Electrochemistry Application — Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon

3 measurement groups · 12 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

cyclic voltammetry

Voltammetry

Au NPs@ZIF-8/OMC/GCE · Electrode

CV in 0.1 M KCl containing 5 mM Fe(CN)6(3-/4-) at 50 mV s-1, comparing Au@ZIF-8/OMC/GCE, bare GCE, Au@ZIF-8/GCE, ZIF-8/GCE, anti-CEA/Au@ZIF-8/OMC/GCE and CEA/anti-CEA/Au@ZIF-8/OMC/GCE.

Geometry: Three-electrode cell with modified GCE working electrode, SCE reference, Pt plate auxiliary.
Context: Composite platform compared with pristine ZIF-8 and electrode controls.
Measurement source: 4-5 · Characteristics of different electrodes · Fig. 4a

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Au@ZIF-8/OMC/GCE anodic peak potential	0.307 V	—	—	Text Exact Reported	5 · Characteristics of different electrodes · Fig. 4a
Au@ZIF-8/OMC/GCE cathodic peak potential	0.234 V	—	—	Text Exact Reported	5 · Characteristics of different electrodes · Fig. 4a
Au@ZIF-8/OMC/GCE peak-to-peak separation	around 73 mV	—	—	Text Approximate	5 · Characteristics of different electrodes · Fig. 4a
OMC enhances electrochemical currentMarked as a best value within this paper	Au NPs@ZIF-8/OMC/GCE peak current larger than Au NPs@ZIF-8/GCE	—	—	Text Qualitative	4 · Characteristics of different electrodes · Fig. 4a
ZIF-8 decreases electrode peak current	peak current obviously decreased after ZIF-8 was dropped on an electrode, ascribed to nonconducting properties of ZIF-8	—	—	Text Qualitative	4 · Characteristics of different electrodes · Fig. 4a

differential pulse voltammetry

Voltammetry

Au NPs@ZIF-8/OMC/GCE · Electrode

DPV current change (Delta I_DPV) for 100 ng mL-1 CEA using electrodes modified by ZIF-8, Au@ZIF-8, OMC, ZIF-8/OMC and Au@ZIF-8/OMC in 0.1 M KCl containing 5 mM Fe(CN)6(3-/4-), n = 3.

Geometry: Modified GCE electrodes.
Context: Material-component comparison including pristine ZIF-8 control.
Measurement source: 5-6 · Characteristics of different electrodes · Fig. 4c

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV current change for Au@ZIF-8 electrode	approximately 1.4 x 10-5 A	—	—	Visual Estimate Approximate	5 · Characteristics of different electrodes · Fig. 4c
DPV current change for Au@ZIF-8/OMC electrodeMarked as a best value within this paper	approximately 2.7 x 10-5 A	—	—	Visual Estimate Approximate	6 · Characteristics of different electrodes · Fig. 4c
DPV current change for OMC electrode	approximately 0.7 x 10-5 A	—	—	Visual Estimate Approximate	5 · Characteristics of different electrodes · Fig. 4c
DPV current change for ZIF-8 electrode	approximately 0.85 x 10-5 A	—	—	Visual Estimate Approximate	5 · Characteristics of different electrodes · Fig. 4c
DPV current change for ZIF-8/OMC electrode	approximately 1.6 x 10-5 A	—	—	Visual Estimate Approximate	5 · Characteristics of different electrodes · Fig. 4c

electrochemical impedance spectroscopy

Impedance spectroscopy

Au NPs@ZIF-8/OMC/GCE · Electrode

EIS measured from 100 kHz to 1 Hz with signal amplitude of 5 mV for electrode modification series.

Geometry: Three-electrode cell with modified GCE working electrode, SCE reference, Pt plate auxiliary.
Context: Au@ZIF-8/OMC/GCE compared against ZIF-8/GCE, Au@ZIF-8/GCE, bare GCE and biofunctionalised electrodes.
Measurement source: 5-6 · Characteristics of different electrodes · Fig. 4b

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Au@ZIF-8 lowers electron-transfer resistance vs ZIF-8	Au@ZIF-8 modified GCE presented smaller electron transfer resistance than ZIF-8 modified GCE	—	—	Text Qualitative	5 · Characteristics of different electrodes · Fig. 4b
Protein and immunocomplex layers increase electron-transfer resistance	immobilization of antibody and combination between CEA and anti-CEA lead to increased electron transfer resistance	—	—	Text Qualitative	6 · Characteristics of different electrodes · Fig. 4b