Sensing Application — Target-triggered hybridization chain reaction for ultrasensitive dual-signal miRNA detection

11 measurement groups · 45 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

DPV detection in total RNA extracted from L02 and MCF-7 cells

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Constructed electrochemical sensor used to detect miRNA-21 in total RNA from cell samples.

Atmosphere: N2 for DPV
Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite in biological matrix
Measurement source: main p.7 · 3.4 Analytical performance · Fig. 6D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
L02 cell Fc DPV current	approximately 1.45 uA from Fig. 6D bar	—	visual estimate from bar height	Figure Axis Approximate	main p.7 · 3.4 · Fig. 6D
L02 cell MB DPV current	approximately 0.75 uA from Fig. 6D bar	—	visual estimate from bar height	Figure Axis Approximate	main p.7 · 3.4 · Fig. 6D
MCF-7 cell Fc DPV currentMarked as a best value within this paper	approximately 3.55 uA from Fig. 6D bar	—	visual estimate from bar height	Figure Axis Approximate	main p.7 · 3.4 · Fig. 6D
Cell-sample miRNA-21 response	MCF-7 dual-signal peak current significantly higher than L02	—	—	Text Qualitative	main p.7 · 3.4 · Fig. 6D
MCF-7 cell MB DPV current	approximately 3.00 uA from Fig. 6D bar	—	visual estimate from bar height	Figure Axis Approximate	main p.7 · 3.4 · Fig. 6D

Differential pulse voltammetry (DPV)

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

DPV compared final electrode with N-PCD against no-N-PCD Au/GCE control at 2000 fM miRNA-21. SI DPV: 10 mM PBS pH 7.0 under N2, -0.6 to 0.4 V.

Atmosphere: N2 for DPV
Geometry: DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE versus Au/GCE control
Context: application composite with no-N-PCD control
Measurement source: SI p.4 · Electrochemical measurements · Fig. 4D

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Fc DPV response increase from N-PCD	147.4%	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4D
MB DPV response increase from N-PCD	200.5%	—	—	Text Exact Reported	main p.5 · 3.3 · Fig. 4D

DPV calibration for simultaneous miRNA-21 and miRNA-141

Voltammetry

Dual miRNA-21/miRNA-141 biosensor · Electrode

MB signal detects miRNA-21 and Fc signal detects miRNA-141 for 0-10000 fM target concentrations.

Atmosphere: N2 for DPV
Geometry: Dual-target T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite multi-target variant
Measurement source: SI p.7-11 · Analytical performance of biosensors · Fig. S7; Fig. S8

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Multi-target Fc141 calibration equation	IFc141 (uA)=1.15 log(c)+17.19	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B
Multi-target Fc141 correlation coefficient	0.998	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B
Multi-target MB21 calibration equation	IMB21 (uA)=0.70 log(c)+10.25	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B
Multi-target MB21 correlation coefficient	0.997	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B
Multi-target miRNA-141 LOD	3.49 fM	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B
Multi-target miRNA-21 LOD	6.22 fM	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S8B

Performance comparison tables

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

SI Tables S2 and S3 compare this sensor with literature miRNA-21 sensors and material platforms.

Geometry: T4-DNA/Au/N-PCD+HCR DPV sensor
Context: application composite benchmark
Measurement source: SI p.14-15 · Tables S2 and S3 · Table S2; Table S3

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Table S2 this-work LOD	3.74 fM	—	—	SI Table Exact Reported	SI p.14 · Table S2 · Table S2
Table S2 this-work linear range	5 fM-10000 fM	—	—	SI Table Range	SI p.14 · Table S2 · Table S2
Table S3 N-PCD material LOD	3.74 fM	—	—	SI Table Exact Reported	SI p.15 · Table S3 · Table S3

DPV calibration for miRNA-21

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

DPV peak currents of Fc and MB probes recorded for 0, 5, 10, 50, 100, 500, 2000, 5000 and 10000 fM miRNA-21.

Atmosphere: N2 for DPV
Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite
Measurement source: main p.6-7 · 3.4 Analytical performance · Fig. 6A,B

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Dual-signal Fc calibration equation	IFc (uA) = 1.55 log(c)+23.57	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B inset
Dual-signal MB calibration equation	IMB (uA) = 1.44 log(c)+21.40	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B inset
Dual-signal Fc calibration correlation coefficient	0.998	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B inset
Dual-signal MB calibration correlation coefficient	0.996	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B inset
Dual-signal miRNA-21 linear rangeMarked as a best value within this paper	5-1.0 x 10^4 fM	—	—	Text Range	main p.1 · Abstract · Fig. 6B
Dual-signal Fc miRNA-21 LODMarked as a best value within this paper	1.92 fM	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B
Dual-signal MB miRNA-21 LODMarked as a best value within this paper	3.74 fM	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6B; Table S2; Table S3

DPV reproducibility and storage stability

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Six electrodes measured for reproducibility; five electrodes stored at 4 deg C for 10 days and tested every 2 days.

