Wang Y., Feng H., Huang K. et al. · Biosensors and Bioelectronics · 2022 · 114572
Open a family to keep every result attached to its sample, method and conditions.
Paraphrased for this database from the authors’ stated interpretations — never quoted verbatim — and kept separate from reported measurements.
Dual independent Fc and MB readouts improve reliability over single-signal variants.
Caveat: Single-signal LODs are close to dual-signal LODs; the accuracy/reliability advantage is mainly based on redundant readout and author interpretation.
main p.6 · 3.4 Analytical performance · Fig. 6; Fig. S3-S8 · Linked to 4 structured results
The dual-signal HCR biosensor detects miRNA-21 over 5 to 1.0 x 10^4 fM with fM-level LODs.
Caveat: Optimisation and comparison tables in SI were unreadable, so only main-text analytical figures are extracted.
main p.1, p.6 · Abstract; 3.4 · Fig. 6A,B · Linked to 5 structured results
The T4-DNA/AuNPs/N-PCD platform can be adapted for different miRNA targets by replacing the tetrahedral recognition sequence and signal probe.
Caveat: Demonstrated for miRNA-21/miRNA-141 in SI; broader target generality is prospective.
SI p.7 · Analytical performance of biosensors · Fig. S7; Fig. S8 · Linked to 2 structured results
N-PCD immobilisation amplifies both Fc and MB DPV signals compared with the no-N-PCD electrode.
Caveat: Application-specific DPV response at 2000 fM miRNA-21, not a general transport value.
main p.5 · 3.3 Electrochemical study · Fig. 4D · Linked to 2 structured results
The biosensor can detect miRNA-21 in cell RNA and serum samples with recoveries from 92.16% to 106.57%.
Caveat: Clinical sample count is small; serum samples are diluted and spiked for recovery assessment.
main p.7 · 3.4 Analytical performance · Table S4; Fig. 6D · Linked to 3 structured results
The dual-signal sensor is selective for miRNA-21 against related miRNA and mutant RNA interferents at 2000 fM.
Caveat: Reported qualitatively; individual interferent current values were not tabulated.
main p.6 · 3.4 Analytical performance · Fig. 6C · Linked to 1 structured result
The paper reports electrochemical evidence for improved electron transfer but does not report bulk electrical-transport or thermoelectric measurements for the MOF-derived carbon.
Caveat: Statement based on complete main text read; unreadable SI might contain method details but the main article frames all conductivity claims through electrochemistry.
main p.5-6 · 3.3 · Fig. 4; Fig. 5 · Linked to 4 structured results
N-PCD is the conductive MOF-derived component that assists electron transfer between the molecular probe and electrode surface.
Caveat: Conductivity is inferred from electrochemical current/Rct changes; no standalone bulk conductivity value is reported.
main p.1 · Abstract · Linked to 3 structured results
The synthesised ZIF-8 was high-purity ZIF-8 by XRD, with no other impurity peaks reported.
Caveat: Based on authors' XRD assignment; no CIF or raw diffraction file supplied.
main p.3 · 3.1 · Fig. 2A · Linked to 1 structured result
AuNPs/N-PCD/GCE provides more effective surface area and active sites than bare GCE, improving biomolecule loading and sensor sensitivity.
Caveat: Surface area is electrochemically effective area from chronocoulometry, not BET porosity.
main p.5 · 3.3 Electrochemical study · Fig. 4B · Linked to 2 structured results
The hollow, irregular-edged N-PCD morphology is favourable for AuNP deposition.
Caveat: This is an author interpretation from microscopy, not a quantified deposition metric.
main p.3 · 3.1 Characterization · Fig. 1C,D · Linked to 1 structured result
Calcination of ZIF-8 produces graphitic/disordered N-PCD carbon, evidenced by broad XRD carbon peaks and Raman D/G bands.
Caveat: Graphitic character is structural/spectroscopic, not direct conductivity measurement.
main p.3 · 3.1 Characterization · Fig. 2B,D · Linked to 5 structured results
Names and aliases are kept exactly within the paper’s own identity model.
