.6

SolventsMilli-Q water; PBS pH 7.4; ethanol and water for electrode washing; piranha solution for Au electrode pretreatment

AdditivesS. aureus-targeted aptamer solution (100 nM); optional bovine serum albumin (BSA, 0.5%) blocking; alumina slurry (0.05 um) for polishing

AtmosphereDried under N2 during Au electrode pretreatment; otherwise ambient laboratory handling; stored at 4 C when not used.

Temperatureroom temperature

Time1

Substrate orientationpre-treated Au electrode (AE)

Work-upAu electrode polished, rinsed with piranha solution for 5 min, washed with ethanol and water for 5 min, dried under N2, electrochemically activated in 0.5 M H2SO4 from -0.2 to 1.6 V; modified electrodes rinsed with Milli-Q water and PBS after aptamer incubation.

Activation10 mg ML-Cu2O@Cu-MOF dispersed in 10 mL Milli-Q water and ultrasonically agitated for 30 min to form 1 mg/mL suspension before 10 uL drop-casting.

Scalability contextElectrode fabrication uses 10 uL aliquots on Au electrodes.

Show 10 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ML-Cu2O@Cu-MOF	10 mg dispersed in 10 mL; 10 uL drop-cast · 1 mg mL-1	SI text · S1.3-S1.6
Solvent	Milli-Q water	10 mL for MOF suspension	SI text · S1.3-S1.6
Other	S. aureus-targeted aptamer	incubated for 1 h; sequence reported in SI S1.1 · 100 nM for aptasensor fabrication; stock 1 uM	SI text · S1.3-S1.6
Other	bovine serum albumin (BSA)	optional blocking step · 0.5%	SI text · S1.3-S1.6
Other	Au electrode	3 mm diameter	SI text · S1.3-S1.6
Other	alumina slurry	used for polishing · 0.05 um	SI text · S1.3-S1.6
Acid	piranha solution (H2O2 and H2SO4)	rinsed for 5 min · H2O2:H2SO4 = 3:7 v/v in S1.3; S1.6 also reports VH2SO4/VH2O2 = 7:3	SI text · S1.3-S1.6
Acid	H2SO4	electrochemical activation from -0.2 V to 1.6 V · 0.5 M	SI text · S1.3-S1.6
Electrolyte	PBS	0.242 g KH2PO4, 1.445 g Na2HPO4*12H2O, 0.2 g KCl, 8.003 g NaCl in 1.0 L Milli-Q water · 0.1 M PBS used for experiments	SI text · S1.3-S1.6
Electrolyte	K3Fe(CN)6/K4Fe(CN)6 in PBS	1.65 g K3Fe(CN)6 and 2.111 g K4Fe(CN)6 in 1 L PBS · 5 mM [Fe(CN)6]3-/4- in measurements	SI text · S1.3-S1.6

Partial recipeSource: Both

Route 2: Solvothermal

SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Metal precursorsCu(NO3)2; amount not explicitly reported for this sole-ligand control in this paper

Linker precursorsH2BDC / BDC sole ligand; paper does not report exact sole-ligand amount

SolventsMain text says similar way to ML-Cu2O@Cu-MOF; exact solvent quantities for this control are not explicitly reproduced

AdditivesPVP-K30 likely by similar route; exact control amount not explicitly reproduced

AtmosphereNot stated; likely sealed autoclave by similar route

Temperature120

Time36

Work-upMain text says prepared by a similar way; exact workup for this control not separately reproduced

ActivationMain text says similar way; exact drying/activation for this control not separately reproduced

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	H2BDC / terephthalic acid	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC
Metal Source	Cu(NO3)2	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Partial recipeSource: Both

Route 3: Solvothermal

SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Metal precursorsCu(NO3)2; amount not explicitly reported for this sole-ligand control in this paper

Linker precursorsH3BTC sole ligand; paper does not report exact sole-ligand amount

SolventsMain text says similar way to ML-Cu2O@Cu-MOF; exact solvent quantities for this control are not explicitly reproduced

AdditivesPVP-K30 likely by similar route; exact control amount not explicitly reproduced

AtmosphereNot stated; likely sealed autoclave by similar route

Temperature120

Time36

Work-upMain text says prepared by a similar way; exact workup for this control not separately reproduced

ActivationMain text says similar way; exact drying/activation for this control not separately reproduced

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	H3BTC / 1,3,5-benzenetricarboxylic acid	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC
Metal Source	Cu(NO3)2	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Partial recipeSource: Both

Route 4: Solvothermal

SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Metal precursorsCu(NO3)2; amount not explicitly reported for this sole-ligand control in this paper

Linker precursorsH4EBTC sole ligand; paper does not report exact sole-ligand amount

SolventsMain text says similar way to ML-Cu2O@Cu-MOF; exact solvent quantities for this control are not explicitly reproduced

AdditivesPVP-K30 likely by similar route; exact control amount not explicitly reproduced

AtmosphereNot stated; likely sealed autoclave by similar route

Temperature120

Time36

Work-upMain text says prepared by a similar way; exact workup for this control not separately reproduced

ActivationMain text says similar way; exact drying/activation for this control not separately reproduced

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Linker	H4EBTC / diphenylethyne-3,3',5,5'-tetracarboxylic acid	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC
Metal Source	Cu(NO3)2	not explicitly reported for control synthesis in this paper	SI text · S1.2 Synthesis of Cu-BDC, Cu-H3BTC, and Cu-H4EBTC

Complete recipeSource: Both

Route 5: Solvothermal

p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF

Metal precursorsCu(NO3)2 (60 mg, 0.32 mmol)

Linker precursorsH2BDC (16.6 mg), H3BTC (21 mg), H4EBTC (50.6 mg)

Solvents10 mL dichloromethane/methanol, volume ratio 1:1

Additivespolyvinylpyrrolidone-K30 (150 mg)

AtmosphereNot stated; sealed Teflon-lined autoclave.

Temperature120

Time36

Oxidant / reductantNo separate reductant reported; partial Cu2+ reduction to Cu2O inferred during preparation.

Work-upProduct obtained after centrifuging/centrifugalizing and washing.

ActivationDried in vacuum at 60 C for 8 h.

Scalability contextBatch quantities reported at tens to hundreds of milligrams; yield not reported.

Show 7 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Cu(NO3)2	60 mg, 0.32 mmol; purchased from Tianjin Kemiou Chemical Reagent Corporation	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Additive	polyvinylpyrrolidone-K30	150 mg; PVP-K30 purchased from Beijing Solarbio Science & Technology Corporation	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Solvent	dichloromethane	part of 10 mL mixed solvent, 1:1 v/v with methanol	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Solvent	methanol	part of 10 mL mixed solvent, 1:1 v/v with dichloromethane	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Linker	H2BDC / terephthalic acid	16.6 mg; H2BDC purchased from Aladdin Instrument Corporation	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Linker	H3BTC / 1,3,5-benzenetricarboxylic acid	21 mg; H3BTC purchased from Aladdin Instrument Corporation	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF
Linker	H4EBTC / diphenylethyne-3,3',5,5'-tetracarboxylic acid	50.6 mg; H4EBTC supplied by Chemsoon Chemical technology Corporation	p003 / article p.3 · 2.1. Synthesis of ML-Cu2O@Cu-MOF