Sensing Application — TCNQ-doped Cu-metal organic framework as a novel conductometric immunosensing platform for the quantification of prostate cancer antigen

3 measurement groups · 58 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Conductometric PSA sensing by surface conductance change

antibody-TCNQ-Cu3(BTC)2-SPE · Electrode

100 uL antigen sample loaded on sensor surface and incubated for 20 min; all measurements at 25 +/- 2 C; averages from triplicate readings.

Temperature: 298
Atmosphere: PBS or spiked serum
Geometry: antibody-TCNQ-Cu3(BTC)2 SPE conductometric immunosensor
Context: biofunctionalised conductive MOF sensing electrode
Measurement source: 3 · 2.4 Conductometric sensing of PSA · Fig. 6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Blank-response standard deviation used for LOD	Sa is ... 0.03	—	—	Text Exact Reported	3 · 2.4 Conductometric sensing of PSA
Log-log PSA calibration intercept	y = -0.1863x + 0.066	—	—	Figure Axis Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6B
Log-log PSA calibration R2Marked as a best value within this paper	R2 = 0.9944	—	—	Figure Axis Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6B
Log-log PSA calibration slope	y = -0.1863x + 0.066	—	—	Figure Axis Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6B
Measurement temperature	25 +/- 2 C	298.15 K	+/- 2 C	Text Exact Reported	3 · 2.4 Conductometric sensing of PSA
PSA analysis concentration range upper bound tested	0.01-150 ng/mL	—	—	Text Range	4 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6
PSA analysis concentration range lower bound tested	0.01-150 ng/mL	—	—	Text Range	4 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6
Logarithmically linear PSA detection range upper boundMarked as a best value within this paper	0.1-100 ng/mL	—	—	Text Range	4 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6
Logarithmically linear PSA detection range lower bound	0.1-100 ng/mL	—	—	Text Range	4 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6
PSA limit of detectionMarked as a best value within this paper	0.06 ng/mL	—	—	Text Exact Reported	5 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. 6
PSA immunosensor sensitivity	1.5 mA/ppb	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 1
PSA sample incubation time	20 min	—	—	Text Exact Reported	3 · 2.4 Conductometric sensing of PSA
PSA sample volume loaded	100 uL	—	—	Text Exact Reported	3 · 2.4 Conductometric sensing of PSA
Replicate count for reported measurements	average calculated from triplicate readings	—	—	Text Exact Reported	3 · 2.4 Conductometric sensing of PSA

Conductometric analysis of spiked serum samples

antibody-TCNQ-Cu3(BTC)2-SPE · Electrode

Spiked serum conductance corrected by baseline adjustment against standard buffer calibration.

Temperature: 298
Atmosphere: spiked serum
Geometry: antibody-TCNQ-Cu3(BTC)2 SPE
Context: biofunctionalised conductive MOF sensing electrode
Measurement source: 6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Standard-buffer conductance for 10 ng/mL PSA	0.76 +/- 0.008 mS	—	+/- 0.008 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Serum-buffer conductance difference for 10 ng/mL PSA	0.40 mS	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Estimated PSA for 10 ng/mL serum spike	9.86 ng/mL	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Observed conductance for 10 ng/mL PSA in serum	0.36 +/- 0.008 mS	—	+/- 0.008 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Recovery for 10 ng/mL serum spike	98.6%	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Standard-buffer conductance for 1 ng/mL PSA	1.17 +/- 0.005 mS	—	+/- 0.005 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Serum-buffer conductance difference for 1 ng/mL PSA	0.40 mS	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Estimated PSA for 1 ng/mL serum spike	1.02 ng/mL	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Observed conductance for 1 ng/mL PSA in serum	0.77 +/- 0.006 mS	—	+/- 0.006 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Recovery for 1 ng/mL serum spike	102%	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Standard-buffer conductance for 20 ng/mL PSA	0.66 +/- 0.007 mS	—	+/- 0.007 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Serum-buffer conductance difference for 20 ng/mL PSA	0.39 mS	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Estimated PSA for 20 ng/mL serum spike	19.4 ng/mL	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Observed conductance for 20 ng/mL PSA in serum	0.27 +/- 0.007 mS	—	+/- 0.007 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Recovery for 20 ng/mL serum spike	97%	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Standard-buffer conductance for 50 ng/mL PSA	0.56 +/- 0.005 mS	—	+/- 0.005 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Serum-buffer conductance difference for 50 ng/mL PSA	0.40 mS	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Estimated PSA for 50 ng/mL serum spike	50.8 ng/mL	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Observed conductance for 50 ng/mL PSA in serum	0.16 +/- 0.005 mS	—	+/- 0.005 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Recovery for 50 ng/mL serum spike	102%	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Standard-buffer conductance for 5 ng/mL PSA	0.87 +/- 0.006 mS	—	+/- 0.006 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Serum-buffer conductance difference for 5 ng/mL PSA	0.41 mS	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Estimated PSA for 5 ng/mL serum spike	5.1 ng/mL	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Observed conductance for 5 ng/mL PSA in serum	0.46 +/- 0.006 mS	—	+/- 0.006 mS	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2
Recovery for 5 ng/mL serum spike	102%	—	—	Table Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Table 2