| Material | Composition | Structure context | Source |
|---|---|---|---|
| AuNPs/N-PCD/glassy-carbon electrode interface | Au nanoparticles electrodeposited on N-PCD coated GCEAu nanoparticles plus MOF-derived N-PCD · Not applicable after carbonisation; N-doped carbon scaffold | unknown · CompositeComposite electrode interface formed by drop-coated N-PCD and electrodeposited AuNPs. | main p.2-6 · 2.3 Fabrication of the biosensor; 3.3 Electrochemical study · Fig. 4; Fig. 5 |
| N-doped porous carbon dodecahedron (N-PCD) | N-doped carbon derived from ZIF-8No retained framework metal node; derived by calcination of Zn-based ZIF-8 · Carbon/nitrogen framework derived from 2-methylimidazolate | 3D · DerivedMOF-derived hollow/irregular-edged porous carbon dodecahedron with graphitic carbon signatures by XRD and Raman. | main p.1-3 · Abstract; 2.1 Preparation of ZIF-8 and N-PCD · Scheme 2; Fig. 1B-D; Fig. 2B,D |
| Tetrahedral DNA nanostructure probe | Four-strand T4-DNA capture probe with thiol groupsDNA strands a, b, c and d | 3D · Model SystemThree-dimensional tetrahedral DNA nanostructure; AFM shows triangular particles after dehydration. | main p.3-5 · 2.2 Preparation of T4-DNA probe; 3.2 T4-DNA characterization · Fig. 3; Table S1 |
| ZIF-8 zeolitic imidazolate framework precursorBrowse family: ZIF-8 / Zn(mIm)₂ | Zn(2-methylimidazolate)2, prepared from Zn(CH3COO)2 and 2-methylimidazoleZn nodes from zinc acetate · 2-methylimidazole / 2-methylimidazolate | 3D · PristineZIF-8 phase assigned by XRD peaks indexed to reported ZIF-8 crystal planes; used as the MOF precursor for N-PCD. | main p.2-3 · 2.1 Preparation of ZIF-8 and N-PCD; 3.1 Characterization · Fig. 2A |
Sample form, processing state and composition status define the context for measurements.
| Sample | Form and role | Processing and geometry | Source |
|---|---|---|---|
| Au/GCE electrode without N-PCDresearch_0866__mat__au_npcd_interface | Electrode · Pristine Control · Composite | Au-modified GCE without N-PCD, used as no-N-PCD DPV comparator.glassy carbon electrode (GCE) | main p.5 · 3.3 Electrochemical study · Fig. 4C,D |
| AuNPs/N-PCD/GCE composite electroderesearch_0866__mat__au_npcd_interface | Electrode · Composite Sample · Composite | N-PCD/GCE placed in 0.1% HAuCl4 containing 0.1 M KCl and AuNPs electrodeposited by i-t at -0.2 V for 30 s.glassy carbon electrode (GCE) | SI p.4 · Electrochemical measurements · Fig. S1A |
| Dual miRNA-21/miRNA-141 biosensorresearch_0866__mat__au_npcd_interface | Electrode · Composite Sample · Composite | T4-DNA recognition sequence and signal probe changed to detect miRNA-21 by MB and miRNA-141 by Fc.glassy carbon electrode (GCE) | SI p.11 · Analytical performance of biosensors · Fig. S7; Fig. S8 |
| DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensorresearch_0866__mat__au_npcd_interface | Electrode · Composite Sample · Composite | T4-DNA immobilised by Au-S bonding, MCH blocked, miRNA hybridised, then DNA1-Fc/DNA2-MB HCR probes added.glassy carbon electrode (GCE) | main p.3-7 · 2.3 Fabrication; 3.4 Analytical performance · Scheme 1; Fig. 6 |
| Bare glassy carbon electroderesearch_0866__mat__au_npcd_interface | Electrode · Pristine Control · Unknown | Polished bare GCE used as electrochemical control.glassy carbon electrode (GCE) | main p.3-6 · 2.3; 3.3 · Fig. 4B; Fig. 5 |
| N-PCD modified glassy carbon electroderesearch_0866__mat__n_pcd | Electrode · Target Sample · Derived Carbon | 8 uL of 1 mg/mL N-PCD solution drop-coated onto polished GCE and dried.glassy carbon electrode (GCE) | main p.3-5 · 2.3; 3.3 · Fig. 4A; Fig. 5A,C |
| Calcined N-PCD powderresearch_0866__mat__n_pcd | Powder · Target Sample · Derived Carbon | ZIF-8 calcined under N2 to 800 deg C and annealed to room temperature. | main p.2-4 · 2.1; 3.1 · Fig. 1B-D; Fig. 2B,D |
| Single-signal Fc miRNA-21 biosensorresearch_0866__mat__au_npcd_interface | Electrode · Composite Sample · Composite | Single Fc-labelled signalling probe variant of the T4-DNA/AuNPs/N-PCD/GCE sensor.glassy carbon electrode (GCE) | SI p.9 · Analytical performance of biosensors · Fig. S3; Fig. S4 |
| Single-signal MB miRNA-21 biosensorresearch_0866__mat__au_npcd_interface | Electrode · Composite Sample · Composite | Single MB-labelled signalling probe variant of the T4-DNA/AuNPs/N-PCD/GCE sensor.glassy carbon electrode (GCE) | SI p.10 · Analytical performance of biosensors · Fig. S5; Fig. S6 |
| Assembled T4-DNA probe solutionresearch_0866__mat__t4_dna_probe | Unknown · Composite Component · Model | Four DNA precursor solutions mixed equally, heated to 95 deg C, annealed on ice, stored at -20 deg C. | main p.3 · 2.2 Preparation of T4-DNA probe · Fig. 3; Table S1 |
| Synthesised ZIF-8 powderresearch_0866__mat__zif8_precursor | Powder · Pristine Control · Pristine Framework | Methanol precipitation product collected by centrifugation, ethanol washed and vacuum dried. | main p.2-3 · 2.1; 3.1 · Fig. 1A; Fig. 2A,C |