cMOF Evidence Base
Other — Target-triggered hybridization chain reaction for ultrasensitive dual-signal miRNA detection

Measurement evidence

Other

Target-triggered hybridization chain reaction for ultrasensitive dual-signal miRNA detection · Wang Y., Feng H., Huang K. et al. · Biosensors and Bioelectronics · 2022 · 114572

2 measurement groups · 5 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

Agarose gel electrophoresis and OD260/280 RNA purity check

DNA1-Fc + DNA2-MB/miRNA/MCH/T4-DNA/AuNPs/N-PCD/GCE biosensor · Electrode

Total RNA extracted from L02 cells (channels 1-4) and MCF-7 cells (channels 5-8), assessed by electropherogram and OD260/280.

Geometry
Cell RNA sample preparation for biosensor testing
Context
biological matrix quality control
Measurement source
SI p.6-8 · Characterization of RNA purity · Fig. S2B
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
RNA electrophoresis bands5 S, 18 S and 28 S bands observed——Text
Qualitative		SI p.6 · Characterization of RNA purity · Fig. S2B
RNA OD260/280 range2.02-2.18——Text
Range		SI p.6 · Characterization of RNA purity · Fig. S2B

3.5% agarose gel electrophoresis and atomic force microscopy (AFM)

Atomic force microscopy

Assembled T4-DNA probe solution · Unknown

Characterisation of T4-DNA assembly; AFM instrument Dimension Icon per SI.

Context
non-MOF biomolecular scaffold component
Measurement source
main p.3-5 · 3.2 T4-DNA characterization · Fig. 3
PropertyReported valueNormalised valueUncertaintyOrigin and qualitySource
T4-DNA AFM apparent morphologytriangular particles of uniform size——Text
Qualitative		main p.3 · 3.2 · Fig. 3B
T4-DNA gel migrationT4-DNA in lane 6 has a three-dimensional structure and slower migration——Text
Qualitative		main p.3 · 3.2 · Fig. 3A
T4-DNA assemblyT4-DNA lane 6 has slower migration; AFM shows triangular particles of uniform size——Text
Qualitative		main p.3 · 3.2 · Fig. 3