Temperature: 277
Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite
Measurement source: main p.7 · 3.4 Analytical performance · Fig. S2A referenced

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Electrode-to-electrode reproducibility RSD	4.9%	—	—	Text Exact Reported	main p.7 · 3.4
Fc signal retention after 10 days	98.7%	—	—	Text Exact Reported	main p.7 · 3.4 · Fig. S2A
MB signal retention after 10 days	97.4%	—	—	Text Exact Reported	main p.7 · 3.4 · Fig. S2A

DPV selectivity test

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

DPV responses to 2000 fM miRNA-21 and interfering RNA sequences.

Atmosphere: N2 for DPV
Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite
Measurement source: main p.6-7 · 3.4 Analytical performance · Fig. 6C; Table S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Selectivity interferent concentration	2000 fM	—	—	Text Exact Reported	main p.6 · 3.4 · Fig. 6C
Selectivity against interfering RNAs	no significant difference from absence of target	—	—	Text Qualitative	main p.6 · 3.4 · Fig. 6C

Serum recovery experiment

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Serum samples from healthy subjects and breast cancer patients centrifuged and diluted 10 times with 10 mM PBS pH 7.4; recoveries summarised in Table S4.

Atmosphere: N2 for DPV
Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite in serum matrix
Measurement source: main p.7 · 3.4 Analytical performance · Table S4 referenced

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Maximum serum recovery	106.57%	—	—	SI Table Exact Reported	SI p.16 · Table S4 · Table S4
Minimum serum recovery	92.16%	—	—	SI Table Exact Reported	SI p.16 · Table S4 · Table S4
Serum recovery range	92.16% to 106.57%	—	—	Text Range	main p.7 · 3.4 · Table S4 referenced
Serum sample 3 endogenous miRNA-21 found	33.5 +/- 1.7 fM	—	+/- 1.7 fM	SI Table Exact Reported	SI p.16 · Table S4 · Table S4
Serum sample 4 endogenous miRNA-21 found	36.9 +/- 2.4 fM	—	+/- 2.4 fM	SI Table Exact Reported	SI p.16 · Table S4 · Table S4

DPV optimisation of sensor construction variables

Voltammetry

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Optimised Au deposition time, miRNA incubation time, DNA1-Fc/DNA2-MB concentration and co-incubation time using 2000 fM miRNA.

Geometry: Dual-signal T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite
Measurement source: SI p.5-8 · Optimization of sensor · Fig. S1

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Selected Au deposition time	30 s	—	—	Text Exact Reported	SI p.5 · Optimization of sensor · Fig. S1A
Optimal Au deposition time by Fc signal	25 s	—	—	Text Exact Reported	SI p.5 · Optimization of sensor · Fig. S1A
Optimal/selected Au deposition time by MB signal	30 s	—	—	Text Exact Reported	SI p.5 · Optimization of sensor · Fig. S1A
Selected miRNA incubation time	70 min	—	—	Text Exact Reported	SI p.5 · Optimization of sensor · Fig. S1B
Selected DNA1-Fc/DNA2-MB co-incubation time	70 min	—	—	Text Exact Reported	SI p.6 · Optimization of sensor · Fig. S1D
Selected DNA1-Fc/DNA2-MB concentration	1.0 mM	—	—	Text Exact Reported	SI p.5-6 · Optimization of sensor · Fig. S1C

DPV calibration for miRNA-21 using Fc single signal

Voltammetry

Single-signal Fc miRNA-21 biosensor · Electrode

Fc DPV signal for 0, 5, 10, 50, 100, 500, 2000, 5000 and 10000 fM miRNA-21.

Atmosphere: N2 for DPV
Geometry: Single-signal Fc T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite single-signal comparator
Measurement source: SI p.6-9 · Analytical performance of biosensors · Fig. S3; Fig. S4

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Single-signal Fc calibration equation	IFc (uA)=1.58 log(c)+23.94	—	—	Text Exact Reported	SI p.6-7 · Analytical performance of biosensors · Fig. S4B
Single-signal Fc miRNA-21 LOD	2.08 fM	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S4B
Single-signal Fc calibration correlation coefficient	0.995	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S4B

DPV calibration for miRNA-21 using MB single signal

Voltammetry

Single-signal MB miRNA-21 biosensor · Electrode

MB DPV signal for 0, 5, 10, 50, 100, 500, 2000, 5000 and 10000 fM miRNA-21.

Atmosphere: N2 for DPV
Geometry: Single-signal MB T4-DNA/AuNPs/N-PCD/GCE biosensor
Context: application composite single-signal comparator
Measurement source: SI p.7-10 · Analytical performance of biosensors · Fig. S5; Fig. S6

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Single-signal MB calibration equation	IMB (uA)=1.63 log(c)+24.20	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S6B
Single-signal MB miRNA-21 LOD	3.89 fM	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S6B
Single-signal MB calibration correlation coefficient	0.995	—	—	Text Exact Reported	SI p.7 · Analytical performance of biosensors · Fig. S6B