Electrochemistry Application — Target-triggered hybridization chain reaction for ultrasensitive dual-signal miRNA detection

4 measurement groups · 19 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Chronocoulometry

AuNPs/N-PCD/GCE composite electrode · Electrode

Q-t and Q-t^1/2 curves used to calculate effective specific surface area; compared GCE and AuNPs/N-PCD/GCE. SI electrolyte: 0.1 M PBS pH 7.4 containing 0.1 mM [Fe(CN)6]3- and 1 M KCl.

Geometry: GCE and AuNPs/N-PCD/GCE
Context: composite electrode versus bare GCE control
Measurement source: SI p.4 · Electrochemical measurements · Fig. 4B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
AuNPs/N-PCD/GCE effective specific surface area	0.17 cm2	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4B
GCE effective specific surface area	0.059 cm2	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4B
Chronocoulometry diffusion coefficient used	7.6 x 10-6 cm2/s	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4B

Cyclic voltammetry (CV) at different scan rates

Voltammetry

N-PCD modified glassy carbon electrode · Electrode

CV of N-PCD/GCE; scan rate 20-200 mV/s at 20 mV/s intervals. SI CV electrolyte: 0.1 M PBS pH 7.4 with 5 mM [Fe(CN)6]3-/4- and 0.1 M KCl, -0.2 to 0.6 V at 100 mV/s for standard CV.

Geometry: N-PCD/GCE electrode
Context: MOF-derived carbon electrode
Measurement source: SI p.3-4 · Electrochemical measurements · Fig. 4A

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
N-PCD/GCE Epa at high scan rate	0.273 V	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A
Anodic peak potential shift	Epa shifts from 0.250 V to 0.273 V	—	—	Text Range	main p.4-5 · 3.3 · Fig. 4A
N-PCD/GCE Epa at low scan rate	0.250 V	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A
N-PCD/GCE Epc at high scan rate	0.153 V	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A
Cathodic peak potential shift	Epc shifts from 0.179 V to 0.153 V	—	—	Text Range	main p.4-5 · 3.3 · Fig. 4A
N-PCD/GCE Epc at low scan rate	0.179 V	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A
N-PCD/GCE Ipa/Ipc ratio	about 1	—	—	Text Approximate	main p.4 · 3.3 · Fig. 4A
N-PCD/GCE anodic peak current regression	Ipa (uA) = 2.43 + 15.44 v^1/2	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A inset
N-PCD/GCE cathodic peak current regression	Ipc (uA) = 0.68-16.15 v^1/2	—	—	Text Exact Reported	main p.4 · 3.3 · Fig. 4A inset
CV scan-rate regression correlation coefficient	0.999	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4A inset
CV scan-rate range	20 mV/s to 200 mV/s with 20 mV/s interval	—	—	Caption Range	main p.5 · Fig. 4 caption · Fig. 4A

Stepwise cyclic voltammetry (CV)

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

CV of electrodes modified with GCE, N-PCD/GCE, AuNPs/N-PCD/GCE, T4-DNA, MCH, miRNA and DNA probes.

Geometry: Sequentially modified GCE
Context: stepwise composite sensor construction
Measurement source: main p.5-6 · 3.3 Electrochemical study on signal amplification by N-PCD · Fig. 5A,B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
CV peak current increase after AuNP deposition	further increases by 10%	—	—	Text Exact Reported	main p.6 · 3.3 · Fig. 5A
CV peak current effect of N-PCD	peak current of GCE modified with N-PCD increases significantly	—	—	Text Qualitative	main p.6 · 3.3 · Fig. 5A

Electrochemical impedance spectroscopy (EIS)

Impedance spectroscopy

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Nyquist/EIS comparison of sequential electrode modification steps. SI EIS electrolyte: 0.1 M PBS pH 7.0 with 5 mM [Fe(CN)6]3-/4- and 0.1 M KCl, 0.1 to 10^4 Hz.

Geometry: Sequentially modified GCE
Context: stepwise composite sensor construction
Measurement source: SI p.4 · Electrochemical measurements · Fig. 5C,D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
AuNP deposition effect on Rct	further reduces the Rct value	—	—	Text Qualitative	main p.6 · 3.3 · Fig. 5C
GCE charge-transfer resistance Rct	250 ohm	—	—	Text Exact Reported	main p.6 · 3.3 · Fig. 5C
N-PCD/GCE charge-transfer resistance Rct	130 ohm	—	—	Text Exact Reported	main p.6 · 3.3 · Fig. 5C