Synthesis · Electrochemical immunoassay for the carcinoembryonic antigen based on Au NPs modified zeolitic imidazolate framework and ordered mesoporous carbon

3 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Complete recipeSource: Main

3 · Synthesis of Au@ZIF-8 nanoparticles

Metal precursorsZIF-8 powder, 100 mg; chloroauric acid methanol solution, 426 uL, 0.1 g mL-1

Linker precursorsZIF-8 framework linker already present

Solventsmethanol

Atmospherenot reported

Temperatureroom temperature for reduction; 50 C drying

Time30 min ultrasonication; 1 h stirring after NaBH4 addition

Oxidant / reductantNaBH4 methanol solution, 2.5 mL, 0.01 M

Work-upCentrifuged to collect light purple precipitate; separated; washed several times with methanol.

ActivationDried in a vacuum oven under 50 C.

Show 4 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	ZIF-8 powder	100 mg	3 · Synthesis of Au@ZIF-8 nanoparticles
Metal Source	chloroauric acid	426 uL · 0.1 g mL-1 in methanol	3 · Synthesis of Au@ZIF-8 nanoparticles
Reductant	NaBH4	2.5 mL · 0.01 M in methanol	3 · Synthesis of Au@ZIF-8 nanoparticles
Solvent	methanol	Not specified	3 · Synthesis of Au@ZIF-8 nanoparticles

Complete recipeSource: Main

3 · Fabrication of immunosensor · Fig. 1

Metal precursorsAu@ZIF-8 methanol solution, 3 uL, 2 mg mL-1

Linker precursorsZIF-8 framework linker already present

Solventsmethanol; deionised water; ethanol; acetone; PBS

Additivesanti-CEA antibodies; BSA; CEA

Atmospherenot reported

Temperatureroom temperature drying; 4 C antibody immobilisation; 37 C CEA incubation

TimeGCE sonication 3 min; anti-CEA at least 12 h; BSA 1 h; CEA 40 min

Substrate orientation3 mm diameter glassy carbon electrode

Work-upGCE polished with 0.05 um Al2O3 slurry; sonicated in water, ethanol and acetone; PBS washing after antibody and antigen steps.

Scalability contextSmall-volume drop-cast electrode fabrication.

Show 5 structured reagent records

Role	Reagent	Amount / concentration	Source
Additive	ordered mesoporous carbon	0.25 mg dispersed in 1 mL methanol; 3 uL dropped · 0.25 mg mL-1	3 · Fabrication of immunosensor · Fig. 1
Additive	Au@ZIF-8 methanol solution	3 uL · 2 mg mL-1	3 · Fabrication of immunosensor · Fig. 1
Other	anti-CEA antibodies	10 uL · optimised loading 20 ug mL-1	3 · Fabrication of immunosensor · Fig. 1
Other	BSA	1%	3 · Fabrication of immunosensor · Fig. 1
Other	CEA	3 uL · various concentrations	3 · Fabrication of immunosensor · Fig. 1

Complete recipeSource: Main

3 · Synthesis of ZIF-8

Metal precursorsZn(NO3)2.6H2O, 40 mL methanol solution, 0.1 mol L-1

Linker precursors2-methylimidazole, 40 mL methanol solution, 0.1 mol L-1

Solventsmethanol

Atmospherenot reported

Temperatureroom temperature for synthesis; 50 C drying

Time1 h stirring

Work-upCentrifuged; repeatedly washed with methanol; white precipitate collected.

ActivationDried in a vacuum oven under 50 C.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Zn(NO3)2.6H2O	40 mL solution · 0.1 mol L-1 in methanol	3 · Synthesis of ZIF-8
Linker	2-methylimidazole	40 mL solution · 0.1 mol L-1 in methanol	3 · Synthesis of ZIF-8
Solvent	methanol	Not specified	3 · Synthesis of ZIF-8