Electrochemistry Application — Novel 2D CuFe-MOF-based immunoprobe: Addressing antifouling electrochemical immunosensing inadequate sensitivity challenge

4 measurement groups · 12 results

Reported values remain attached to the sample, method, conditions, extraction quality and source location that produced them.

GCE-Gel-Ab1-Antigen-Probe immunosensor · Electrode

10 mM PBS including 0.1 M KCl, pH 7.4; sequential GCE, GCE-Gel, GCE-Gel-Ab1, GCE-Gel-Ab1-Antigen, GCE-Gel-Ab1-Antigen-Probe.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Current response after CuFe-MOF immunoprobe binding	dramatic growth in current response observed after 2D immunoprobes were bonded	—	—	Text Qualitative	6 · 3.4 Electrochemical characterization · Fig. 3A

2D CuFe-MOF · Nanosheet

Scan rates 10-500 mV s-1 in 1 mM K3[Fe(CN)6] containing 0.1 M KCl; Randles-Sevcik equation.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Bare GCE effective electrode area, conflicting assignment	0.07 cm2 (main-text inferred; SI caption order conflicts)	—	—	Text Uncertain	1.4 Supplementary Figures · Fig. S3
Effective electrode area modified with 2D CuFe-MOFMarked as a best value within this paper	0.077 cm2	—	—	Text Exact Reported	5 · 3.3 Characterization of immunoprobe · Fig. S3

GCE-Gel · Electrode

Bare, BSA-modified and gel-modified GCE exposed to real human serum dilutions.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
Bare GCE degree of contamination at 100% serum	1962.4%	—	—	Text Exact Reported	7 · 3.5 Antifouling performance · Fig. 4
Bare GCE degree of contamination at 20% serum	377.7%	—	—	Text Exact Reported	6 · 3.5 Antifouling performance · Fig. 4
Bare GCE degree of contamination at 40% serum	646.5%	—	—	Text Exact Reported	6 · 3.5 Antifouling performance · Fig. 4
Bare GCE degree of contamination at 60% serum	813.9%	—	—	Text Exact Reported	6 · 3.5 Antifouling performance · Fig. 4
Bare GCE degree of contamination at 80% serum	1061.6%	—	—	Text Exact Reported	6 · 3.5 Antifouling performance · Fig. 4
BSA-modified GCE degree of contamination at 100% serum	approximately 780%	—	visual estimate from bar height	Visual Estimate Approximate	6 · 3.5 Antifouling performance · Fig. 4
Gel-modified GCE degree of contamination at 100% serumMarked as a best value within this paper	39.3%	—	—	Text Exact Reported	7 · 3.5 Antifouling performance · Fig. 4
Gel-modified GCE contamination range across serum dilutionsMarked as a best value within this paper	approximately 20-39.3% from 20-100% serum	—	visual estimate for 20-80%; 39.3% text-reported at 100%	Visual Estimate Range	6 · 3.5 Antifouling performance · Fig. 4

GCE-Gel-Ab1-Antigen-Probe immunosensor · Electrode

5 mM [Fe(CN)6]4-/3- including 0.1 M KCl; sequential interface modification.

Property	Reported value	Normalised value	Uncertainty	Origin and quality	Source
EIS construction verification	EIS detections consistent with CV, indicating successful construction	—	—	Text Qualitative	6 · 3.4 Electrochemical characterization · Fig. 3B