Operational stability, intra-assay precision, inter-assay precision, response-time and regeneration tests

antibody-TCNQ-Cu3(BTC)2-SPE · Electrode

10 ng/mL PSA specificity, storage stability, batch variation, response-time and regeneration tests; rendered SI Fig. S5-S8 inspected.

Temperature: 298
Atmosphere: PBS/PSA solutions
Geometry: antibody-TCNQ-Cu3(BTC)2 SPE
Context: biofunctionalised conductive MOF sensing electrode
Measurement source: 6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S5-S8

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Glycine-HCl regeneration concentration	10 mM	—	—	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs
Number of independently prepared electrodes for inter-assay precision	four sensor electrodes	—	—	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S7
Inter-assay sensor-response variation	+/-3%	—	+/-3%	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S7
Intra-assay response stability over storage	+/-5% over a period of 4 weeks	—	+/-5%	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S6
Signal attenuation after recycling	< 10%	—	—	Text Approximate	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs
Recycling steps with retained output	up to the five recycling steps	—	—	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs
Fast response capabilityMarked as a best value within this paper	2 min	—	—	Text Exact Reported	6 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S8
Specificity 10 ng/mL avidin response from SI Fig. S5	about 2.7 mS	—	—	Visual Estimate Approximate	6 · Supplementary information · Fig. S5
Specificity blank response from SI Fig. S5	about 2.7 mS	—	—	Visual Estimate Approximate	6 · Supplementary information · Fig. S5
Specificity 10 ng/mL BSA response from SI Fig. S5	about 2.65 mS	—	—	Visual Estimate Approximate	6 · Supplementary information · Fig. S5
Specificity 10 ng/mL PSA response from SI Fig. S5	about 0.75 mS	—	—	Visual Estimate Approximate	6 · Supplementary information · Fig. S5
Stored sensor conductance at day 0 from SI Fig. S6	about 0.75 mS	—	—	Visual Estimate Approximate	7 · Supplementary information · Fig. S6
Stored sensor conductance at day 28 from SI Fig. S6	about 0.70 mS	—	—	Visual Estimate Approximate	7 · Supplementary information · Fig. S6
Stored sensor conductance at day 40 from SI Fig. S6	about 0.68 mS	—	—	Visual Estimate Approximate	7 · Supplementary information · Fig. S6
Batch-wise conductance visual maximum from SI Fig. S7	about 0.77 mS	—	—	Visual Estimate Approximate	8 · Supplementary information · Fig. S7
Batch-wise conductance visual minimum from SI Fig. S7	about 0.74 mS	—	—	Visual Estimate Approximate	8 · Supplementary information · Fig. S7
Response-time conductance at 2 min from SI Fig. S8	about 0.75 mS	—	—	Visual Estimate Approximate	9 · Supplementary information · Fig. S8
Response-time conductance plateau from SI Fig. S8	about 0.76 mS	—	—	Visual Estimate Approximate	9 · Supplementary information · Fig. S8
Response to non-specific proteins	no response to bovine serum albumin and avidin	—	—	Text Qualitative	5 · 3.5 Application of antibody-TCNQ-Cu3(BTC)2 SPEs · Fig. S5