Sensing Application — Electrochemical deposition of Cu metal-organic framework films for the dual analysis of pathogens

8 measurement groups · 27 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE biosensor · Electrode

Optimisation of DNA aptamer concentration and target incubation times.

Temperature: 310
Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.5, article p.8999 · Optimization of Experimental Conditions · Figures S3-S4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
optimal aptamer concentration for cell detectionMarked as a best value within this paper	0.25 uM	—	—	Text Exact Reported	main p.5, article p.8999 · Optimization of Experimental Conditions · Figure S4A
optimal aptamer concentration for supernatant detectionMarked as a best value within this paper	1 uM	—	—	Text Exact Reported	main p.5, article p.8999 · Optimization of Experimental Conditions · Figure S3A
optimal cell reaction timeMarked as a best value within this paper	30 min	0.5 h	—	Text Exact Reported	main p.5, article p.8999 · Optimization of Experimental Conditions · Figure S4B
optimal supernatant incubation timeMarked as a best value within this paper	15 min	0.25 h	—	Text Exact Reported	main p.5, article p.8999 · Optimization of Experimental Conditions · Figure S3B

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE after S. aureus cell capture · Electrode

Direct S. aureus cell detection over the same concentration series.

Temperature: 310
Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.5, article p.8999 · Performance of the Biosensor · Figure 4C,D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
cell calibration equation	y = 47.135 - 4.99x, r2 = 0.996	—	—	Text Exact Reported	main p.6, article p.8999 · Performance of the Biosensor · Figure 4D
cell detection limitMarked as a best value within this paper	5.2 cfu/mL	—	—	Text Exact Reported	main p.6, article p.8999 · Performance of the Biosensor · Figure 4D
cell calibration lower limit	7 cfu/mL	—	—	Caption Exact Reported	main p.5, article p.8998 · Figure caption · Figure 4
cell calibration upper limit	7 x 10^6 cfu/mL	—	—	Caption Exact Reported	main p.5, article p.8998 · Figure caption · Figure 4

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE after S. aureus supernatant exposure · Electrode

10 uL S. aureus supernatant; concentration series 0, 7, 7e1, 7e2, 7e3, 7e4, 7e5 and 7e6 cfu/mL.

Temperature: 310
Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.5, article p.8998-p.8999 · Performance of the Biosensor · Figure 4A,B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
supernatant calibration equation	y = 9.22 + 5.95x, r2 = 0.995	—	—	Text Exact Reported	main p.5, article p.8999 · Performance of the Biosensor · Figure 4B
supernatant detection limitMarked as a best value within this paper	1.9 cfu/mL	—	—	Text Exact Reported	main p.5, article p.8999 · Performance of the Biosensor · Figure 4B
supernatant calibration lower limit	7 cfu/mL	—	—	Text Exact Reported	main p.5, article p.8999 · Performance of the Biosensor · Figure 4B
supernatant calibration upper limit	7 x 10^6 cfu/mL	—	—	Text Exact Reported	main p.5, article p.8999 · Performance of the Biosensor · Figure 4B
SI comparison proposed-method linear range	7 - 7 x 10^6 CFU/mL	—	—	SI Table Exact Reported	SI p.7 · Table S1 · Table S1

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE after S. aureus cell capture · Electrode

S. aureus cells spiked in urine; n = 3.

Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.6, article p.8999 · Real-Sample Analysis · Table 1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
urine cell recovery at 70 cfu/mL added	103%	—	RSD 3.74%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1
urine cell recovery at 7 x 10^3 cfu/mL added	106%	—	RSD 4.83%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1
urine cell recovery at 7 x 10^5 cfu/mL added	96.7%	—	RSD 4.57%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE after S. aureus supernatant exposure · Electrode

S. aureus supernatant spiked in urine; n = 3.

Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.6, article p.8999 · Real-Sample Analysis · Table 1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
urine supernatant detected concentration at 70 cfu/mL added	6.81 +/- 0.24 x 10 cfu/mL	—	+/- 2.4 cfu/mL	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1
urine supernatant recovery at 70 cfu/mL added	97.2%	—	RSD 3.52%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1
urine supernatant recovery at 7 x 10^3 cfu/mL added	102%	—	RSD 4.60%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1
urine supernatant recovery at 7 x 10^5 cfu/mL added	97.8%	—	RSD 4.67%	Table Exact Reported	main p.6, article p.8999 · Real-Sample Analysis · Table 1

electrochemical impedance spectroscopy

Impedance spectroscopy

DNA/AuNPs/Cu-MOF/GCE after S. aureus cell capture · Electrode

EIS response after target supernatant or S. aureus cell incubation.

Temperature: 310
Geometry: aptamer-modified three-electrode biosensor
Context: composite application
Measurement source: main p.4, article p.8998 · Feasibility of the Biosensor · Figure 3A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
EIS resistance after S. aureus cell capture	almost reached 1500 ohm	—	—	Text Approximate	main p.4, article p.8998 · Feasibility of the Biosensor · Figure 3A
EIS resistance after S. aureus supernatant	about 90 ohm from Figure 3A	—	—	Figure Axis Approximate	main p.4, article p.8998 · Feasibility of the Biosensor · Figure 3A

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE biosensor · Electrode

Selectivity against E. coli and L. monocytogenes supernatants/cells at 7 x 10^3 cfu/mL in PBS.

Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: main p.6, article p.8999 · Performance of the Biosensor · Figure S5

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
selectivity current for E. coli supernatant	about 33 uA from Figure S5	—	—	Figure Axis Approximate	SI p.6 · Figure S5 caption · Figure S5
selectivity current for S. aureus cells	about 14 uA from Figure S5	—	—	Figure Axis Approximate	SI p.6 · Figure S5 caption · Figure S5
selectivity current for S. aureus supernatant	about 54 uA from Figure S5	—	—	Figure Axis Approximate	SI p.6 · Figure S5 caption · Figure S5

differential pulse voltammetry

Voltammetry

DNA/AuNPs/Cu-MOF/GCE biosensor · Electrode

Specificity after incubating samples at 100 deg C for 20 min; Exo1, DNase1, S. aureus supernatant/MNase and mixture in urine.

Temperature: 373
Geometry: DNA/AuNPs/Cu-MOF/GCE biosensor
Context: composite application
Measurement source: SI p.6 · Figure S6 caption · Figure S6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
DPV current change for Exo1 after heating	about 1.7 uA from Figure S6E	—	—	Figure Axis Approximate	SI p.6 · Figure S6 caption · Figure S6E
DPV current change for S. aureus supernatant/MNase after heating	about 20.5 uA from Figure S6E	—	—	Figure Axis Approximate	SI p.6 · Figure S6 caption · Figure S6E