Synthesis · TCNQ-doped Cu-metal organic framework as a novel conductometric immunosensing platform for the quantification of prostate cancer antigen

3 structured synthesis routes

Completeness describes how fully the route could be reconstructed from the main article and supporting information.

Partial recipeSource: Main

2 · 2.3 Immobilization of anti-PSA antibodies on TCNQ-Cu3(BTC)2

Metal precursorsTCNQ-Cu3(BTC)2-SPE

SolventsPBS buffer pH 7.2

Additivesanti-PSA antibody; Tween 20 blocking agent

Atmospherenot specified

Time1

Substrate orientation10 uL antibody solution incubated on electrode surface

Work-upWashed with PBS after antibody immobilisation and after Tween 20 blocking; stored refrigerated at 4 C.

Scalability contextSurface functionalisation recipe for individual SPEs; no scale-up reported.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Other	polyclonal anti-PSA antibodies (biological Source: rabbit)	10 uL · 1 ug/mL	2 · 2.3 Immobilization of anti-PSA antibodies on TCNQ-Cu3(BTC)2
Solvent	PBS buffer at pH 7.2	Not specified	2 · 2.3 Immobilization of anti-PSA antibodies on TCNQ-Cu3(BTC)2
Additive	Tween 20 in PBS	0.01%	2 · 2.3 Immobilization of anti-PSA antibodies on TCNQ-Cu3(BTC)2

Partial recipeSource: Main

2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ

Metal precursors0.5 mM Cu(OAc)2.H2O solution

Linker precursors0.5 mM ethanolic H3BTC solution

Solventsethanol for H3BTC solution; metal-solution solvent not specified in main text

Atmospherenot specified

Temperature190

Time1.5

Substrate orientationworking area of pre-cleaned gold SPE placed in contact with linker solution, then dipped in copper acetate solution

Work-upTen subsequent dipping sessions in linker and metal ion solutions; modified SPEs dried at 190 C for 90 min.

ActivationDried/heated at 190 C for 90 min to remove coordinated water molecules.

Scalability contextSequential dipping on 7.1 mm2 screen printed electrodes; no batch yield or film thickness reported.

Show 3 structured reagent records

Role	Reagent	Amount / concentration	Source
Metal Source	Copper acetate [Cu(OAc)2.H2O]	0.5 mM	2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ
Linker	trimesic acid (H3BTC) (98%)	0.5 mM	2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ
Solvent	ethanol	Not specified	2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ

Partial recipeSource: Main

2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ

Metal precursorspreformed Cu3(BTC)2-SPE

Linker precursorsBTC in preformed Cu3(BTC)2

SolventsCH2Cl2

AdditivesTCNQ

Atmospherevacuum drying after treatment; soaking atmosphere not specified

Timeup to 48

Substrate orientationmodified SPE soaked in saturated TCNQ solution

Oxidant / reductantTCNQ redox-active charge-transfer dopant; no external oxidant/reductant

Work-upAfter treatment, TCNQ-MOF-modified SPEs dried under vacuum conditions.

Scalability contextSoaking time controls conductance; SI Table S1 incubation-time values were read from the rendered SI.

Show 2 structured reagent records

Role	Reagent	Amount / concentration	Source
Additive	7,7,8,8-tetracyanoquinodimethane (TCNQ)	saturated solution	2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ
Solvent	dichloromethane (CH2Cl2)	Not specified	2 · 2.2 Synthesis of Cu3(BTC)2 thin films and their doping with TCNQ